Article (Scientific journals)
Human chondrocytes in tridimensional culture.
Bassleer, Corinne; Gysen, Philippe; Foidart, Jean-Michel et al.
1986In In Vitro Cellular and Developmental Biology: Journal of the Tissue Culture Association, 22, p. 113-9
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Keywords :
chondrocytes; suspension culture; cartilage; differentiation; tridimensional culture
Abstract :
[en] Cartilage was taken from the macroscopically normal part of human femoral heads immediately after orthopedic surgical operations for total prothesis consecutive to hip arthrosis. After clostridial collagenase digestion and repeated washings, chondrocytes (10(6) cells) were cultivated in a gyrotory shaker (100 rpm). Under these conditions, cells were kept in suspension and after 3 to 5 d formed a flaky aggregate which, on Day 10, became dense. These chondrocytes were morphologically differentiated: they had a round shape, were situated inside cavities, and were surrounded by a new matrix. Histochemical methods showed the presence of collagen and polysaccharides in cell cytoplasm and in intercellular matrix, and the immunofluorescence method using specific antisera (anticartilage proteoglycans and anti-type II collagen) showed that these two constituents were in intercellular matrix. The measurement of the amounts of proteoglycans (PG) released into culture medium and those present in chondrocyte aggregate (by a specific PG radioimmunoassay) showed a maximum production on Days 3 to 5 of culture, then the production decreased and stabilized (from Day 10 to the end of culture). The observed difference between the amounts of PG in aggregates after 20 d and those after 2 h of culture demonstrated that PG neosynthesis did occur during cultivation. This conclusion was supported by other results obtained by [14C]glucosamine incorporation in chondrocyte aggregates. Moreover, the aggregate fresh weight related to cell number (appreciated by DNA assay) increased significantly with culture duration. Three-dimensional chondrocyte culture represents an interesting model: chondrocytes were differentiated morphologically as well as biosynthetically and synthesized a new cartilage matrix.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bassleer, Corinne ;  Université de Liège - ULiège
Gysen, Philippe ;  Université de Liège - ULiège
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Bassleer, Roger ;  Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Franchimont, Paul ;  Université de Liège - ULiège
Language :
English
Title :
Human chondrocytes in tridimensional culture.
Publication date :
1986
Journal title :
In Vitro Cellular and Developmental Biology: Journal of the Tissue Culture Association
ISSN :
0883-8364
Publisher :
Tissue Culture Association, Gaithersburg, United States - Maryland
Volume :
22
Pages :
113-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 March 2011

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