[en] Increased levels of Mcl-1 (myeloid cell factor-1) have
been reported in several cancers, suggesting an important
role played by Mcl-1 in cancer cell survival. Mcl-1 is
an anti-apoptotic protein shown to delay or block apoptosis.
In this work, using semiquantitative immunofluorescence,
real-time PCR, and RNase protection assay,
an increase in Mcl-1 expression was detected in hepatoma
HepG2 cells incubated under hypoxia or in the
presence of cobalt chloride. Through analysis of the
Mcl-1 promoter sequence, a putative HIF-1 (hypoxiainducible
factor-1) binding site was identified. A Mcl-1
promoter fragment containing this hypoxia-responsive
element was able to bind HIF-1 in vitro. It also induced
hypoxia-dependent transcription of a luciferase reporter
gene, which was suppressed by anti-HIF-1 short
interfering RNA. Finally, overexpression of Mcl-1 protected
HepG2 cells against apoptosis induced by tertbutyl
hydroperoxide as shown by inhibition of caspase-3
activation and DNA fragmentation. All these data suggest
a potential anti-apoptotic role of HIF-1 that could
protect cells against apoptosis under hypoxia by overexpression
of the Mcl-1 protein.
Research center :
Unité de Recherche en Biologie Cellulaire
Disciplines :
Oncology
Author, co-author :
Piret, Jean-Pascal
Minet, Emmanuel
Cosse, Jean-Philippe ; Université de Liège - ULiège > GIGA-R : Epigénétique Cellulaire et Moléculaire
Ninane, Noelle
Debacq, Christophe
Raes, Martine
Michiels, Carine
Language :
English
Title :
Hypoxia-inducible Factor-1-dependent Overexpression of Myeloid Cell Factor-1 Protects Hypoxic Cells against tert-Butyl Hydroperoxide-induced Apoptosis
Publication date :
March 2005
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, Baltimore, United States - Maryland
Volume :
280
Pages :
9336
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FUNDP - Facultés Universitaires Notre-Dame de la Paix [BE]
Ivan, M., Kondo, K., Yang, H., Kim, W., Valiando, J., Ohh, M., Salic, A., Asara, J. M., Lane, W. S., and Kaelin, W. G., Jr. (2001) Science 292, 464-468
Jeong, J. W., Bae, M. K., Ahn, M. Y., Kim, S. H., Sohn, T. K., Bae, M. H., Yoo, M. A., Song, E. J., Lee, K. J., and Kim, K. W. (2002) Cell 111, 709-720
Jaakkola, P., Mole, D. R., Tian, Y. M., Wilson, M. I., Gielbert, J., Gaskell, S. J., Kriegsheim, A., Hebestreit, H. F., Mukherji, M., Schofield, C. J., Maxwell, P. H., Pugh, C. W., and Ratcliffe, P. J. (2001) Science 292, 468-472
Lando, D., Peet, D. J., Gorman, J. J., Whelan, D. A., Whitelaw, M. L., and Bruick, R. K. (2002) Genes Dev. 16, 1466-1471
Carmeliet, P., Dor, Y., Herbert, J. M., Fukumura, D., Brusselmans, K., Dewerchin, M., Neeman, M., Bono, F., Abramovitch, R., Maxwell, P., Koch, C. J., Ratcliffe, P., Moons, L., Jain, R. K., Collen, D., Keshert, E., and Keshet, E. (1998) Nature 394, 485-490
Blagosklonny, M. V., An, W. G., Romanova, L. Y., Trepel, J., Fojo, T., and Neckers, L. (1998) J. Biol. Chem. 273, 11995-11998
An, W. G., Kanekal, M., Simon, M. C., Maltepe, E., Blagosklonny, M. V., and Neckers, L. M. (1998) Nature 392, 405-408
