Reference : Investigation of Epstein-Barr virus in Tunisian breast carcinomas
Scientific journals : Article
Human health sciences : Oncology
Investigation of Epstein-Barr virus in Tunisian breast carcinomas
Hachana, Mohamed Ridha [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Amara, Khaled [ > > ]
Ziadi, Sonia [ > > ]
Sriha, Badreddine [ > > ]
Korbi, Sadok [ > > ]
Trimeche, Mounir [ > > ]
Pathology - Research & Practice
Gustav Fischer Verlag
Yes (verified by ORBi)
[en] Breast cancer ; Epstein–Barr virus ; Tunisia
[en] Breast carcinoma is a major cause of death among women, and the potential implication of viruses in its pathogenesis remains worth a hypothesis. The potential role of Epstein-Barr virus (EBV) in its pathogenesis is still a subject of continued discussion and investigation. The aim of this study was to evaluate the prevalence of EBV in sporadic breast cancers in Tunisia, and to determine the clinicopathological characteristics of virus-positive cases. Viral presence has been evaluated by polymerase chain reaction (PCR), in situ hybridization, and immunohistochemistry investigated on tumor tissues and their corresponding normal breast tissues collected from 123 Tunisian women with sporadic breast carcinomas. Viral status in tumors was then correlated with various clinicopathological parameters. Using specific PCR assays, EBV DNA was found in 33 (27%) out of 123 breast carcinoma cases. EBV-encoded small RNAs (EBERs) in situ hybridization was negative in the neoplastic cells, but stomal lymphocytes were positive in 4 cases. Immunohistochemistry for latent membrane protein 1 (LMP1) was negative in all cases. None of the normal breast tissues showed positive results for EBV using PCR, in situ hybridization, and immunohistochemistry. A correlation was found between EBV DNA presence and the negativity of estrogen receptor (P=0.008). However, no significant correlation was found for the other parameters investigated, including patient age, Scarff-Bloom-Richardson (SBR) histological grade, tumor size, and histological node involvement. With regard to survival data, overall and disease-free survivals were shorter in EBV-positive breast carcinoma cases than in EBV-negative ones, but this difference did not reach statistical significance. Our study indicates the presence of EBV DNA in a significant proportion of breast cancer in Tunisia. Further studies are required to elucidate the role of this virus in breast carcinogenesis.
Researchers ; Professionals

File(s) associated to this reference

Fulltext file(s):

Restricted access
science-4.pdfPublisher postprint1.18 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.