[en] This paper investigates the biohydrogen production by two mesophilic strains, a strict anaerobe (Clostridium butyricum CWBI1009) and a facultative anaerobe (Citrobacter freundii CWBI952). They were cultured in pure and mixed cultures in serum bottles with five different carbon sources. The hydrogen yields of pure C. freundii cultures ranged from 0.09 molH2.molhexose-1 (with sucrose) to 0.24 molH2.molhexose-1 (with glucose). Higher yields were obtained by the pure cultures of Cl. butyricum ranging from 0.44 molH2.molhexose-1 (with sucrose) to 0.69 molH2.molhexose-1 (with lactose). This strain also fermented starch whereas C. freundii did not. However, it consumed the other substrates faster and produced hydrogen earlier than Cl. butyricum. This ability has been used to promote the growth conditions of Cl. butyricum in co-culture with C. freundii, since Cl. butyricum is extremely sensitive to the presence of oxygen which strongly inhibits H2 production. This approach could avoid the addition of any expensive reducing agents in the culture media such as L-cysteine since C. freundii consumes the residual oxygen. Thereafter, co-cultures with glucose and starch were investigated: hydrogen yields decreased from 0.53 molH2.molhexose-1 for pure Cl. butyricum cultures to 0.38 molH2.molhexose
-1 for mixed culture with glucose but slightly increased with starch (respectively 0.69 and 0.73 molH2.molhexose-1). After 48 h of fermentation, metabolites analysis confirmed with microbial observation, revealed that the cell concentration of C. freundii dramatically decreased or was strongly inhibited by the development of Cl. butyricum.
Research center :
Centre Wallon de Biologie Industrielle
Disciplines :
Microbiology Biotechnology
Author, co-author :
Beckers, Laurent ✱; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Hiligsmann, Serge ✱; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Hamilton, Christopher ; Université de Liège - ULiège > Centre Wallon de biologie industrielle
Masset, Julien ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles - Bio-industries
✱ These authors have contributed equally to this work.
Language :
English
Title :
Fermentative hydrogen production by Clostridium butyricum CWBI1009 and Citrobacter freundii CWBI952 in pure and mixed cultures
Publication date :
2010
Journal title :
Biotechnologie, Agronomie, Société et Environnement
ISSN :
1370-6233
eISSN :
1780-4507
Publisher :
Presses Agronomiques de Gembloux, Gembloux, Belgium
Special issue title :
2e journée de réflexion de l'EDT GEPROC : Génie des procédés appliqué aux bio-industries. 16 décembre 2009, Faculté universitaire des Sciences agronomiques, Gembloux, Belgique
Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation)
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] Action de Recherches concertées ARC 07/12 04- ULg- Communauté française FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE] DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
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