Reference : In Vitro Susceptibility Testing of Aspergillus fumigatus against Posaconazole: Compar...
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Human health sciences : Immunology & infectious disease
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In Vitro Susceptibility Testing of Aspergillus fumigatus against Posaconazole: Comparison of NCCLS M38-P and E-Test Methods
Hayette, Marie-Pierre mailto [Université de Liège - ULiège > > Microbiologie médicale >]
Amadore, Agatha [ > > ]
Seidel, Laurence mailto [Université de Liège - ULiège > Département des sciences de la santé publique > Informatique médicale et biostatistique >]
Christiaens, Geneviève mailto [Université de Liège - ULiège > > Direction médicale >]
Garrino, M. G. [Université Catholique de Louvain - UCL Montgodinne > Microbioloige médicale > > >]
Melin, Pierrette mailto [Université de Liège - ULiège > > Microbiologie médicale >]
Glupzcynski, Youri [Université Catholique de Louvain - UCL Montgodinne > Microbiologie médicale > > >]
De Mol, Patrick mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale >]
Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
27-30 september 2002
American society for microbiology
San Diego, California
[en] posaconazole ; in vitro suceptibility ; E-test ; Aspergillus fumigatus
[en] Posaconazole is a second-generation triazole and structural analogue of itraconazole. This drug has fungicidal activity against yeasts and filamentous fungi. The aim of our study was to evaluate E-test method for in vitro susceptibility testing of Aspergillus fumigatus isolates against posaconazole. METHODS: A total of 121 isolates of A. fumigatus were selected as follows: 106 clinical strains from colonized patients, 18 from patients with invasive aspergillosis and 7 environmental isolates. Their in vitro susceptibility was evaluated by E-test (Abbiodisk, Sweden) and compared with NCCLS microdilution reference method (M38-P). Both tests were performed with RPMI 1640 medium at 35 degrees C. MIC values were read after 24h (MIC-24h) and 48h (MIC-48h) incubation time by E-test method. Two MIC endpoints were determined by NCCLS method: visible reduction of growth (MIC-0); 2. 50% reduction (or more) of growth (MIC-2). Three A. fumigatus reference strains (IHEM 5734, 6149 and 13935) were included as control. RESULTS: Geometric mean MICs (microg/ml) were respectively 0.02 for E-test at 24h and 0.029 at 48h. MIC-0 and MIC-2 values were respectively 0.19 and 0.018 microg/ml. One correlation between both methods was observed for MICs-24h and MICs-2s (p<.05). However, there was no significant difference according to origin of isolates (p<.05). CONCLUSIONS: 1. This study assessed the potent role of posaconazole against A. fumigatus isolates with very low MICs. 2. MIC values were not predictive of pathogenicity. 3. E-test method by reading after 24h-incubation time could easily replace the time-consuming NCCLS M38-P reference method.
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