A direct enzyme-linked immunosorbent assay (Elisa) for antibodies to enterobacterial score glycolipid and lipid A. Results in Healthy Subjects and in Patients Infected by Gram-Negative Bacteria
[en] We have developed an ELISA for IgM and IgG antibodies to the core glycolipid (CGL) of the Re mutant Salmonella minnesota R 595, and to lipid A. Anti-CGL antibodies have been detected in sera from 37% of healthy blood donors, whereas anti-lipid A activities were found in 13% of individuals only. The anti-CGL and anti-lipid A activities were examined in patients in a surgical intensive care unit, selected on the basis of a definite risk of infectious complications due to Gram-negative bacteria. Of the patients who developed such infections, the rate of favourable outcome was significantly higher in patients with either stable positive or increasing anti-CGL activities than in patients found to be negative. Our results provide clear evidence that anti-CGL antibodies contribute to host defence against various Gram-negative bacteria.
Disciplines :
Anesthesia & intensive care
Author, co-author :
Nys, Monique ; Centre Hospitalier Universitaire de Liège - CHU > Soins intensifs
Damas, Pierre ; Université de Liège - ULiège > Soins intensifs
Damas, François ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Joassin, L.
Demonty, Jean ; Centre Hospitalier Universitaire de Liège - CHU > Maladies infectieuses et médecine interne générale
Language :
English
Title :
A direct enzyme-linked immunosorbent assay (Elisa) for antibodies to enterobacterial score glycolipid and lipid A. Results in Healthy Subjects and in Patients Infected by Gram-Negative Bacteria
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