Reference : Comparison of the Sensititre YeastOne and Fungitest methods with the NCCLS M27-A2 re...
Scientific congresses and symposiums : Poster
Human health sciences : Immunology & infectious disease
Human health sciences : Laboratory medicine & medical technology
Comparison of the Sensititre YeastOne and Fungitest methods with the NCCLS M27-A2 reference method for antifungal susceptibility testing of yeasts
Hayette, Marie-Pierre mailto [Université de Liège - ULiège > > Microbiologie médicale >]
Kondarowski, E. [ > > ]
Melin, Pierrette mailto [Université de Liège - ULiège > > Microbiologie médicale >]
Tsobo, Chantal [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale > >]
Huynen, Pascale mailto [Université de Liège - ULiège > > Microbiologie médicale >]
De Mol, Patrick mailto [Université de Liège - ULiège > > Microbiologie médicale >]
Interscience Conference on Antimicrobial Agents and Chemotherapy (45th : 2005 : Washington).
16-19 december 2005
American society for microbiology
[en] Sensititre ; fungitest ; in vitro susceptibility testing ; Candida ; NCCLS
[en] Background: The recent introduction of Sensititre YeastOne, a colorimetric microdilution method that includes new antifungal agents has opened the field to MIC’s determination by an easy-to-perform method. The aim of this study was to compare this test with the NCCLS M27-A protocol and with Fungitest, a current routine method for yeasts susceptibility testing.
Methods: Sensititre YeastOne method (Trek diagnostic), and the NCCLS M27-A2 were performed on 300 yeasts clinical isolates distributed as follow: 125 C. albicans, 273 non-albicans species. Four antifungal agents were tested by the reference method: amphotericine B (AmB), fluconazole (FZ), itraconazole (ITZ), and voriconazole (VOR). The reading of the Sensititre and NCCLS results was visually performed after 24 and 48 h respectively. The Fungitest (Biorad) method was applied on 121 among the 300 isolates and the reading was made after 24 to 48 h incubation time according to the positive control growth.
Results: By the NCCLS method, the MICs50/MICs90 (µg/ml) were as follows: 1/2 (AmB); 16/64 (FZ); 0.25/4 (ITZ) and 0.125/2 (VOR). Sensititre vs. NCCLS: The overall agreement within 2 dilutions for AmB, FZ, ITZ and VOR was respectively 54, 82, 80 and 78%. The MICs50/MICs90 were in absolute concordance for VOR by both techniques. Very major errors (%) were recorded as follows: 0.01/0 (AmB with a MIC ≥ 4/8µg/ml for resistant strains respectively), 1.6 (FZ), 3.6 (ITZ) and 2.3 (VOR with a MIC ≥ 8µg/ml for resistant strains). Fungitest vs. NCCLS: The agreement between both methods including minor discrepancies was 98% (AmB), 88% (FZ) and 98% (ITZ). Following the breakpoints given by the manufacturer, very major errors were 6.3% for FZ, 0.03% for ITZ and none for AmB.
Conclusions: Sensititre is a convenient alternative to the NCCLS method for yeast susceptibility testing. Fungitest in spite of good correlations must change the breakpoints and include new antifungal agents to be competitive.

File(s) associated to this reference

Fulltext file(s):

Open access
SENSITITRE.pdfAuthor postprint905.14 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.