Constitutive nuclear factor-kappa B activity preserves homeostasis of quiescent mature lymphocytes and granulocytes by controlling the expression of distinct Bcl-2 family proteins

Bureau, Fabrice; Vanderplasschen, Alain; Jaspar, Fabrice; Minner, F.; Pastoret, Paul-Pierre; Merville, M. P.; Bours, Vincent; Lekeux, Pierre

doi:10.1182/blood.V99.10.3683

Article (Scientific journals)

Constitutive nuclear factor-kappa B activity preserves homeostasis of quiescent mature lymphocytes and granulocytes by controlling the expression of distinct Bcl-2 family proteins

Bureau, Fabrice; Vanderplasschen, Alain; Jaspar, Fabrice et al.

2002 • In Blood, 99 (10), p. 3683-3691

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https://hdl.handle.net/2268/8121

DOI
10.1182/blood.V99.10.3683

PubMed
11986224

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Abstract :

[en] Constitutive nuclear factor kappaB (NFkappaB) activity protects quiescent mature Immune cells from spontaneous apoptosis. Here, we examined whether NF-kappaB exerts its antiapoptotic function in these cells through the control of Bcl-2 family proteins. Specific pharmacologic inhibitors of NF-kappaB were used to achieve total NF-kappaB inactivation In quiescent human blood lymphocytes, granulocytes, and monocytes. NF-kappaB inhibition induced drastic lymphocyte and granulocyte apoptosis, but only moderate monocyte apoptosis. T- and B-cell apoptosis was slow and associated with a gradual down-regulation of the prosurvival Bcl-2 family proteins Bcl-X-L and BcI-2, respectively. By contrast, granulocyte apoptosis was fast and accompanied by a rapid cellular accumulation of Bcl-x(s), the proapoptotic Bcl-x isoform that is generated from alternative splicing of the bcl-x pre-mRNA. Finally, antisense bci-x(L) and bcl-2 knockdown in T and B cells, respectively, and induction of Bcl-xs expression in granulocytes through antisense oligonucleotide-mediated redirection of bcl-x pre-mRNA splicing were sufficient to induce significant apoptosis in these cells. Taken together, these results reveal that basal NF-kappaB activity preserves homeostasis of quiescent mature lymphocytes and granulocytes through regulation of distinct members of the Bcl-2 family. This study sheds light on the constitutive mechanisms by which NF-kappaB maintains defense integrity. (C) 2002 by The American Society of Hematology.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology
Veterinary medicine & animal health

Author, co-author :

Bureau, Fabrice ; Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie et biologie moléculaire

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

Jaspar, Fabrice ; Université de Liège - ULiège > Clinique des grands animaux

Minner, F.

Pastoret, Paul-Pierre ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires

Merville, M. P.

Bours, Vincent

Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie

Language :

English

Title :

Constitutive nuclear factor-kappa B activity preserves homeostasis of quiescent mature lymphocytes and granulocytes by controlling the expression of distinct Bcl-2 family proteins

Publication date :

May 2002

Journal title :

Blood

ISSN :

0006-4971

eISSN :

1528-0020

Publisher :

American Society of Hematology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

3683-3691

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 09 March 2009

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