Correlation of five radioimmunoassay systems for measurement of bovine plasma pregnancy-associated glycoprotein concentrations at early pregnancy period.
Ayad, A.; Melo de Sousa, Noelita; Sulon, Josephet al.
2009 • In Research in Veterinary Science, 86, p. 377-382
[en] The measurement of serum or plasma PAG concentrations is currently used as a specific method for pregnancy diagnosis in cattle. In this study, the correlation between five radioimmunoassay systems (RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool) developed for measurement of PAG concentrations in ruminant species was investigated in plasma from pregnant Friesian Holstein females. Plasma PAG concentrations (ng/mL) measured by different RIA systems were significantly correlated between them ( 0.81; P<0.001). PAG concentrations increased significantly from Day 21 (n=27) to 30 (n=37) after AI by use of all PAG-RIA systems. From Day 30 to 80 after AI, lower PAG concentrations were observed when using the homologous system RIA-497. The addition of several proteinase inhibitors changed neither the non specific binding nor the B(0) binding to the tracer. Our results suggest that all tested PAG-RIA (RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool) are highly correlated and can be useful to follow PAG concentrations in samples collected during the first trimester of gestation.
Research Center/Unit :
Laboratory of Endocrinology and Animal Reproduction
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ayad, A.; University Abderahmane Mira, 06000 Bejaia, Algeria > Organism and Populations Biology, Faculty of Life and Nature Sciences
Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Sulon, Joseph ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Hornick, Jean-Luc ; Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Iguer-Ouada, M.; University Abderahmane Mira, 06000 Bejaia, Algeria > Organism and Populations Biology, Faculty of Life and Nature Sciences
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Correlation of five radioimmunoassay systems for measurement of bovine plasma pregnancy-associated glycoprotein concentrations at early pregnancy period.
Publication date :
2009
Journal title :
Research in Veterinary Science
ISSN :
0034-5288
eISSN :
1532-2661
Publisher :
British Veterinary Association, London, United Kingdom
Volume :
86
Pages :
377-382
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique Walloon region
Funding number :
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS (Grant No. CC 1.5.059.08); Ministry of the Wallonne Region-DGA (Grant No. D31-1184)
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