Poster (Scientific congresses and symposiums)
Aspergillus fumigatus detection by PCR in broncho-alveolar fluid
Hayette, Marie-Pierre; Boland, Pascal; Evrard, Béatrice et al.
1999Interscience Conference on Antimicrobial Agents and Chemotherapy (39th ICAAC).


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Keywords :
Aspergillus; bronchoalveolar fluid; PCR; diagnosis
Abstract :
[en] Background: The usefullness of a nested PCR for detection of Aspergillus fumigatus DNA was evaluated in bronchoalveolar lavage (BAL) fluid during a period of two years (1996- 1998). The aim of the study was to assess the role of PCR in diagnosing invasive pulmonary aspergillosis (IPA). Methods: A nested PCR-based amplification of fragments of genes-encoding alkaline proteases from A. fumigatus was used to test 167 BAL samples. All samples were checked for the absence of amplification inhibitors. Medical, radiological, microbiological records and autopsy findings were reviewed for assessing invasive aspergillosis. All successive patients investigated by BAL were included in the study. They were distributed in three groups: A, proven or probable aspergillosis (n=11); B, colonization (n=2); C, no evidence of IPA (n=154). PCR results were compared to culture détection as gold standard and to clinical data. Results: BAL fluids from 10 patients of group A were PCR positive. One case was falsely negative. Among group B, one case was PCR positive, and the second one PCR negative but had negative BAL cultures (only culture positive sputum). No false positive was detected among group C. Comparing to culture, sensitivity was 91%, specificity, 100%, positive predictive value, 100% and négative predictive value, 99%. Conclusion: 1. Aspergillus fumigatus PCR in BAL fluid was an accurate test to diagnose culture negative patients with IPA and to confirm culture positive samples; however it doesn't make difference between infection and colonization. 2. It is an appropriate test to exclude Aspergillus infection in patients at risk of invasive illness.
Disciplines :
Immunology & infectious disease
Laboratory medicine & medical technology
Author, co-author :
Hayette, Marie-Pierre ;  Université de Liège - ULiège > Microbiologie médicale
Boland, Pascal;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
Evrard, Béatrice;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
De Mol, Patrick ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Language :
Title :
Aspergillus fumigatus detection by PCR in broncho-alveolar fluid
Alternative titles :
[fr] Détection d'Aspergillus fumigatus dans le lavage broncho-alvéolaire
Publication date :
29 September 1999
Number of pages :
Event name :
Interscience Conference on Antimicrobial Agents and Chemotherapy (39th ICAAC).
Event organizer :
American society for microbiology
Event place :
San Francisco, United States - California
Event date :
26-29 septembre 1999
Audience :
Available on ORBi :
since 31 January 2011


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