Modulatory Effect of Neuropeptide Y on Acetylcholine-Induced Oedema and Vasoconstriction in Isolated Perfused Lungs of Rabbit

[en] 1. The modulatory role of neuropeptide Y (NPY) on pulmonary oedema induced by acetylcholine and capsaicin was investigated. The effects of NPY on the haemodynamic response to acetylcholine, phenylephrine and substance P were also investigated. 2. Isolated, ventilated, exsanguinated lungs of the rabbit were perfused with a constant flow of recirculating blood-free perfusate. The double/arterial/venous occlusion method was used to partition the total pressure gradient (delta Pt) into four components: the arterial gradient (delta Pa), the pre- and post-capillary gradients (respectively delta Pa' and delta Pv') and the venous pressure gradient (delta Pv). Endothelial permeability was evaluated by measuring the capillary filtration coefficient (Kf,c). 3. Acetylcholine (10(-8) M to 10(-4) M) and substance P (SP, 10(-10) M to 10(-6) M) induced a concentration-dependent increase in the Kf,c. Capsaicin (10(-4) M) and 5-hydroxytryptamine (5-HT) (10(-4) M) also increased this parameter. NPY (10(-8) M) completely inhibited the effects of acetylcholine and capsaicin on the Kf,c, without preventing the effects of substance P and 5-HT. 4. Acetylcholine induced concentration-dependent vasoconstriction in the precapillary segment. The effect was inhibited by NPY and aspirin, an inhibitor of cyclo-oxygenase, while ketanserin, a 5-HT2 receptor antagonist, and SR140333, a new NK1 antagonist, had no protective effect. Phenylephrine increased delta Pa at high concentration, an effect also inhibited by NPY and aspirin. Substance P had no significant haemodynamic effect. When injected together with NPY, substance P (10(-6) M) induced a significant increase in the total pressure gradient.(ABSTRACT TRUNCATED AT 250 WORDS)

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Delaunois, Annie ; Université de Liège - ULiège > Relations académiques et scientifiques (Méd. vétérinaire)

Gustin, Pascal ; Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie

Dessy-Doize, C.

Ansay, Michel ; Université de Liège - ULiège > Services généraux (Faculté de médecine vétérinaire) > Relations académiques et scientifiques (Méd. vétérinaire)

Language :

English

Title :

Modulatory Effect of Neuropeptide Y on Acetylcholine-Induced Oedema and Vasoconstriction in Isolated Perfused Lungs of Rabbit

Publication date :

1994

Journal title :

British Journal of Pharmacology

ISSN :

0007-1188

Publisher :

Wiley-Blackwell, United States

Volume :

113

Issue :

Pages :

973-81

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 16 March 2009

Statistics

Number of views

75 (7 by ULiège)

