[en] The aim of this field study was to investigate the relationship of plasma urea nitrogen (PUN) with the pregnancy rate in lactating AwassixMerino ewes. One hundred and eighty-five AwassixMerino ewes were used in the present study. Ewes were fed a diet containing 17.4% crude protein and were milked twice a day by the milking machine. The ewes were synchronized for estrus by insertion of intravaginal sponges containing 30mg flurogestone acetate for 14 days. At the time of sponge removal each ewe was administered eCG (600IU). All ewes were inseminated twice with fresh semen into the external os of the cervix at 48 and 56h after sponge removal. The day of insemination was considered as Day 0 for calculating the gestational period. Blood samples were collected from each ewe at Days 0, 18 for measurement of PUN concentrations and at Day 22 after AI for measurement of pregnancy-associated glycoprotein (PAG) by radioimmunoassay (RIA). Thirty-eight ewes (20.5%) were confirmed pregnant by PAG-RIA test at Day 22 and by ultrasonography at Day 80. The mean (+/-S.D.) concentration of PUN in all ewes at Day 0 was 12.7+/-4.6mmol/L. There were non-significant differences in the level of PUN between pregnant and non-pregnant ewes at Days 0 (12.2+/-4.2mmol/L vs. 12.8+/-4.7mmol/L, respectively) and 18 (9.6+/-2.9mmol/L vs. 10.4+/-4.0mmol/L, respectively) after AI. Mean PUN concentrations decreased significantly from Day 0 to Day 18 after AI in both pregnant and non-pregnant ewes. By using logistic regression analysis, there was no effect of PUN concentrations on the probability of pregnancy occurrence in the studied ewes (odds ratio: 0.97; 95% confidence interval: 0.9-1.05; P=0.45). In conclusion, there was no evidence of a relationship between PUN concentration and pregnancy rate for lactating AwassixMerino ewes in the present study because of low pregnancy rate observed.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Karen, Aly; Kafrelsheikh University, Egypt > Department of Theriogenology, Faculty of Veterinary Medicine
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Bibliography
Acritopoulou-Fourcroy S., Pappas V., Peclaris G., Zervas N. Synchronization of estrus in ewes with Provera sponges/PMSG, prostaglandin F2 alpha, the prostaglandin analogue, ICI80996, and fertility following natural mating or artificial insemination. Reprod. Nutr. Dev. 1982, 22:345-354.
Berardinelli J.G., Weng J., Burfening P.J., Adair R. Effect of excess degradable intake protein on early embryonic development, ovarian steroids and blood urea nitrogen on days 2, 3, 4 and 5 of the estrous cycle in mature ewes. J. Anim. Sci. 2001, 79:193-199.
Bishonga C., Robinson J.J., Mc-Evoy T.G., Findlay P., Aitken R.P., Robetson I. Excess dietary urea intake in ewes and its effect on ovulation rate and embryo development. Jpn. J. Vet. Res. 1994, 44:139-151.
Butler W.R., Calaman J.J., Beam S.W. Plasma and milk urea nitrogen in relation to pregnancy rate in lactating dairy cattle. J. Anim. Sci. 1996, 74:858-865.
Canfield R.W., Sniffen C.J., Butler W.R. Effects of excess degradable protein on postpartum reproduction and energy balance in dairy cattle. J. Dairy Sci. 1990, 73:2342-2349.
Carroll D.J., Batron B.A., Anderson G.W., Smith R.D. Influence of protein intake and feeding strategy on reproductive performance of dairy cows. J. Dairy Sci. 1988, 71:3481-3740.
Elrod C.C., Butler W.R. Reduction of fertility and alteration of uterine pH in heifer fed excess ruminally degradable protein. J. Anim. Sci. 1993, 71:694-701.
Fahey J., Boland M.P., Callaghan D.C. The effect of dietary urea on embryo development in superovulated donor ewes and on early embryo survival and development in recipient ewes. Anim. Sci. 2001, 72:395-400.
Ferguson J.D., Galligan D.T., Blanchard T., Reeves M. Serum urea nitrogen and conception rate: the usefulness of test information. J. Dairy Sci. 1993, 76:3742-3746.
Garcia A., Neary M.K., Kelly G.R., Pierson R.A. Accuracy of ultrasonography in early pregnancy diagnosis in the ewe. Theriogenology 1993, 39:847-861.
Hammon D.S., Holyoak G.R., Dhiman T.R. Association between plasma urea nitrogen levels and reproductive fluid nitrogen and ammonia concentrations in early lactation dairy cows. Anim. Reprod. Sci. 2005, 86:195-204.
Hosmer D.W., Lemeshow S. Applied Logistic Regression 1989, John Wiley & Sons, New York.
Howard H.J., Aalseth E.P., Adam G.A., Bush L.J., McNew R.W., Dawson L.J. Influence of dietary protein on reproductive performance of dairy cows. J. Dairy Sci. 1987, 70:1563-1571.
Karen A., Beckers J.F., Sulon J., Sousa N.M., Szabados K., Reiczigel J., Szenci O. Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests. Theriogenology 2003, 59:1941-1948.
Kenny D.A., Boland M.G., Sreenan J.M. Effect of pasture crude protein and fermentable energy supplementation on blood metabolite and progesterone concentrations and embryo survival in heifers. Anim. Sci. 2001, 73:501-511.
Kenny D.A., Humpherson P.G., Lees H.J., Morris D.G., Tomos A.D., Diskin M.G., Sreenan J.M. Effect of elevated systemic concentrations of ammonia and urea on the metabolite and ionic composition of oviductal fluid in cattle. Biol. Reprod. 2002, 66:1797-1804.
Mc-Evoy T.G., Robinson J.J., Aitken R.P., Findlay P.A., Robetson I.S. Dietary excess of urea influence the viability and metabolism of preimplantation sheep embryos and may affect the fetal growth among survivors. Anim. Reprod. Sci. 1997, 47:71-90.
NRC Nutrient Requirements of Sheep 1985, National Academy Press, Washington, DC. 6th ed.
Orsokov E.R., Robinson J.J. The application of modern concepts of ruminant protein nutrition to sheep production system. Livestock Prod. Sci. 1981, 8:339-350.
Perényi Z., Szenci O., Sulon J., Drion P.V., Beckers J.F. Comparison of the ability of three radioimmunoassays to detect pregnancy-associated glycoproteins in bovine plasma. Reprod. Domest. Anim. 2002, 37:100-104.
Rajala-Schultz P.J., Saville W.J.A., Frazer G.S., Wittum T.E. Association between milk urea nitrogen and fertility in Ohio fairy cows. J. Dairy Sci. 2001, 84:482-489.
Rhoads M.L., Rhoads R.P., Gilbert R.O., Toole R., Butler W.R. Detrimental effects of high plasma urea nitrogen levels on viability of embryos from lactating dairy cows. Anim. Reprod. Sci. 2006, 91:1-10.
Shipely C.F.B., Buckrell B.C., Mylne M.J.A., Pollard M.J., Hunton J.R. Artificial insemination and embryo transfer in sheep. Current Therapy in Large Animal Theriogenology 2007, 629-641. Saunders Elsevier. 2nd edition. R.S. Youngquist, W.R. Threlfall (Eds.).
Talke H., Schubert G.E. Enzyme determination of urea in blood serum by the Warburg optical test. Klin. Wochenschr. 1965, 43:174-175.
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