Reference : Assessment of nuclear totipotency of fetal bovine diploid germ cells by nuclear transfer.
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Assessment of nuclear totipotency of fetal bovine diploid germ cells by nuclear transfer.
Moens, A. [> > > >]
Chesne, P. [> > > >]
Delhaise, F. [> > > >]
Delval, A. [> > > >]
Ectors, Fabien mailto [Université de Liège - ULiège > > GIGA-Management : Plate-forme transgénique >]
Dessy, F. [> > > >]
Renard, Jeanne de Chantal [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
Heyman, Y. [> > > >]
Yes (verified by ORBi)
United States
[en] bovine embryo ; nuclear transfer ; germ cells
[en] Nuclear transfer was used to study nuclear reprogramming of fetal diploid bovine germ cells collected at two stages of the fetal development. In the first case, germ cells of both sexes were collected during their period of intragonadal mitotic multiplication at 48 days post coitum (d.p.c.). In the second case, only male germ cells were collected after this period, between 105 and 185 d.p.c. Isolated germ cells were fused with enucleated oocytes. Reconstituted embryos were cultured in vitro and those reaching the compacted morula or blastocyst stage were transferred into synchronous recipient heifers. Of 511 reconstituted embryos with 48 d.p.c. germ cells (309 males and 202 females), 48% (247/511 ) cleaved; 2.7% (14/511 ) reached the compacted morula stage and 8 of them the blastocyst stage (1.6%). No difference was observed between sexes. All 14 compacted morulae/blastocysts were transferred into 6 recipients and one pregnancy was initiated. This recipient was slaughtered at Day 35 and an abnormal conceptus (extended trophectoderm and degenerated embryo) was collected. Its male sex, genetically determined, corresponded to that of donor fetus. Of 380 reconstituted embryos with male 105 to 185 d.p.c. germ cells, 72.1% (274/380 ) cleaved, 2.1% (8 380 ) reached the compact morula stage and 7 of these the blastocyst stage (1.8%). Three blastocysts and one morula were transferred into 4 recipients. Two became pregnant at Day 21 but only one at Day 35 which aborted around Day 40. Our results show that the nucleus of diploid bovine germ cells of both sexes can be reprogrammed. However, in the absence of further development of these reconstituted embryos, nuclear totipotency of bovine diploid germ cells remains to be evidenced.
Researchers ; Professionals ; Students

File(s) associated to this reference

Fulltext file(s):

Open access
Moens et al_1996.pdfPublisher postprint778.6 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.