Ovum pick-up; Assisted reproduction; Medium responders; High responders; FSH; Cow
Abstract :
[en] In human IVF, the concept of 'low responders' is well known and generally defined as women with poor-response to gonadotrophin stimulation in a previous induction cycle. The objective of this retrospective study is to describe and characterise the concepts of 'low-, medium-, and high-response' and 'low, medium, and high responders' in bovine-assisted reproduction by analysing the OPU-IVF results obtained following 665 gonadotrophin-stimulated sessions conducted in 112 animals, nearly all of which were previously unsuccessful in traditional multiple ovulation and embryo transfer (MOET) programs. They were submitted to OPU and IVP between 1999 and 2003. In reference to these 665 OPU sessions, categories of response were defined based on the overall mean+/-S.D. follicles aspirated and COC obtained i.e., for follicles 14.7+/-9.8 and for COCs 11.7+/-8.1. So arbitrary cut-off values to define the categories of sessions were for follicles 5 and 25, and for COC 4 and 20. The three categories for follicles punctured in one session were therefore follicle low-response (FLR)<or=5 follicles, follicle medium-response (FMR) 6-24 follicles or follicle high-response (FHR)>or=25 follicles and for COCs recovered in one session COC low-response (CLR)<or=4 COC, COC medium-response (CMR) 5-19 COC or COC high-response (CHR)>or=20 COC. In addition, four categories of animals were also defined: (1) a low responder animal (LRA) had at least one OPU session in which FLR and CLR were observed (genuine low-response, see Section ); these animals did not have any high-response sessions, (2) a medium responder animal (MeRA) had only medium-responses, (3) a high responder animal (HRA) had at least one OPU session in which FHR and CHR were observed; these animals did not have any low-response sessions, and (4) mixed responder animals (MiRA) had both low and high-responses. Finally, we distinguished biological (animals) and technical (recovery rate and ultrasound resolution) causes of response differences. In 'low, high, medium and mixed reponders,' different results were obtained (p<0.05): mean follicle numbers (8.8+/-4.8a, 22.4+/-10.5c, 13.2+/-5.2b,15.1+/-10.2d), COC numbers (6.3+/-3.9a, 18.5+/-8.2c, 10.4+/-4b, 12.0+/-8.3d), embryo numbers (1.8+/-2.1a, 5.6+/-4.9c, 2.5+/-2.7b, 3.5+/-3.8d) and also for recovery rate (72%a, 83%b,79%, 79%) and percentage embryo development (29%, 30%a, 24%b, 29%). In conclusion, the results of this study demonstrate that variability in OPU results has technical (ultrasound resolution and recovery rate) as well as biological (animal) aspects. Selection of animals with extreme (high or low) follicle and COC production results allows us to distinguish three populations: 'low, medium, and high responders' to FSH stimulation.
Disciplines :
Veterinary medicine & animal health Biotechnology
Author, co-author :
De Roover, Rudolf; Universite de Liège, Faculté de Médecine Vétérinaire > Thériogenologie des animaux de production
Bols, P. E. J.
