Reference : Effect of sampling method and incubation temperature on fungal culture in canine sino...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/8524
Effect of sampling method and incubation temperature on fungal culture in canine sino-nasal aspergillosis
English
Billen, Frédéric mailto [Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux >]
Clercx, Cécile mailto [Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux >]
Le Garérrès, Alain [> >]
Massart, Laurent [> >]
Mignon, Bernard mailto [> >]
Peeters, Dominique mailto [Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Médecine interne des animaux de compagnie >]
2009
Journal of Small Animal Practice
Blackwell Publishing
50
2
67-72
Yes (verified by ORBi)
International
0022-4510
1748-5827
Oxford
United Kingdom
[en] sino-nasal aspergillosis ; fungal culture ; incubation temperature ; dog ; Aspergillus fumigatus
[en] OBJECTIVES: To evaluate the most appropriate sampling procedure and the effect of incubation temperature on fungal culture in the diagnosis of canine sinonasal aspergillosis (SNA). METHODS: Sixteen dogs with SNA and 20 dogs with non-fungal nasal disease entered a prospective study. Nasal secretions and mucosal biopsies were collected in all dogs. Fungal plaques were also sampled in dogs with SNA. Each specimen was taken in duplicate from each dog and incubated at room temperature and 37 degrees C. RESULTS: In dogs with SNA, nasal secretions, mucosal biopsies and fungal plaques yielded fungal growth at room temperature in one, one and seven dogs, respectively, whereas fungal growth was obtained at 37 degrees C in three, 12 and 14 dogs, respectively. No specimen collected from any dog with non-fungal nasal disease yielded fungal growth at room temperature or at 37 degrees C. CLINICAL SIGNIFICANCE: The diagnosis of canine SNA is more likely to be confirmed following culture of mucosal biopsies or fungal plaques than nasal secretions sampled blindly with swabs. Incubating cultures at 37 degrees C is more likely to provide a diagnostic outcome than when samples are cultured at room temperature. Fungal culture of nasal specimens has good specificity for the diagnosis of SNA in dogs.
Researchers ; Professionals
http://hdl.handle.net/2268/8524
also: http://hdl.handle.net/2268/8362
10.1111/j.1748-5827.2008.00672.x

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