[en] Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, EL-18 and TNF-alpha relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-beta, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously. (c) 2005 Elsevier B.V. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Peeters, Dominique ; Université de Liège - ULiège > Clinique des petits animaux
Peters, I. R.
Clercx, Cécile ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Day, M. J.
Language :
English
Title :
Quantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Blease K., Mehrad B., Lukacs N.W., Kunkel S.L., Standiford T.J., and Hogaboam C.M. Antifungal and airway remodeling roles for murine monocyte chemoattractant protein-1/CCL2 during pulmonary exposure to Aspergillus fumigatus conidia. J. Immunol. 166 (2001) 1832-1842
Borger P., Koeter G.H., Timmerman J.A., Vellenga E., Tomee J.F., and Kauffman H.F. Proteases from Aspergillus fumigatus induce interleukin (IL)-6 and IL-8 production in airway epithelial cell lines by transcriptional mechanisms. J. Infect. Dis. 180 (1999) 1267-1274
Brieland J.K., Jackson C., Menzel F., Loebenberg D., Cacciapuoti A., Halpern J., Hurst S., Muchamuel T., Debets R., Kastelein R., Churakova T., Abrams J., Hare R., and O'Garra A. Cytokine networking in lungs of immunocompetent mice in response to inhaled Aspergillus fumigatus. Infect. Immun. 69 (2001) 1554-1560
Cenci E., Mencacci A., Fe d'Ostiani C., Del Sero G., Mosci P., Montagnoli C., Bacci A., and Romani L. Cytokine- and T helper-dependent lung mucosal immunity in mice with invasive pulmonary aspergillosis. J. Infect. Dis. 178 (1998) 1750-1760
Cenci E., Perito S., Enssle K.H., Mosci P., Latge J.P., Romani L., and Bistoni F. Th1 and Th2 cytokines in mice with invasive aspergillosis. Infect. Immun. 65 (1997) 564-570
Hamilos D.L., and Lund V.J. Etiology of chronic rhinosinusitis: the role of fungus. Ann Otol. Rhinol. Laryngol. 193 Suppl. (2004) 27-31
Hernandez Y., Arora S., Erb-Downward J.R., McDonald R.A., Toews G.B., and Huffnagle G.B. Distinct roles for IL-4 and IL-10 in regulating T2 immunity during allergic bronchopulmonary mycosis. J. Immunol. 174 (2005) 1027-1036
Hori S., Carvalho T.L., and Demengeot J. CD25+CD4+ regulatory T cells suppress CD4+ T cell-mediated pulmonary hyperinflammation driven by Pneumocystis carinii in immunodeficient mice. Eur. J. Immunol. 32 (2002) 1282-1291
Kohka H., Yoshino T., Iwagaki H., Sakuma I., Tanimoto T., Matsuo Y., Kurimoto M., Orita K., Akagi T., and Tanaka N. Interleukin-18/interferon-gamma-inducing factor, a novel cytokine, up-regulates ICAM-1 (CD54) expression in KG-1 cells. J. Leukoc. Biol. 64 (1998) 519-527
Mathews K. Fungal rhinitis. In: King L. (Ed). Textbook of Respiratory Disease in Dogs and Cats (2004), Saunders, St Louis 284-293
Mehrad B., Strieter R.M., and Standiford T.J. Role of TNF-alpha in pulmonary host defense in murine invasive aspergillosis. J. Immunol. 162 (1999) 1633-1640
Morrison B.E., Park S.J., Mooney J.M., and Mehrad B. Chemokine-mediated recruitment of NK cells is a critical host defense mechanism in invasive aspergillosis. J. Clin. Invest. 112 (2003) 1862-1870
Nagai H., Guo J., Choi H., and Kurup V. Interferon-gamma and tumor necrosis factor-alpha protect mice from invasive aspergillosis. J. Infect. Dis. 172 (1995) 1554-1560
Peeters D., Day M.J., and Clercx C. An immunohistochemical study of canine nasal aspergillosis. J. Comp. Pathol. 132 (2005) 283-288
Peeters D., Peters I.R., Farnir F., Clercx C., and Day M.J. Real-time RT-PCR quantification of mRNA encoding cytokines and chemokines in histologically normal canine nasal, bronchial and pulmonary tissue. Vet. Immunol. Immunopathol. 104 (2005) 195-204
Peeters, D., Peters, I.R., Clercx, C., Day, M.J., 2006. Real-time RT-PCR quantification of mRNA encoding cytokines, CC chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneumopathy. Vet. Immunol. Immunopathol., in press.
