Comparison of Biotyping, Ribotyping, and Pulsed-Field Gel Electrophoresis for Investigation of a Common-Source Outbreak of Burkholderia Pickettii Bacteremia
[en] Over a 3-month period, six immunocompromised patients developed one or more episodes of Burkholderia pickettii bacteremia and/or catheter infection. Vials of a commercially available, "sterile" saline for injection which had been used for flushing the patients' indwelling intravenous devices were implicated as the common source of the organisms. No further cases were diagnosed once the use of this saline was discontinued. Twenty-six isolates, including 9 outbreak-related strains from case patients and contaminated saline as well as 17 control strains, were tested comparatively by biotyping, ribotyping with EcoRI and HindIII, and pulsed-field gel electrophoresis (PFGE) with SpeI. Macrorestriction analysis revealed nine PFGE groups and was more discriminating than ribotyping (seven ribotypes) and biotyping (two biovars). Among the outbreak-related isolates, one B. pickettii type was found by the three typing methods. Furthermore, PFGE was useful for subdividing ribotypes and for distinguishing isolates involved in the outbreak from all epidemiologically unrelated strains.
Disciplines :
Laboratory medicine & medical technology Microbiology
Author, co-author :
Chetoui, H.; Université de Liège - ULiège > Microbiologie médicale
Melin, Pierrette ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Struelens, M. J.; Cliniques Universitaires de Bruxelles - Hôpital Erasme > Microbiologie > épidémiologie moléculaire
Delhalle, E.; Université de Liège - ULiège > Microbiologie Médicale
Nigo, M. M.; Université de Liège - ULiège > Microbiologie médicale
De Ryck, R.; Cliniques Universitaires de Bruxelles - Hôpital Erasme > microbiologie > épidémiologie moléculaire
De Mol, Patrick ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Language :
English
Title :
Comparison of Biotyping, Ribotyping, and Pulsed-Field Gel Electrophoresis for Investigation of a Common-Source Outbreak of Burkholderia Pickettii Bacteremia
Publication date :
June 1997
Journal title :
Journal of Clinical Microbiology
ISSN :
0095-1137
eISSN :
1098-660X
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
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