Deletion of the 5'-ABL region: a recurrent anomaly detected by fluorescence in situ hybridization in about 10% of Philadelphia-positive chronic myeloid leukaemia patients.
Adult; Aged; Aged, 80 and over; DNA Probes; Female; Fusion Proteins, bcr-abl/genetics; Gene Deletion; Humans; In Situ Hybridization, Fluorescence; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics; Male; Middle Aged; Philadelphia Chromosome
Abstract :
[en] Inclusion of the BCR-ABL ES probe in routine cytogenetics led to the identification of a subgroup of Philadelphia positive (Ph+) chronic myeloid leukaemia patients characterized by a 5'-ABL deletion. This anomaly was observed in 5/51 cases (9.8%). Cytological and clinical data suggest that the 5'-ABL deletion may be associated with dysplastic features of polymorphonuclear cells and metamyelocytes and a short chronic phase duration.
Disciplines :
Hematology
Author, co-author :
Herens, Christian ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
Tassin, Françoise ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Lemaire, Véronique ; Centre Hospitalier Universitaire de Liège - CHU > Salles urgences NDB
Beguin, Yves ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
Collard, E.
Lampertz, S.
Croisiau, C.
Desouter-Lecomte, Michèle ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de chimie physique théorique
Koulischer, Lucien ; Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Deletion of the 5'-ABL region: a recurrent anomaly detected by fluorescence in situ hybridization in about 10% of Philadelphia-positive chronic myeloid leukaemia patients.
Bentz M., Cabot G., Moos M., Speicher M.R., Ganser A., Lichter P., Dohner H. (1994) Detection of chimeric BCR-ABL genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization. Blood 83:1922-1928.
Buno I., Wyatt W.A., Zinsmeister A.R., Dietz-Band J., Silver R.T., Dewald G.W. (1998) A special fluorescent in situ hybridization technique to study peripheral blood and assess the effectiveness of interferon therapy in chronic myeloid leukemia. Blood 92:2315-2321.
Dewald G.W., Wyatt W.A., Juneau A.L., Carlson R.O., Zinsmeister A.R., Jalal S.M., Spurbeck J.L., Silver R.T. (1998) Highly sensitive fluorescent in situ hybridization method to detect double BCR/ABL fusion and monitor response to therapy in chronic myeloid leukemia. Blood 91:3357-3365.
Dewald G.W., Wyatt W.A., Silver R.T. (1999) Atypical BCR and ABL D-FISH patterns in chronic myeloid leukemia and their possible role in therapy. Leukemia and Lymphoma 34:481-491.
Melo J.V. (1996) The molecular biology of chronic myeloid leukemia. Leukemia 10:751-756.
Rowley J.D. (1973) A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and giemsa staining. Nature 243:290-193.
Sawyers C.L. (1999) Chronic myeloid leukemia. New England Journal of Medicine 340:1330-1340.
Sinclair P.B., Green A.R., Grace C., Nacheva E.P. (1997) Improved sensitivity of BCR-ABL detection: A triple-probe three-color fluorescence in situ hybridization system. Blood 90:1395-1402.