[en] Highly porous composites scaffolds of poly-D,L-lactide (PDLLA) and poly(lactide-co-glycolide) (PLGA) containing different amounts (10, 25 and 50wt%) of bioactive glass (45S5 bioglass®) were prepared by thermally induced solid-liquid phase separation (TIPS) and subsequent solvent sublimation. The addition of increasing amounts of bioglass® into the polymer foams decreased the pore volume. Conversely, the mechanical properties of the polymer materials were improved. The composites were incubated in phosphate buffer saline at 37°C to study the in vitro degradation of the polymer by measurement of water absorption, weight loss as well as changes in the average molecular weight of the polymer and in the pH of the incubation medium as a function of the incubation time. The addition of bioglass®to polymer foams increased the water absorption and weight loss compared to neat polymer foams. However, the polymer molecular weight, determined by size exclusion chromatography, was found to decrease more rapidly and to a larger extent in absence of bioglass®. The presence of the bioactive filler was therefore found to delay the degradation rate of the polymer as compared to the neat polymer foams. Formation of hydroxyapatite on the surface of composites, as an indication of their bioactivity, was recorded by EDXA, X-ray diffractometry and confirmed by Raman spectroscopy.
Research center :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Maquet, Véronique; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Boccaccini, Aldo R.; Imperial College, London, UK > Department of Materials and Centre for Tissue Engineering and Regenerative
Pravata, Laurent; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Nothinger, I.; Imperial College, London, UK > Department of Materials and Centre for Tissue Engineering and Regenerative
Jérôme, Robert ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Language :
English
Title :
Porous poly(α-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering. I: preparation and in vitro characterisation
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Commentary :
The authors acknowledge Biomaterials (Elsevier) for allowing them to archive this paper.
Piskin E. Biodegradable polymeric matrices for bioartificial implants. Int J Artif Organs. 25:2002;434-440.
Marra KG, Choi D, Boduch K. Synthesis of new composites for bone tissue engineering: Biodegradable polymers, dendrimers and ceramics. Proceedings of the 222nd ACS National Meeting, Chicago, IL, USA, 2001.
Boccaccini A.R., Roether J.A., Hench L.L., Maquet V., Jérôme R. A composites approach to tissue engineering. Ceram Eng Sci Proc. 23:2002;805-816.
Pohunkova H., Adam M. Reactivity and the fate of some composite bioimplants based on collagen in connective tissue. Biomaterials. 16:1995;67-71.
Zhao F., Yin Y., Lu W.W., Leong C., Zhang W., Zhang J., Zhang M., Yao K. Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds. Biomaterials. 23:2002;3227-3234.
Wang M., Hench L.L., Bonfield W. Bioglass/high density polyethylene composite for soft tissue applications. preparation and evaluation J Biomed Mater Res. 42:1998;577-586.
Li S.H., Wijn J.R.D., Layrolle P., Groot K.D. Synthesis of macroporous hydroxyapatite scaffolds for bone tissue engineering. J Biomed Mater Res. 61:2002;109-120.
Li S.H., Groot K.D., Layrolle P. Bioceramic scaffold with controlled porous structure for bone tissue engineering. Key Eng Mater. 218-220(Bioceramics-14):2002;25-30.
Orefice R.L., LaTorre G.P., West J.K., Hench L.L. Processing and characterization of bioactive polysulfone-Bioglass composites. Proceedings of the International Symposium on Ceramics in Medicine. 8:1995;409-414.
Stamboulis A., Hench L.L. Bioresorbable polymers. their potential as scaffolds for bioglass composites Key Eng Mater. 192-195(Bioceramics):2001;729- 732.
Niiranen H., Pyhältö T., Rokkanen P., Paatola T., Törmälä P. Bioactive glass 13-93/P(L/DL)LA composites for in vitro and in vivo. Key Eng Mater. 192-195(Bioceramics):2001;721-724.
Dunn A.S., Campbell P.G., Marra K.G. The influence of polymer blend composition on the degradation of polymer/hydroxyapatite biomaterials. J Mater Sci Mater Med. 12:2001;673-677.
Zhang R., Ma P.X. Poly(α-hydroxyl acids)/hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology. J Biomed Mater Res. 44:1999;446-455.
Laurencin C.T., Attawia M.A., Elgendy H.E., Herbert K.M. Tissue engineered bone-regeneration using degradable polymers. the formation of mineralized matrices Bone. 19:1996;3S-9S.
Tormala P, Kellomaki M, Bonfield W. Bioactive and biodegradable composites of polymers and glasses and method to manufacture such composites. Int. Patent No. 9911296, 1999.
Sherwood J.K., Riley S.L., Palazzolo R., Brown S.C., Monkhouse D.C., Coates M., Griffith L.G., Landeen L.K., Ratcliffe A. A three-dimensional osteochondral composite scaffold for articular cartilage repair. Biomaterials. 23:2002;4739-4751.
Langer R., Vacanti J. Tissue engineering. Science. 260:1993;920-926.
