[en] Blood oxygen transport and tissue oxygenation were studied in 28 calves from the Belgian White and Blue breed (20 healthy and 8 hypoxaemic ones). Hypoxaemic calves were selected according to their high respiratory frequency and to their low partial oxygen pressure (PaO2) in the arterial blood. Venous and arterial blood samples were collected, and 2,3-diphosphoglycerate, adenosine triphosphate, chloride, inorganic phosphate and hemoglobin concentrations, and pH, PCO, and PO2 were determined. An oxygen equilibrium curve (OEC) was measured in standard conditions, for each animal. The arterial and venous OEC were calculated, taking body temperature, pH and PCO2 values in arterial and venous blood into account. The oxygen exchange fraction (OEF%), corresponding to the degree of blood desaturation between the arterial and the venous compartments, and the amount of oxygen released at the tissue level by 100 mL of blood (OEF Vol%) were calculated from the arterial and venous OEC combined with the PO2 and hemoglobin concentration. In hypoxaemic calves investigated in this study, the hemoglobin oxygen affinity, measured under standard conditions, was not modified. On the contrary, in vivo acidosis and hypercapnia induced a decrease in the hemoglobin oxygen affinity in arterial blood, which combined to the decrease in PaO2 led to a reduced hemoglobin saturation degree in the arterial compartment. However, this did not impair the oxygen exchange fraction (OEF%), since the hemoglobin saturation degree in venous blood was also diminished.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Cambier, Carole ; Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Clerbaux, Thierry
Detry, Bruno; Cliniques Universitaires Saint-Luc - UCL > Département de Médecine interne > Pneumologie
Marville, Vincent
Frans, Albert
Gustin, Pascal ; Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Alnoor S.A., Slocombe R.F., Derksen F.J., Robinson N.E. (1986) Hemodynamic effects of acute pneumonia experimentally induced in newborn calves inoculated with Pasteurella haemolytica. Am. J. Vet. Res 47:1382-1386.
Amory H., Rollin F., Desmecht D., Linden A., Lekeux P. (1992) Cardiovascular response to acute hypoxia in double-muscled calves. Res. Vet. Sci 52:316-324.
Bossi D., Giardina B. (1996) Red cell Physiology. Mol. Aspects Med 17:117-128.
Cambier C. (2001) Transport de l'oxygène par le sang et oxygénation tissulaire chez le bovin en conditions physiologiques et pathologiques. Modulation pharmacologique de ces fonctions chez le veau sain., (Ph.D. thesis). Université de Liège, Faculté de Médecine Vétérinaire; .
Cambier C., Detry B., Beerens D., Florquin S., Ansay M., Frans A., Clerbaux T., Gustin P. (1998) Effects of hyperchloremia on blood oxygen binding in healthy calves. J. Appl. Physiol 85:1267-1272.
Cambier C., Clerbaux T., Detry B., Beerens D., Frans A., Gustin P. (2000) Blood oxygen binding in double-muscled calves and dairy calves with conventional muscle conformation. Am. J. Vet. Res 61:299-304.
Cambier C., Clerbaux Th., Moreaux B., Detry B., Beerens D., Frans A., Gustin P. (2001) Blood oxygen binding in calves with naturally occurring diarrhea. Am. J. Vet. Res 62:799-804.
Clerbaux Th., Fesler R., Bourgeois J. (1973) A dynamic method for continuous recording of the whole blood oxyhemoglobin dissociation curve at constant temperature, pH and PCO2. Med. Lab. Technol 30:1-9.
Clerbaux Th., Reynaert M., Willems E., Frans A. (1989) Effect of phosphate on oxygen-hemoglobin affinity, diphosphoglycerate and blood gases recovery from diabetic ketoacidosis. Intensive Care Med 15:495-498.