Genicot, Gaëtan; Université de Liège - ULiège
Hanzen, Christian ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Obstétr. et path. de la reprod. des équidés, rumin. et porcs
Language :
English
Title :
Characterisation of low, medium and high responders following FSH stimulation prior to ultrasound-guided transvaginal oocyte retrieval in cows
S.P. Lerner, W.V. Thayne, R.D. Baker, T. Hensche, S. Merdith, and S.K. Inskeep Age, dose of FSH and other factors affecting superovulation in Holstein cows J Anim Sci 1986 176 183
M. Kafi, and M.R. McGowan Factors associated with variation in the superovulatory response of cattle Anim Reprod Sci 48 1997 137 157
J.F. Hasler The current status of oocyte recovery, in vitro embryo production and embryo transfer in domestic animals with emphasis on the bovine J Anim Sci 76 1998 52 74
W.W. Thatcher, F. Moreira, J.E. Santos, R.C. Mattos, F.L. Lopes, and S.M. Pancari Effects of hormonal treatments on reproductive performance and embryo production Theriogenology 55 2001 75 89
D. Monniaux, D. Chupin, and J. Saumande Superovulatory responses of cattle Theriogenology 19 1983 55 82
J. Singh, M. Dominguez, R. Jaiswal, and G. Adams A simple ultrasound test to predict the superstimulatory response in cattle Theriogenology 62 2004 227 243
R.A. Cushman, J.C. DeSouza, V.S. Hedgpeth, and J.H. Britt Superovulatory response of one ovary is related to the micro- and macro-scopic population of follicles in the contralateral ovary of the cow Biol Reprod 60 1999 349 354
R.J. Mapletoft, K. Bennett Steward, and G.P. Adams Recent advances in the superovulation of cattle Reprod Nutr Dev 42 2002 601 611
M.C. Pieterse, K.A. Kappen, Th.A.M. Kruip, and T.A.M. Taverne Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries Theriogenology 30 1988 751 762
D. Bousquet, H. Twagiramungu, N. Morin, C. Brisson, C. Carboneau, and J. Durocher In vitro embryo production in the cow: an effective alternative to the conventional embryo production approach Theriogenology 51 1999 59 70
J.F. Hasler, W.B. Henderson, P.J. Hurtgen, Z.O. Jin, A.D. McCauly, and S.A. Mower Production, freezing and transfer of bovine IVF embryos and subsequent calving results Theriogenology 43 1995 141 152
C.R. Looney, B.R. Lindsey, C.L. Gonseth, and D.L. Johnson Commercial aspects of oocyte retrieval and in vitro fertilisation (IVF) for embryo production in problem cows Theriogenology 41 1994 67 72
J.E. Garcia, G.S. Jones, A.A. Acosta, and G. Wright Jr. Human menopausal gonadotriphin/human chorionic gonadotrophin follicular maturation for oocyte aspiration: phase II 1981 Fertil Steril 39 1983 174 179
S.D. Keay, N.H. Liversedge, R.S. Mathur, and J.M. Jenkins Assisted conception following poor ovarian response to gonadotrophin stimulation BJOG 100 1997 521 527
B.C. Tarlatzis, L. Zepiridis, and G. Grimbizis Bontis clinical management of low ovarian response to stimulation for IVF: a systematic review J Hum Reprod Updat 9 1 2003 61 76
R. Lawson, T. El-Toukhy, A. Kassab, A. Taylor, P. Braude, and J. Parsons Poor response to ovulation induction is a stronger predictor of early menopause than elevated basal FSH: a life table analysis Hum Reprod 18 2003 527 533
J.M. Jenkins, D.W. Davies, H. Devonport, F.W. Anthony, S.C. Gadd, and R.H. Watson Comparison of poor responders with good responders using a standard buserelin/human menopausal gonadotrophin regime for in-vitro fertilisation Hum Reprod 6 1991 918 921
P. Thiruppathi, S. Shatavi, J.A. Dias, E. Radwanska, and J.L. Luborsky Gonadotrophin receptor expression on human granulosa cells of low and normal responders to FSH Mol Hum Reprod 7 2001 697 704
J.L. Luborsky, P. Thiruppathi, B. Rivnay, R. Roussev, C. Coulam, and E. Radwanska Evidence for different aetiologies of low oestradiol response to FSH: age-related accelerated luteinisation of follicles or presence of ovarian auto-antibodies Hum Reprod 17 2002 2641 2649
R. De Roover, F. Derkenne, and C. Hanzen Effet d'un protocole de stimulation de la croissance folliculaire sur la production in vitro d'embryons chez la vache Ann Med Vet 2004 (in press)
P.E.J. Bols, M.T. Ysebaert, A. Lein, M. Coryn, A. Van Soom, and A. de Kruif Effects of long-term treatment with bovine somatotropin on follicular dynamics and subsequent oocyte and blastocyst yield in an OPU-IVF program Theriogenology 49 1998 983 995
A.S. Lopes, P. Lovendahl, and H. Allesen Assessment of number of oocytes retrieved from individual cows can be based on few collections Proceedings of the 19th scientific meeting of the European Embryo Transfer Association 2003 p. 182
Bols PEJ. Transvaginal ovum pick-up in the cow: technical and biological modifications. Ph.D. thesis. University of Ghent, Ghent, Belgium, 1997.
