[en] Twenty colonic adenocarcinomas were studied by immunofluorescence with antisera against components of the basement membrane: type IV collagen, laminin and heparan sulfate-rich proteoglycan, as well as antisera against antigens of the connective tissue: type-III collagen, fibronectin and hyaluronectin. Marked alterations of the basement membranes were consistently observed on staining with each one of the first three antisera. In contrast, staining of the normal components of connective tissue was in most cases as intense as in normal colonic mucosa. Hyaluronectin, a marker of peritumoral stroma, was found to be present in 12 out 15 tumors studied.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Burtin, P.
Chavanel, G.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Martin, E.
Language :
English
Title :
Antigens of the basement membrane and the peritumoral stroma in human colonic adenocarcinomas: an immunofluorescence study.
Publication date :
1982
Journal title :
International Journal of Cancer
ISSN :
0020-7136
eISSN :
1097-0215
Publisher :
Wiley Liss, Inc., New York, United States - New York
Albrechtsen R., Nielson M., Wewer U., Engvall E., Ruoslahti E. (1981) Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin. Cancer Res. 41:5076-5081.
Birembaut, Gaillard D., Adnet J.J., Dousset H., Kalis B., Poynard J.P., Leutenegger M., Labat‐Robert J., Robert L. (1981) La fibronectine. Etude immuno‐morphologique de sa distribution en pathologie. Ann. Pathol. 1:140-146.
Delpech B., Delpech A., Girard N. (1979) An antigen associated with mesenchyme in human tumors that cross‐reacts with brain glycoprotein. Brit, J. Cancer 40:123-133.
Delpech B., Halavent C. (1981) Characterization and purification from human brain of a hyaluronic acid‐binding glycoprotein, hyaluronectin. J. Neurochem. 36:855-859.
Foidart J.M., Bere E.W., Yaar M., Rennard S.I., Gullino M., Martin G.R., Katz S.I. (1980) Distribution and immunoelectron microscopic localization of laminin, a non collagenous basement membrane glycoprotein. Lab. Invest. 42:336-342.
Foidart J.M., Reddi A.H. (1980) Immunofluorescent localization of type IV collagen and laminin during endochondral bone differentiation and regulation by pituitary growth hormone. Dev. Biol. 75:130-136.
Foidart J.M., Yaar M. (1981) Type IV collagen, laminin and fibronectin at the dermoepidermal junction. Frontiers of matrix biology. Epidermal keratinocytes differentiation and fibrillogenesis , M. Prunieras, Karger, Basel; 9:175-188.
Hassell J.R., Gehron‐Robey P., Barrach H.J., Wilczek J., Rennard S.I., Martin G.R. (1980) Isolation of a heparan sulfate‐containing proteoglycan from basement membrane. Proc. nat. Acad. Sci. (Wash.) 77:4494-4498.
Hynes R.O. (1973) Alteration of cell‐surface proteins by viral transformation and by proteolysis. Proc. nat. Acad. Sci. (Wash.) 70:3170-3174.
Ingber D.E., Madri J.A., Jamieson J.D. (1981) Role of basal lamina in neoplastic disorganization of tissue architecture. Proc. nat. Acad. Sci. (Wash.) 78:3901-3905.
Kefalides N.A. (1975) Basement membranes: structural and biosynthetic considerations. J. invest. Derm. 65:85-92.
Liotta L.A., Abe S., Robey P.G., Martin G.R. (1979) Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor. Proc. nat. Acad. Sci. (Wash.) 76:2268-2272.
Liotta L.A., Foidart J.M., Gehron‐Robey P., Martin G.R., Gullino P.M. (1979) Identification of micrometastasis of breast carcinomas by presence of basement membrane collagen. Lancet 2:146-147.
Orkin R.W., Gehron P., McGoodwin E.B., Martin G.R., Valentine T., Swarm R.H. (1977) A murine tumor producing a matrix of basement membrane. J. exp. Med. 145:204-220.
Recklies A.D., Tiltman K.J., Stoker A.M., Poole A.R. (1980) Secretion of proteinases from malignant and non‐malignant human breast tissue. Cancer Res. 40:550-556.
Rennard S., Berg R., Martin G.R., Foidart J.M., Gehron‐Robey P. (1980) Enzyme‐linked immunoassay for connective tissue components. Anal. Biochem. 104:205-214.
Ruoslahti E., Vaheri A., Kuusela P., Linder E. (1973) Fibroblast surface antigen. A new serum protein. Biochem. Biophys. Acta. 322:352-358.
Sainte‐Marie G. (1962) A paraffin embedding technique for studies employing immunofluorescence. Journal of Histochemistry & Cytochemistry 10:250-256.
Siegal G.P., Barsky S.H., Terranova V.P., Liotta L.A. (1981) Stages of neoplastic transformation of human breast tissue as monitored by dissolution of basement membrane components. An immunoperoxidase study. Invas. Metastasis 1:54-70.
Stenman S., Vaheri A. (1981) Fibronectin in human solid tumors. Int. J. Cancer 27:427-435.
Timpl R., Rohde H., Rovey P.G., Rennard S.I., Foidart J.M., Martin G.R. (1979) Laminin. A glycoprotein from basement membranes. J. biol. Chem. 254:9933-9937.
Vetterlein D., Bell T.E., Young P.L., Roblin R. (1980) Immunological quantitation and immunoadsorption of urokinase‐like plasminogen activators secreted by human cells. J. biol. Chem. 255:3665-3672.