Clerbaux Th., Gustin P., Detry B., Cao M.L., Frans A. (1993) Comparative study of the oxyhaemoglobin dissociation curve of four mammals: Man, dogs, horse and cattle. Biochem. Physiol. A 106:687-694.
Clerbaux Th., Detry B., Reynaert M., Frans A. (1997) Right shift of the oxyhemoglobin dissociation curve in acute respiratory distress syndrome. Pathol. Biol 45:269-273.
Constable P.D., Schmall L.M., Muir W.W., Hoffsis G.F. (1991) Respiratory, renal, hematologic, and serum biochemical effects of hypertonic saline solution in endotoxemic calves. Am. J. Vet. Res 52:990-998.
de Morais A.H.S. (1982) Chloride ion in small animal practice: The forgotten ion. Veterinary Emergency and Critical Care 2:11-24.
Eigenmann U.J.E., Schoon H.A., Janh D., Grunert E. (1984) Neonatal respiratory distress syndrome in the calf. Vet. Rec 114:141-144.
Fronticelli C. (1990) A possible new mechanism of oxygen affinity modulation in mammalian hemoglobins. Biophys. Chem 37:141-146.
Gustin P., Clerbaux Th., Willems E., Lekeux P., Lomba F., Frans A. (1988) Oxygen transport properties of blood in two different bovine breeds. Comp. Biochem. Physiol. A 89:553-558.
Gustin P., Detry B., Cao M.L., Chenut F., Robert A., Ansay M., Frans A., Clerbaux Th. (1994) Chloride and inorganic phosphate modulate binding of oxygen bovine red blood cells. J. Appl. Physiol 77:202-208.
Gustin P., Detry B., Robert A., Cao M.L., Lessire F., Cambier C., Katz V., Ansay M., Frans A., Clerbaux Th. (1997) Influence of age and breed on the binding of oxygen to red blood cells of bovine calves. J. Appl. Physiol 82:784-790.
Lekeux P., Rollin F., Art T. (1993) Control of breathing in resting and exercising animals. Pulmonary Function in Healthy, Exercising and Diseased Animals , Simoens P. (Ed.), University of Ghent; 123-143.
Maillard D., Fleury B., Housset B., Laffont S., Chabolle J., Derenne Ph. (1991) Decreased oxyhemoglobin affinity in patients with sleep apnea syndrome. Am. Rev. Respir. Dis 143:486-489.
Manfredi F., Passo T., Atkinson K. (1971) Blood 2,3 diphosphoglycerate level (DPG) in chronic obstructive airway disease. Clin. Res 19:683.
Martin L. (1993) SaO2 et concentration en oxygène. L'essentiel sur l'interprétation des gaz du sang artériel , Martin L. (Ed.), Edisem Maloine, Canada; 69-92.
Matsumoto Y. (1995) The function of red blood cells during experimental hemorrhagic and endotoxic shock. Masui 44:342-348.
Murphy E.M., Bone R.C., Hiller F.C., Diederich D.A., Ruth W.E. (1978) The oxyhemoglobin dissociation curve in type A and type B COPD. Lung 154:299-305.
Perutz M.F., Fermi G., Poyart C., Pagnier J., Kister J. (1993) A novel allosteric mechanism in haemoglobin. Structure of bovine deoxyhaemoglobin, absence of specific chloride-binding sites and origin of the chloride-linked Bohr effect in bovine and human haemoglobin. J. Mol. Biol 233:536-545.
Samaja M. (1997) Blood gas transport at high altitude. Respiration 64:422-428.
Schmitz T., Bair N., Falk M., Levine C. (1995) A comparison of five methods of temperature measurement in febrile intensive care patients. Am. J. Crit. Care 4:286-292.
Tomasic M. (1999) Temporal changes in core body temperature in anesthetized adult horses. Am. J. Vet. Res 60:556-562.
Vestweber J.G. (1997) Respiratory problems of newborn calves. Vet. Clin. North Am. Food Anim. Pract 13:411-423.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.