C. Galli, G. Crotti, C. Notari, P. Turini, R. Duchi, and G. Lazzari Embryo production by ovum pick up from live donors Theriogenology 55 2001 1341 1357
C.H. Hanzen, M. Pieterse, O. Scenczi, and M. Drost Relative accuracy of the identification of ovarian structures in the cow by ultrasonography and palpation per rectum Vet J 159 2000 161 170
J.G. Gong, T.A. Bramley, and R. Webb The effect of recombinant bovine somatotrophin on ovarian follicular growth and development in heifers J Reprod Fertil 97 2003 247 254
M.C. Pieterse, M.A.M. Taverne, Th.A.M. Kruip, and A.H. Willemse Detection of corpora lutea and follicles in cows: a comparison of transvaginal ultrasonography and rectal palpation Vet Rec 126 1990 552 554
E.R. Klinkert, F.J.M. Broekmans, C.W.N. Looman, and E.R. te Velde A poor response in the first in vitro fertilisation cycle is not necessarily related to a poor prognosis in subsequent cycles Fertil Steril 81 5 2004 1247 1253
R.J. Scaramuzzi, and J.F. Murray The nutrient requirements for the optimum production of gametes in assisted reproduction in ruminant animals Proc 10th Scientific Meeting AETE 1994 p. 85-103
S.G. Awadalla, C.L. Friedman, N.W. Chin, W. Dodds, J.M. Park, and M.H. Kim Follicular stimulation for in vitro fertilisation using pituitary suppression and human menopausal gonadotrophin Fertil Steril 48 1987 811 815
D. De Ziegler, M.I. Cedars, D. Randle, J.K.H. Lu, H.L. Judd, and D.R. Meldrum Suppression of the ovary using a gonadotrophin releasing-hormone agonist prior to stimulation for oocyte retrieval Fertil Steril 48 1987 807 810
R. Palermo, G. Amodeo, D. Navot, Z. Rosenwaks, and E. Cittadini Concomitant gonadotrophin-releasing hormone agonist and menotropin treatment for the synchronized induction of multiple follicles Fertil Steril 49 1988 290 295
R. De Roover, G. Genicot, S. Leonard, A. Massip, and F. Dessy Effect of an individually adapted superstimulation protocol for OPU in cows Anim Reprod Sci 2004 (in press)
L.F. Nasser, G.P. Adams, G.A. Bo, and R.J. Mapletoft Ovarian superstimulatory response relative to follicular wave emergence in heifers Theriogenology 40 1993 713 724
E.A. McGee, and J.W. Hsueh Initial and cyclic recruitment of ovarian follicles Endocr Rev 21 2000 200 214
J.E.J. Smitz, and R.G. Cortvrindt The earliest stages of folliculogenesis in vitro Reproduction 123 2002 185 202
A.N. Hirshfield Relationship between the supply of primordial follicles and the onset of follicular growth in rats Biol Reprod 50 1994 421 428
S.A. Wandji, V. Srsen, A.K. Voss, J.J. Eppig, and J.E. Fortune Initiation in vitro of growth of bovine primordial follicles Biol Reprod 55 1996 942 948
R.A. Cushman, J.C. DeSouza, V.S. Hedgpeth, and J.H. Britt Effect of long-term treatment with recombinant bovine somatotropin and oestradiol on hormone concentrations and ovulatory response of superovulated cattle Theriogenology 55 2001 1533 1547
Aerts J.M.J., Bols P.E.J., M. Oste, Van Ginneken C.J.D., E. Wechsung, and A. Houvenaghel Transvaginal ultrasound-guided biopsy of the bovine ovary: a new tool for the study of pre-antral follicles Reprod Fertil Dev 16 1-2 2004 229 230
