Diagnosis and Screening for Cytomegalovirus Infection in Pregnant Women in Cuba as Pronostic Markers of Congenital Infection in Newborns: 2007-2008

Kouri, Vivian; Correa, Consuelo B; Verdasquera, Denis; Martinez, Pedro Ariel; Alvarez, Alina; Aleman, Yoan; Perez, Lissette; Golpe, Maria A; Someilan, Tatiana; Chong, Yodila; Fresno, Clara; Navarro, Maria A; Perez, Elsa; Moro, Ivonne; Sanchez, Rita; Llanusa, Celia; Melin, Pierrette

doi:10.1097/INF.0b013e3181eb7388

Article (Scientific journals)

Diagnosis and Screening for Cytomegalovirus Infection in Pregnant Women in Cuba as Pronostic Markers of Congenital Infection in Newborns: 2007-2008

Kouri, Vivian; Correa, Consuelo B; Verdasquera, Denis et al.

2010 • In The Pediatrics Infectious Disease Journal, 29 (12), p. 1105-1110

Peer reviewed

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https://hdl.handle.net/2268/80245

DOI
10.1097/INF.0b013e3181eb7388

PubMed
20622711

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Keywords :

congenital Infection; HCMV; Cuba; Seroprevalence; Viral excretion; PCR; pregnant women

Abstract :

[en] Background: Human cytomegalovirus (HCMV) has established itself as the most significant cause of congenital infection in the developed world. The objective of this research was prenatal identification of pregnant women at risk for developing active infection due to HCMV as well as to diagnose congenitally infected newborns. Methods: A diagnostic algorithm based on specific immunoglobulin G (IgG), IgM, and, IgG avidity was used to screen serum from 1131 pregnant women enrolled prospectively from 3 municipalities from Havana City, Cuba during 2007–2008. Qualitative multiplex nested PCR and quantitative real time-based PCR testing for HCMV DNA were performed on urine and saliva specimens from women detected with active infection and from their newborns. Results: Most women were seropositive to HCMV (92.7%), with 2.38% (27 women) having active infection. Primary infection was detected in 20 pregnant women (1.77%) while 7 patients (0.62%) had active nonprimary infection. HCMV DNA was detected in specimens from 9 of the 27 pregnant women by both PCR methods. HCMV congenital infection was diagnosed in 12 (1.06%) of the 26 live children born from 25 mothers with active infection, for a vertical transmission rate of 46.2%. Two fetal deaths were reported from 2 women with active infection; furthermore 2 newborns were symptomatic at birth and 2 showed sequelae during the follow-up done until 6 months age. Conclusions: Mothers with active infection during the pregnancy and with HCMV excretion had significant risks, RR = 1.16 and RR = 1.35, respectively, to have congenitally infected children.

Research center :

Institut of Tropical Medicine Pedro Kouri, Havana, Cuba
Microbiologie Médicale, CHU de Liège, Université de Liège, Belgique

Disciplines :

Reproductive medicine (gynecology, andrology, obstetrics)
Immunology & infectious disease
Laboratory medicine & medical technology

Author, co-author :

Kouri, Vivian; Institute of Tropical Medicine Pedro Kouri, Havana City, Cuba > Virology Department

Correa, Consuelo B; Institute of Tropical Medicine Pedro Kouri, Havana City, Cuba > Department of Virology

Verdasquera, Denis; Institute of Tropical Medicine Pedro Kouri, Havana City, Cuba > Department of Virology

Martinez, Pedro Ariel; Institute of Tropical Medicine Pedro Kouri, Havana City, Cuba > Department of Virology

Alvarez, Alina; Institute of Tropical Medicine Pedro Kouri, Havana City, Cuba > Department of Virology

Aleman, Yoan; Institute of Tropical Medicine Pedro Kouri, Havana City, Cuba > Department of Virology

Perez, Lissette; Institute of Tropical Medicine Pedro Kouri, Havana City, Cuba > Department of Virology

Golpe, Maria A; Genetics Department of Municipalities: Regla, Old Havana, Central Havana ; Cuba

Someilan, Tatiana; Genetics Department of Municipalities: Regla, Old Havana, Central Havana ; Cuba

Chong, Yodila; Genetics Department of Municipalities: Regla, Old Havana, Central Havana ; Cuba

More authors (7 more)

Language :

English

Title :

Diagnosis and Screening for Cytomegalovirus Infection in Pregnant Women in Cuba as Pronostic Markers of Congenital Infection in Newborns: 2007-2008

Publication date :

December 2010

Journal title :

The Pediatrics Infectious Disease Journal

ISSN :

0891-3668

Publisher :

Lippincott Williams & Wilkins

Volume :

Issue :

Pages :

1105-1110

Peer reviewed :

Peer reviewed

Name of the research project :

Epidémiologie cubaine de l’infection congénitale par le cytomegalovirus et diagnostic prénatal

Funders :

DGRE - Région wallonne. Direction générale des Relations extérieures [BE]

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