Maquet V, Jérôme R. Design of macroporous biodegradable polymer scaffold for cell transplantation. In: Liu D-M, Dixit V, editors. Porous materials for tissue engineering. Uetikon-Zuerich: Trans Tech Publications Ltd.; 1997. p. 15-42.
Laurencin C.T., Attawia M.A., Elgendy H.M., Fan M. Porous polymer-ceramic systems for tissue engineering support the formation of mineralized bone matrix. Mat Res Soc Symp Proc. 414:1996;157-164.
Zhang K, Wang Y, Hillmyer MA, Francis LF. Porous polylactide/bioactive glass composites for tissue engineering applications. Proceedings of the 28th Annual Meeting of the Society for Biomaterials 2002, Tempa, Florida, USA.
Zhang R., Ma P. Porous poly(L-lactic acid)/apatite composites created by biomimetic process. J Biomed Mater Res. 45:1999;285-293.
Roether J.A., Boccaccini A.R., Hench L.L., Maquet V., Gautier S., Jérôme R. Development and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass® for tissue engineering applications. Biomaterials. 23:2002;3871-3878.
Boccaccini A.R., Notingher I., Maquet V., Jérôme R. Bioresorbable and bioactive composite materials based on polylactide foams filled with and coated by Bioglass® particles for tissue engineering applications. J Mater Sci Mater Med. 14:2003;443-450.
Maquet V., Boccaccini A.R., Pravata L., Notingher I., Jérôme R. Preparation, characterisation and in vitro degradation of bioresorbable and bioactive composites based on Bioglass®- filled polylactide foams. J Biomed Mater Res. 66A:2003;335-346.
Boccaccini A.R., Maquet V. Bioresorbable and bioactive polymer/Bioglass® composites with tailored pore structure for tissue engineering applications. Comp Sci Technol. 63:2003;2417-2429.
Maquet V., Blacher S., Pirard R., Pirard J-.P., Vyakarnam M.N., Jérôme R. Preparation of macroporous biodegradable poly(L-lactide-co-ε-caprolactone) foams and characterization by mercury intrusion porosimetry, image analysis and impedance spectroscopy. J Biomed Mater Res. 66A:2003;199-213.
Roether J.A., Gough J.E., Boccaccini A.R., Hench L.L., Maquet V., Jérôme R. Novel bioresorbable and bioactive composite based on bioactive glass and polylactide foams for bone tissue engineering. J Mater Sci Mater Med. 13:2002;1207-1214.
Roether JA. Development of novel biodegradable and bioactive composites based on biodegradable polymers and Bioglass® for tissue engineering applications. Master's thesis, Imperial College, London, 2001.
Jones J.R., Hench L.L. Biomedical materials for new millennium. perspective on the future Mater Sci Tech. 17:2001;891-900.
Hench L.L., Splinter R.J., Allen W.C., Greenlee T.K. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res. 2:1971;117-141.
Schugens C., Maquet V., Grandfils C., Jérôme R., Teyssié P. Biodegradable and macroporous polylactide implants for cell transplantation. 1. Preparation of macroporous polylactide supports by solid-liquid phase separation Polymer. 37:1996;1027-1038.
Maquet V., Blacher S., Pirard R., Pirard J-.P., Jérôme R. Characterization of porous polylactide foams by image analysis and impedance spectroscopy. Langmuir. 16:2000;10463-10470.
Ma P.X., Zhang R., Xiao G., Franceschi R. Engineering new bone tissue in vitro on highly porous poly(a-hydroxyl acids)/hydroxyapatite composite scaffolds. J Biomed Mater Res. 54:2001;284-293.
Liu Q., Wijn J.R.D., Bakker D., Blitterswijk C.A.V. Surface modification of hydroxyapatite to introduce interfacial bonding with polyactiveTM 30/70 in a biodegradable composite. J Mater Sci Mater Med. 7:1996;551-557.
Maquet V, Vyakarnam MN, Jérôme R. Macroporous scaffolds of poly(α-hydroxyacid) for tissue engineering - New morphologies obtained by freeze-drying using different solvent systems, unpublished results.
Cao W., Hench L.L. Bioactive ceramics. Ceram Int. 22:1995;493-507.
Rich J., Jaakkola T., Tirri T., Närhi T., Yli-Urpo A., Seppälä J. In vitro evaluation of poly(epsilon-caprolactone-co-DL- lactide)/bioactive glass composites. Biomaterials. 23:2002;2143-2150.
Pitt C.G., Jeffcoat A.R., Zweidinger R.A., Schindler A. Sustained drug delivery systems. I. The permeability of poly(epsilon-caprolactone), poly(D,L-lactide) and their copolymers. J Biomed Mater Res. 13:1979;497-507.
Ruffieux K, Degradables Osteosynthesesystem aus Polylactid für die maxillofaciale Chirurgie: ein Beitrag zur Werkstoff- und Prozessentwicklung 1997, ETH, Zurich.