Number of downloads

2 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

publications

supporting

mentioning

contrasting

Smart Citations

Citing PublicationsSupportingMentioningContrasting

View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Bibliography

ALTIERE R.J., KIRITSY‐ROY J.A., CATRAVAS J.D. (1986) Acetylcholine‐induced contractions in isolated rabbit pulmonary arteries: role of thromboxane A2. J. Pharmacol. Exp. Ther. 236:535-541.
CATRAVAS J.D., BUCCAFUSCO J.J., EL‐KASHEF H. (1984) Effects of acetylcholine in the pulmonary circulation of rabbits. J. Pharmacol. Exp. Ther. 231:236-241.
CHAND N., ALTURA B.M. (1981) Acetylcholine and bradykinin relax intrapulmonary arteries by acting on endothelial cells: role in lung vascular diseases. Science 213:1376-1379.
DAWSON C.A., LINEHAN J.H., RICKABY D.A. (1982) Pulmonary microcirculatory hemodynamics. Ann. N.Y. Acad. Sci. 384:90-106.
DELAUNOIS A., GUSTIN P., ANSAY M. (1992) Altered capillary filtration coefficient in parathion‐ and paraoxon‐induced pulmonary edema in isolated and perfused rabbit lungs. Toxicol. Appl. Pharmacol. 116:161-169.
DELAUNOIS A., GUSTIN P., ANSAY M. (1993) Role of neuropeptides in acetylcholine‐induced oedema in isolated and perfused rabbit lungs. J. Pharmacol. Exp. Ther. 266:483-491.
DELAUNOIS A., GUSTIN P., ANSAY M. (1993) Effects of capsaicin on the endothelial permeability in isolated and perfused rabbit lungs. Fundam. Clin. Pharmacol. 7:81-91.
DELAUNOIS A., GUSTIN P., ANSAY M. (1994) Modulation of the acetylcholine‐ and substance P‐induced pulmonary oedema by calcitonin gene‐related peptide in the rabbit. J. Pharmacol. Exp. Ther. 270:30-36.
DRAKE R.E., GABEL J.C. (1981) Comparison of technic to measure pulmonary capillary filtration coefficient in dogs. Micro-vase. Res. 21:133-141.
EDVINSSON L., EKBLAD E., HÅKANSON R., WAHLESTEDT C. (1984) Neuropeptide Y potentiates the effect of various vasoconstrictor agents in rabbit blood vessels. Br. J. Pharmacol. 83:519-525.
EL‐KASHEF H., CATRAVAS J.D. (1991) The nature of muscarinic receptor subtypes mediating pulmonary vasoconstriction in the rabbit. Pulm. Pharmacol. 4:18-19.
EMONDS‐ALT X., DOUTREMEPUICH, HEAULME M., NELIAT G., SANTUCCI V., STEINBERG R., VILAIN P., BIHON D., DUCOUX J.P., PROIETTO V., Van BROECK D., SOUBRIE P., Le FUR G., BRELIERE (1993) In vitro and in vivo biological activities of SR140333, a novel potent non‐peptide tachykinin NK1 receptor antagonist. Eur. J. Pharmacol. 250:403-413.
FALLGREN B., ARLOCK P., EDVINSSON L. (1993) Neuropeptide Y potentiates noradrenaline‐evoked vasoconstriction by an intracellular calcium‐dependent mechanism. J. Autonom. Nerv. System 44:151-159.
FROSSARD N., BARNES P.J. (1987) m‐opioid receptors modulate noncholinergic constrictor nerves in guinea‐pig airways. Eur. J. Pharmacol. 141:519-522.
GRUNDEMAR L., GRUNDSTRSM N., JOHANSSON I.G.M., AN‐DERSSON R.G.G., HÅKANSON R. (1990) Suppression by neuropeptide Y of capsaicin‐sensitive sensory nerve‐mediated contraction in guinea pig airways. Br. J. Pharmacol. 99:473-476.
GRUNDEMAR L., HOGESTATT E.D. (1992) Unmasking the vasoconstrictor response to neuropeptide Y and its interaction with vasodilating agents in vitro. Eur. J. Pharmacol. 221:71-76.
GRUNDSTROM N., ANDERSSON R.G.G. (1985) In vivo demonstration of alpha‐2 adrenoceptor‐mediated inhibition of the excitatory noncholinergic neurotransmission in guinea pig airways. Naunyn-Schmied. Arch. Pharmacol. 328:236-240.
GUSTIN P., URBAIN B., PROUVOST, ANSAY M. (1994) Effects of ammonia on pulmonary hemodynamics and vascular permeability in pigs: interaction with endotoxins. Toxicol. Appl. Pharmacol. 125:17-26.
HAKIM T.S. (1988) Identification of constriction in large versus small vessels using the arterial‐venous and double‐occlusion techniques in isolated canine lungs. Respiration 54:61-69.
HAKIM T.S., MICHEL R.P., CHANG H.K. (1982) Partitioning of pulmonary vascular resistance in dogs by arterial and venous occlusion. J. Appl. Physiol. 52:710-715.
HAN C., ABEL P.W. (1987) Neuropeptide Y potentiates contraction and inhibits relaxation of rabbit coronary arteries. J. Cardiovasc. Pharmacol. 9:675-681.
HOFMAN W.F., EHRHART I.C., CATRAVAS J.D. (1985) Prostanoid inhibition potentiates the vasoconstrictive response to acetylcholine in dog lung. Fed. Proc 44:1755.
ICHINOSE M., BARNES P.J. (1989) Histamine H3‐receptors modulate nonadrenergic noncholinergic bronchoconstriction in guinea‐pig in vivo. Eur. J. Pharmacol. 174:49-55.
