main olfactory system; mice; sexual behavior; sex recognition; zinc sulfate
Abstract :
[en] We studied the contribution of the main olfactory system to mate recognition and sexual behavior in female mice. Female mice received an intranasal irrigation of either a zinc sulfate (ZnSO4) solution to destroy the main olfactory epithelium (MOE) or saline (SAL) to serve as control. ZnSO4-treated female mice were no longer able to reliably distinguish between volatile as well as nonvolatile odors from an intact versus a castrated male. Furthermore, sexual behavior in mating tests with a sexually experienced male was significantly reduced in ZnSO4-treated female mice. Vomeronasal function did not seem to be affected by ZnSO4 treatment: nasal application of male urine induced similar levels of Fos protein in the mitral and granule cells of the accessory olfactory bulb (AOB) of ZnSO4 as well as SAL-treated female mice. Likewise, soybean agglutinin staining, which stains the axons of vomeronasal neurons projecting to the glomerular layer of the AOB was similar in ZnSO4-treated female mice compared to SAL-treated female mice. By contrast, a significant reduction of Fos in the main olfactory bulb was observed in ZnSO4-treated females in comparison to SAL-treated animals, confirming a substantial destruction of the MOE. These results show that the MOE is primarily involved in the detection and processing of odors that are used to localize and identify the sex and endocrine status of conspecifics. By contrast, both the main and accessory olfactory systems contribute to female sexual receptivity in female mice.
Research center :
Giga-Neurosciences - ULiège
Disciplines :
Neurosciences & behavior Neurology
Author, co-author :
Keller, Matthieu ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Douhard, Quentin ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Baum, M. J.
Bakker, Julie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Language :
English
Title :
Destruction of the main olfactory epithelium reduces female sexual behavior and olfactory investigation in female mice
Publication date :
May 2006
Journal title :
Chemical Senses
ISSN :
0379-864X
eISSN :
1464-3553
Publisher :
Oxford Univ Press, Oxford, United Kingdom
Volume :
31
Issue :
4
Pages :
315-323
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NICHD - National Institutes of Health. Eunice Kennedy Shriver National Institute of Child Health and Human Development [US-MD] [US-MD]
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