Peters I.R., Helps C.R., Calvert E.L., Hall E.J., and Day M.J. Cytokine mRNA quantification in histologically normal canine duodenal mucosa by real-time RT-PCR. Vet. Immunol. Immunopathol. 103 (2005) 101-111
Peters I.R., Helps C.R., Calvert E.L., Hall E.J., and Day M.J. Measurement of mRNA encoding the [alpha]-chain, polymeric immunoglobulin receptor, and J-chain in duodenal mucosa from dogs with and without chronic diarrhea by use of quantitative real-time reverse transcription polymerase chain reaction assays. Am. J. Vet. Res. 66 (2005) 11-16
Peters I.R., Helps C.R., Hall E.J., and Day M.J. Real-time RT-PCR: considerations for efficient and sensitive assay design. J. Immunol. Methods 286 (2004) 203-217
Pylkkanen L., Gullsten H., Majuri M.L., Andersson U., Vanhala E., Maatta J., Meklin T., Hirvonen M.R., Alenius H., and Savolainen K. Exposure to Aspergillus fumigatus spores induces chemokine expression in mouse macrophages. Toxicology 200 (2004) 255-263
Richardson M.D., and Patel M. Stimulation of neutrophil phagocytosis of Aspergillus fumigatus conidia by interleukin-8 and N-formylmethionyl-leucylphenylalanine. J. Med. Vet. Mycol. 33 (1995) 99-104
Roilides E., Dimitriadou A., Kadiltsoglou I., Sein T., Karpouzas J., Pizzo P.A., and Walsh T.J. IL-10 exerts suppressive and enhancing effects on antifungal activity of mononuclear phagocytes against Aspergillus fumigatus. J. Immunol. 158 (1997) 322-329
Roilides E., Dimitriadou-Georgiadou A., Sein T., Kadiltsoglou I., and Walsh T.J. Tumor necrosis factor alpha enhances antifungal activities of polymorphonuclear and mononuclear phagocytes against Aspergillus fumigatus. Infect. Immun. 66 (1998) 5999-6003
Romani L. Immunity to fungal infections. Nat. Rev. Immunol. 4 (2004) 1-23
Saunders J.H., Clercx C., Snaps F.R., Sullivan M., vanBree H., and Dondelinger R.F. Radiographic, magnetic resonance, computed tomographic and rhinoscopic features of canine nasal aspergillosis. J. Am. Vet. Med. Assoc. 225 (2004) 1703-1712
Saunders J.H., Zonderland J.L., Clercx C., Gielen I., Snaps F.R., Sullivan M., vanBree H., and Dondelinger R.F. Computed tomographic findings in 35 dogs with nasal aspergillosis. Vet. Radiol. Ultrasound 43 (2002) 5-9
Sharp N.J.H., Harvey C.E., and Sullivan M. Canine nasal aspergillosis and penicilliosis. Comp. Cont. Educ. 13 (1991) 41
Tomee J.F., Wierenga A.T., Hiemstra P.S., and Kauffman H.K. Proteases from Aspergillus fumigatus induce release of proinflammatory cytokines and cell detachment in airway epithelial cell lines. J. Infect. Dis. 176 (1997) 300-303
Uri N., Cohen-Kerem R., Elmalah I., Doweck I., and Greenberg E. Classification of fungal sinusitis in immunocompetent patients. Otolaryngol. Head Neck Surg. 129 (2003) 372-378
Zonderland J.L., Stork C.K., Saunders J.H., Hamaide A.J., Balligand M.H., and Clercx C.M. Intranasal infusion of enilconazole for treatment of sinonasal aspergillosis in dogs. J. Am. Vet. Med. Assoc. 221 (2002) 1421-1425