JOOS G.F.P., PAUWELS R.A.R., VAN DER STRAETEN M.E.R.P. (1988) The role of neuropeptides as neurotransmitters of nonadrenergic, noncholinergic nerves in bronchial asthma. Bull. Eur. Physiopathol. Respir. 23:619-637.
KERN D.F., KIVLEN C., MALIK A.G. (1984) Pulmonary capillary filtration coefficient (Kf, c): blood vs Albumin‐Ringer's. Mic-rovasc. Res. 27:248.
LACROIX J.S., ANGGAARD A., HOKFELT T., OHARE M.M., FAHRENKRUG J., LUNDBERG J.M. (1990) Neuropeptide Y: presence in sympathetic and parasympathetic innervation of the nasal mucosa. Cell. Tissue Res. 259:119-128.
LAZARIS Y.A., SEREBROVSKAYA L.A., CHEREPANOVA A.G. (1969) Study of reaction of pulmonary vascular bed to some vasoactive agents using serial contrast roetgenovasography. Cor Vasa 11:21-25.
LEBLANC G.G., TRIMMER B.A., LANDIS S.C. (1987) Neuropeptide Y‐like immunoreactivity in rat cranial parasympathetic neurons: coexistence with vasoactive intestinal peptide and choline acetyltransferase. Proc. Natl. Acad. Sci. U.S.A. 84:3511-3515.
LUNDBERG J.M., FAHRENKRUG J., HBKFELT T., MARTLING C.R., LARSSON O., TATEMOTO K., ANGGARD A. (1984) Coexistence of peptide HI and VIP in nerves regulating blood flow and bronchial smooth muscle tone in various mammals including man. Peptides 235:593-606.
LUNDBERG J.M., MARTLING C.R., SARIA A., FOLKERS K., ROSELL S. (1983) Cigarette smoke induced oedema due to activation of capsaicin‐sensitive vagal afferents and substance P release. Neurosci. 10:1361-1368.
LUNDBERG J.M., PERNOW J., TATEMOTO K., DAHLOF C. (1985) Pre‐ and postjunctional effects of NPY on sympathetic control of rat femoral artery. Acta Physiol. Scand. 123:511-517.
LUNDBERG J.M., TERENIUS L., HBKFELT T., MARTLING C.R., TATEMOTO K., MUTT V., POLAK J., BLOOM S.R., GOLDSTEIN M. (1982) Neuropeptide Y (NPY)‐like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol. Scand. 116:477-480.
MACMURTRY I.F.E., WEIR E.K., REEVES J.T., WILL D.H., GROVER R.F. (1976) Prostaglandin synthesis does not mediate the pulmonary vasodilatory effect of acetylcholine. Prostaglandins 11:63-69.
MATRAN R., MARTLING, LUNDBERG J.M. (1989) Inhibition of cholinergic and non‐adrenergic, noncholinergic bronchoconstriction in the guinea pig mediated by neuropeptide Y and alpha 2‐adrenoceptors and opiate receptors. Eur. J. Pharmacol. 163:15-23.
NANDIWADA P.A., HYMAN A.L., KADOWITZ P.J. (1983) Pulmonary vasodilator responses to vagal stimulation and acetylcholine in the cat. Circ. Res. 53:86-95.
NI H. (1990) The pulmonary vascular responses to peripheral and central applications of acetylcholine. Eur. J. Pharmacol. 181:167-174.
PALMER R.J.M., FERRIGE A.G., MONCADA S. (1987) Nitric oxide release accounts for the biological activity of endothelial derived relaxing factor. Nature 327:524-526.
STRETTON C.D., BARNES P.J. (1988) Modulation of cholinergic neurotransmission in guinea pig trachea by neuropeptide Y. Br. J. Pharmacol. 93:672-678.
SUMITA T., ISHIKAWA N., HASHIBA Y., TAKAGI K., SATAKE T. (1989) 5‐Hydroxytryptamine receptor subtypes participating in pulmonary edema in dogs. Eur. J. Pharmacol. 164:69-75.
TATEMOTO K. (1982) Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc. Natl. Acad. Sci. U.S.A. 79:5485-5489.
T‐BENCHEKROUN M., FOURNIER A., ST‐PIERRE S., CADIEUX A. (1992) Inhibitory action of neuropeptide Y on agonist‐induced responses in isolated guinea pig trachea. Eur. J. Pharmacol. 216:421-428.
TOTH P.T., BINDOKAS V.P., BLEAKMAN D., COLMERS W.F., MILLER R.J. (1993) Mechanism of presynaptic inhibition by neuropeptide Y at sympathetic nerve terminals. Nature 364:635-638.
UDDMAN R., SUNDLER F., EMSON P. (1984) Occurrence and distribution of neuropeptide Y‐immunoreactive nerves in the respiratory tract and middle ear. Cell. Tiss. Res. 237:321-327.
WAHLESTEDT C., EDVINSSON L., EKBLAD E., HÅKANSON R. (1985) Neuropeptide Y potentiates noradrenaline‐evoked vasoconstriction: mode of action. J. Pharmacol. Exp. Ther. 234:735-741.
WALLENSTEIN S., ZUCKER C.L., FLEISS J.L. (1980) Some statistical methods useful in circulation research. Circ. Res. 47:1-9.
WANGENSTEEN O.D., LYSAKER E., DAVARYN P. (1977) Pulmonary capillary filtration and flexion coefficients in the adult rabbit. Microvasc. Res. 14:81-87.
WHORTEN G.S., GUMBAY R.S., TANAKA D.T., GRUNSTEIN M.M. (1985) Opposing hemodynamic effects of substance P on pulmonary vasculature in rabbits. J. Appl. Physiol. 59:1098-1103.