cryptolepine; triplex; quadruplex; mass spectrometry; telomerase; DNA; noncovalent
Abstract :
[en] Cryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a preference for GC-rich sequences. In this study, dialysis competition assay and mass spectrometry experiments were used to determine the affinity of cryptolepine and neocryptolepine for DNA structures among duplexes, triplexes, quadruplexes and single strands. Our data confirm that cryptolepine and neocryptolepine prefer GC over AT-rich duplex sequences, but also recognize triplex and quadruplex structures. These compounds are weak telomerase inhibitors and exhibit a significant preference for triplexes over quadruplexes or duplexes. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Societe francaise de biochimie et biologic moleculaire. All rights reserved.
Research Center/Unit :
Giga-Systems Biology and Chemical Biology - ULiège CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Guittat, Lionel; Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM UR 565, CNRS UMR 8646, 43, rue Cuvier, 75231 Paris cedex 5, France
Alberti, Patrizia; Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM UR 565, CNRS UMR 8646, 43, rue Cuvier, 75231 Paris cedex 5, France
Rosu, Frédéric ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Van Miert, Sabine; Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium
Thetiot, Emilie; Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM UR 565, CNRS UMR 8646, 43, rue Cuvier, 75231 Paris cedex 5, France
Pieters, Luc; Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium
Gabelica, Valérie ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Ottaviani, Alexandre; Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM UR 565, CNRS UMR 8646, 43, rue Cuvier, 75231 Paris cedex 5, France
Riou, Jean-François; Laboratoire de Physiologie Humaine, UFR de Pharmacie, IFR 53, Université de Reims Champagne-Ardenne, 51, rue Cognacq-Jay, 51096 Reims cedex, France
Mergny, Jean-Louis; Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM UR 565, CNRS UMR 8646, 43, rue Cuvier, 75231 Paris cedex 5, France
Language :
English
Title :
Interactions of cryptolepine and neocryptolepine with unusual DNA structures
Publication date :
2003
Journal title :
Biochimie
ISSN :
0300-9084
eISSN :
1638-6183
Publisher :
Elsevier, Paris, France
Volume :
85
Issue :
5
Pages :
535-547
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Commentary :
This is a postprint author version of an article published in: Biochimie (2003), 85, 535-547.
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