Reference : Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies
Bargnoux, A. S. [> >]
Cavalier, Etienne mailto [Université de Liège - ULiège > > Chimie médicale >]
Cristol, J. P. [> >]
Simon, N. [> >]
Dupuy, A. M. [> >]
Garrigue, V. [> >]
Delanaye, Pierre mailto [Université de Liège - ULiège > > Néphrologie >]
Mourad, G. [> >]
Clinical Chemistry & Laboratory Medicine
Walter de Gruyter
Yes (verified by ORBi)
[en] Cystatin C ; renal transplantation ; glomerular filtration rate ; predictive equation
[en] Background: The potential use of Cystatin C was recently assessed in kidney transplantation.
A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies
(Binding Site Human Cystatin C immunoassay) has been developed. Analytical performance
of this new assay was evaluated. Clinical relevance was determined by comparison with a
reference method in a cohort of kidney transplant patients.
Patients and methods: First, the analytical performance of the Binding Site cystatin C kit
was tested on SPAPLUS® and Hitachi® analyzers. Second, a comparison study was performed
using SPAPLUS® analyzer against two other cystatin C methods (the Siemens-PENIA method
on BNII® and the Dako-PETIA application on Olympus AU640®). Third, the glomerular
filtration rate (GFR) was estimated using several predictive cystatin C- and creatinine-based
equations and compared to GFR measured by an isotopic method (99mTc-DTPA). These
predictive algorithms were analyzed with respect to bias, precision and accuracy.
Results: Total intra-assay and inter-assay coefficients of variation were below 5%. Values
obtained with the SPAPLUS® correlated with the Siemens-PENIA and the Dako-PETIA
methods. The creatinine and cystatin C-based equation allowed reliable assessment of GFR in
our population of renal transplantation.
Conclusions: The use of algorithms based on cystatin C and creatinine could provide a
reliable estimate of GFR in kidney transplantation.

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