Article (Scientific journals)
How to monitor NF-kappaB activation after photodynamic therapy.
Coupienne, Isabelle; Piette, Jacques; Bontems, Sébastien
2010In Methods in Molecular Biology, 635, p. 79-95
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Keywords :
Active Transport, Cell Nucleus/drug effects/radiation effects; Aminolevulinic Acid/pharmacology/therapeutic use; Animals; Base Sequence; Blotting, Western; Cell Nucleus/drug effects/metabolism/radiation effects; Cytoplasm/drug effects/metabolism/radiation effects; Gene Expression Regulation, Neoplastic/drug effects/radiation effects; Genes, Reporter/genetics; Hela Cells; Humans; I-kappa B Proteins/metabolism; Luciferases/genetics; NF-kappa B/isolation & purification/metabolism; Photochemotherapy; Photosensitizing Agents/pharmacology/therapeutic use; Synaptotagmin I/metabolism
Abstract :
[en] The nuclear factor-kappa B (NF-kappaB) is a multipotent factor involved in many cellular processes such as inflammation, immune response and embryonic development and it can be activated by a large number of stimuli. Consequently, this transcription factor plays a pivotal role in many natural processes but also in different pathologies. For several years, photodynamic therapy (PDT) has emerged as an attractive alternative approach for the treatment of different affections involving various forms of cancer and an increasing number of reports have highlighted the activation of the NF-kappaB following PDT treatment. Furthermore, it has been shown that the mechanism of activation of the NF-kappaB as well as its target genes depends on the nature of the photosensitizers and the cell type used. As this transcription factor is known to be a key regulator of the immune response but also controls cell survival and proliferation, it is important to assess its activation status and its impact on the target genes. In this review, we will present different techniques allowing identification of the activation status of this factor, from the degradation of its inhibitor in the cytoplasm to its ability to induce the expression of a reporter gene under the control of a target promoter. As a working model we will present results obtained from a 5-aminolevulinic acid-PDT treatment on cervix adenocarcinoma cells.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Coupienne, Isabelle ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research
Bontems, Sébastien ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Language :
English
Title :
How to monitor NF-kappaB activation after photodynamic therapy.
Publication date :
2010
Journal title :
Methods in Molecular Biology
ISSN :
1064-3745
eISSN :
1940-6029
Publisher :
Humana Press, Totowa, United States - New Jersey
Volume :
635
Pages :
79-95
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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