Sowter, H. M., Ratcliffe, P. J., Watson, P., Greenberg, A. H., and Harris, A. L. (2001) Cancer Res. 61, 6669-6673
Bruick, R. K. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 9082-9087
Kim, J. Y., Ahn, H. J., Ryu, J. H., Suk, K., and Park, J. H. (2004) J. Exp. Med. 199, 113-124
Shoshani, T., Faerman, A., Mett, I., Zelin, E., Tenne, T., Gorodin, S., Moshel, Y., Elbaz, S., Budanov, A., Chajut, A., Kalinski, H., Kamer, I., Rozen, A., Mor, O., Keshet, E., Leshkowitz, D., Einat, P., Skaliter, R., and Feinstein, E. (2002) Mol. Cell. Biol. 22, 2283-2293
Baek, J. H., Jang, J. E., Kang, C. M., Chung, H. Y., Kim, N. D., and Kim, K. W. (2000) Oncogene 19, 4621-4631
Alvarez-Tejado, M., Naranjo-Suàrez, S., Jiménez, C., Carrera, A. C., Landàzuri, M. O., and del Peso, L. (2001) J. Biol. Chem. 276, 22368-22374
Dong, Z., Venkatachalam, M. A., Wang, J., Patel, Y., Saikumar, P., Semenza, G. L., Force, T., and Nishiyama, J. (2001) J. Biol. Chem. 276, 18702-18709
Erler, J. T., Cawthorne, C. J., Williams, K. J., Koritzinsky, M., Wouters, B. G., Wilson, C., Miller, C., Demonacos, C., Stratford, I. J., and Dive, C. (2004) Mol. Cell. Biol. 24, 2875-2889
Dong, Z., and Wang, J. (2004) J. Biol. Chem. 279, 9215-9221
Piret, J. P., Lecocq, C., Toffoli, S., Ninane, N., Raes, M., and Michiels, C. (2004) Exp. Cell Res. 295, 340-349
Akakura, N., Kobayashi, M., Horiuchi, I., Suzuki, A., Wang, J., Chen, J., Niizeki, H., Kawamura, K., Hosokawa, M., and Asaka, M. (2001) Cancer Res. 61, 6548-6554
Yu, E. Z., Li, Y. Y., Liu, X. H., Kagan, E., and McCarron, R. M. (2004) Lab. Investig. 84, 553-561
Piret, J. P., Mottet, D., Raes, M., and Michiels, C. (2002) Biochem. Pharmacol. 64, 889-892
Leuenroth, S. J., Grutkoski, P. S., Ayala, A., and Simms, H. H. (2000) J. Leukocyte Biol. 68, 158-166
Leuenroth, S. J., Grutkoski, P. S., Ayala, A., and Simms, H. H. (2000) Surgery 128, 171-177
Kozopas, K. M., Yang, T., Buchan, H. L., Zhou, P., and Craig, R. W. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 3516-3520
Reynolds, J. E., Yang, T., Qian, L., Jenkinson, J. D., Zhou, P., Eastman, A., and Craig, R. W. (1994) Cancer Res. 54, 6348-6352
Zhou, P., Qian, L., Kozopas, K. M., and Craig, R. W. (1997) Blood 89, 630-643
Henson, E. S., Gibson, E. M., Villanueva, J., Bristow, N. A., Haney, N., and Gibson, S. B. (2003) J. Cell. Biochem. 89, 1177-1192
Wang, X., and Studzinski, G. P. (1997) Exp. Cell Res. 235, 210-217
Sato, T., Hanada, M., Bodrug, S., Irie, S., Iwama, N., Boise, L. H., Thompson, C. B., Golemis, E., Fong, L., Wang, H. G., and Reed, J. C. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 9238-9242
Leu, J. I., Dumont, P., Hafey, M., Murphy, M. E., and George, D. L. (2004) Nat. Cell Biol. 6, 443-450
Cuconati, A., Mukherjee, C., Perez, D., and White, E. (2003) Genes Dev. 17, 2922-2932
Krajewska, M., Krajewski, S., Epstein, J. I., Shabaik, A., Sauvageot, J., Song, K., Kitada, S., and Reed, J. C. (1996) Am. J. Pathol. 148, 1567-1576
Derenne, S., Monia, B., Dean, N. M., Taylor, J. K., Rapp, M. J., Harousseau, J. L., Bataille, R., and Amiot, M. (2002) Blood 100, 194-199
