[en] Serology is currently used for the diagnosis of canine sino-nasal aspergillosis (SNA). However, the accuracy of serological testing using commercially available, standardized purified antigen preparations of Aspergillus (CAPurAspAg) has only been poorly documented. The aim of the present study was to assess the diagnostic value of an agar-gel double immunodiffusion (AGDD) test and an anti-Aspergillus IgG ELISA, using CAPurAspAg and the commercially available Platelia test for the detection of serum galactomannan. Sera from 17 dogs with SNA, 18 dogs with a nasal tumour (NT), 11 dogs with lymphoplasmacytic rhinitis (LPR) and 33 control dogs were tested with the 3 methods. AGDD result was positive in 76.5% of dogs with SNA, whereas all sera from dogs with non-fungal nasal disease and control dogs were negative. A positive IgG ELISA result was obtained in 88% of dogs with SNA and in 18% of dogs with LPR. All patients with NT and control dogs had a negative IgG ELISA result. The Platelia test was positive in 24% of dogs with SNA, 11% of dogs with NT, 9% of dogs with LPR and 24% of control dogs. The results of this study suggest that (1) the detection of serum Aspergillus-specific antibodies with AGDD or ELISA, using CAPurAspAg, provides excellent specificity and good sensitivity, (2) the specificity is higher for AGDD (100%) than for ELISA (96.8%) while sensitivity is higher for ELISA (88.2%) than for AGDD (76.5%) and (3) serum galactomannan quantification with the Plateliat test is unreliable for the diagnosis of canine SNA.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Billen, Frédéric ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Peeters, Dominique ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Médecine interne des animaux de compagnie
Peters, I. R.; University of Bristol, School of clinical Veterinary Science > Division of Veterinary Pathology, Infection and Immunity
Helps, C. R.; University of Bristol, School of Clinical Veterinary Science > Division of Veterinary Pathology, Infection and Immunity
Huynen, Pascale ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
De Mol, Patrick ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
Massart, L.; Université de Liège - ULiège > Faculté Vétérinaire > Département des Productions Animales > Biostatistique, Economie, Sélection animale
Day, M. J.; University of bristol, School of Clinical Veterinary Science > Division of Veterinary Pathology, Infection and Immunity
Clercx, Cécile ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Language :
English
Title :
Comparison of the value of measurement of serum galactomannan and Aspergillus-specific antibodies in the diagnosis of canine sino-nasal aspergillosis.
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