Animals; Blastocyst/cytology/physiology; Cattle; Female; Motion Pictures as Topic
Abstract :
[en] The behaviour of the cow blastocyst in vitro was studied by time-lapse cinematography and analysed by morphometry. Three types of behaviour were observed: continuous expansion followed by hatching; discontinuous expansion interrupted by few contractions and followed by hatching; discontinuous expansion interrupted by several rapid contractions without hatching. This demonstrated that the pulsatile activity of the blastocyst is not a necessary condition of hatching but also that only a moderate pulsatile activity is compatible with normal hatching. The time of hatching in vitro corresponded approximately with the time of zona loss in vivo (9-10 days). Rupture of the zona occurred at any point of the trophoblast layer. Hatching by herniation through a reduced opening of the zona was occasionally observed. The behavior of the embryos from a particular animal was very similar but differences were noted between embryos from different animals.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Massip, A.
Mulnard, J.
Vanderzwalmen, P.
Hanzen, Christian ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Obstétr. et path. de la reprod. des équidés, rumin. et porcs
Ectors, Francis; Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Language :
English
Title :
The behaviour of cow blastocyst in vitro: cinematographic and morphometric analysis.