Poster (Scientific congresses and symposiums)
Comparative evaluation of Fungitest, Neosensitabs and broth microdilution method for yeasts susceptibility testing
Hayette, Marie-Pierre; Bolland, Pascal; Seidel, Laurence et al.
1999ASM 99th general meeting


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Abstract :
[en] The need of a simple and reliable method for routine yeasts susceptibility testing led us to evaluate two commercially available methods. We investigated the in vitro susceptibility of 67 clinical isolates (26 C. albicans, 32 C. glabrata, 4 C. krusei, 2 C. tropicalis, 2 C. kefyr and 1 S. cerevisiae) to 6 drugs flucytosine (FC), amphotericin B (A), fluconazole (FZ), itraconazole (IT), kétoconazole (K), miconazole (M), comparing two methods Fungitest (Sanofi Pasteur) and Neosensitabs (Rosco). A broth microdilution adaptation from the NCCLS-M27A procedure was used as reference method. Fungitests consist of individually packed 16 wells microplates containing 6 drugs at two critical concentrations in buffered medium. Reading was performed after 24 and 48h incubation. Neosensitabs is an agar diffusion method on Shadomy agar using antifungals tablets. Reading was performed after 24h. For all strains Neosensitabs was in concordance with NCCLS M27-A for FC (94%), A (98%), FZ (55%) I (53%) with p<0.05. Fungitest correlated with NCCLS method for all antifungals after 24 and 48h incubation time (p<0.05) with respectively 95/95% for FC, 100/100% for A, 80/76% for FZ, 81/55% for I and 93/75% for K, and 88/81 for M. Candida glabrata gave the poorest result with Neosensitabs with 28% concordance for FZand 39% for I; therefore the method can't be recommended for this species. Fungitest concordance observed was 55% for I after 48h. Our results suggest that Fungitest is appropriate for routine yeast susceptibility testing. However itraconazole testing has to be improved with this method.
Disciplines :
Laboratory medicine & medical technology
Immunology & infectious disease
Author, co-author :
Hayette, Marie-Pierre ;  Université de Liège - ULiège > Microbiologie médicale
Bolland, Pascal
Seidel, Laurence  ;  Université de Liège - ULiège > Département des sciences de la santé publique > Informatique médicale et biostatistique
Welter, S.
Melin, Pierrette  ;  Université de Liège - ULiège > Microbiologie médicale
De Mol, Patrick ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Language :
Title :
Comparative evaluation of Fungitest, Neosensitabs and broth microdilution method for yeasts susceptibility testing
Publication date :
May 1999
Event name :
ASM 99th general meeting
Event organizer :
American society for microbiology
Event place :
Chicago, United States - Illinois
Event date :
30 May-3 June 1999
Audience :
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since 28 January 2011


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