Rassidakis, G. Z., Lai, R., McDonnell, T. J., Cabanillas, F., Sarris, A. H., and Medeiros, L. J. (2002) Am. J. Pathol. 160, 2309-2310
Kitada, S., Andersen, J., Akar, S., Zapata, J. M., Takayama, S., Krajewski, S., Wang, H. G., Zhang, X., Bullrich, F., Croce, C. M., Rai, K., Hines, J., and Reed, J. C. (1998) Blood 91, 3379-3389
Zhang, B., Gojo, I., and Fenton, R. G. (2002) Blood 99, 1885-1893
Epling-Burnette, P. K., Liu, J. H., Catlett-Falcone, R., Turkson, J., Oshiro, M., Kothapalli, R., Li, Y., Wang, J. M., Yang-Yen, H. F., Karras, J., Jove, R., and Loughran, T. P., Jr. (2001) J. Clin. Investig. 107, 351-362
Renard, P., Ernest, I., Houbion, A., Art, M., Le Calvez, H., Raes, M., and Remacle, J. (2001) Nucleic Acids Res. 29, E21
Semenza, G. L., and Wang, G. L. (1992) Mol. Cell. Biol. 12, 5447-5454
Akgul, C., Turner, P. C., White, M. R., and Edwards, S. W. (2000) Cell Mol. Life Sci, 57, 684-691
Ebert, B. L., Firth, J. D., and Ratcliffe, P. J. (1995) J. Biol. Chem. 270, 29083-29089
Wang, J. M., Chao, J. R., Chen, W., Kuo, M. L., Yen, J. J., and Yang-Yen, H. F. (1999) Mol. Cell. Biol. 19, 6195-6206
Townsend, K. J., Zhou, P., Qian, L., Bieszczad, C. K., Lowrey, C. H., Yen, A., and Craig, R. W. (1999) J. Biol. Chem. 274, 1801-1813
Harris, A. L. (2002) Nat. Rev. Cancer 2, 38-47
Brown, J. M. (1999) Cancer Res. 59, 5863-5870
Zagzag, D., Zhong, H., Scalzitti, J. M., Laughner, E., Simons, J. W., and Semenza, G. L. (2000) Cancer 88, 2606-2618
Zhong, H., De Marzo, A. M., Laughner, E., Lim, M., Hilton, D. A., Zagzag, D., Buechler, P., Isaacs, W. B., Semenza, G. L., and Simons, J. W. (1999) Cancer Res. 59, 5830-5835
Zhong, H., Agani, F., Baccala, A. A., Laughner, E., Rioseco-Camacho, N., Isaacs, W. B., Simons, J. W., and Semenza, G. L. (1998) Cancer Res. 58, 5280-5284
Rofstad, E. K., Sundfor, K., Lyng, H., and Trope, C. G. (2000) Br. J. Cancer 83, 354-359
Hockel, M., Schlenger, K., Aral, B., Mitze, M., Schaffer, U., and Vaupel, P. (1996) Cancer Res. 56, 4509-4515
Ryan, H. E., Lo, J., and Johnson, R. S. (1998) EMBO J. 17, 3005-3015
Ryan, H. E., Poloni, M., McNulty, W., Elson, D., Gassmann, M., Arbeit, J. M., and Johnson, R. S. (2000) Cancer Res. 60, 4010-4015
Seagroves, T. N., Ryan, H. E., Lu, H., Wouters, B. G., Knapp, M., Thibault, P., Laderoute, K., and Johnson, R. S. (2001) Mol. Cell. Biol. 21, 3436-3444
Feldser, D., Agani, F., Iyer, N. V., Pak, B., Ferreira, G., and Semenza, G. L. (1999) Cancer Res. 59, 3915-3918
Krishnamachary, B., Berg-Dixon, S., Kelly, B., Agani, F., Feldser, D., Ferreira, G., Iyer, N., LaRusch, J., Pak, B., Taghavi, P., and Semenza, G. L. (2003) Cancer Res. 63, 1138-1143
Lin, Z., Weinberg, J. M., Malhotra, R., Merritt, S. E., Holzman, L. B., and Brosius, F. C., III (2000) Am. J. Physiol. 278, E958-E966
Chen, J., Zhao, S., Nakada, K., Kuge, Y., Tamaki, N., Okada, F., Wang, J., Shindo, M., Higashino, F., Takeda, K., Asaka, M., Katoh, H., Sugiyama, T., Hosokawa, M., and Kobayashi, M. (2003) Am. J. Pathol 162, 1283-1291
Moulding, D. A., Giles, R. V., Spiller, D. G., White, M. R., Tidd, D. M., and Edwards, S. W. (2000) Blood 96, 1756-1763