Crystallization of a genetically engineered water-soluble primary penicillin target enzyme. The high molecular mass PBP2x of Streptococcus pneumoniae.

Charlier, Paulette; Buisson, G.; Dideberg, O.; Wierenga, J.; Keck, W.; Laible, G.; Hakenbeck, R.

doi:10.1006/jmbi.1993.1452

Article (Scientific journals)

Crystallization of a genetically engineered water-soluble primary penicillin target enzyme. The high molecular mass PBP2x of Streptococcus pneumoniae.

Charlier, Paulette; Buisson, G.; Dideberg, O. et al.

1993 • In Journal of Molecular Biology, 232 (3), p. 1007-9

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https://hdl.handle.net/2268/77981

DOI
10.1006/jmbi.1993.1452

PubMed
8355266

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Keywords :

Bacterial Proteins; Carrier Proteins/chemistry; Crystallization; Hexosyltransferases; Muramoylpentapeptide Carboxypeptidase/chemistry; Penicillin-Binding Proteins; Peptidyl Transferases; Protein Conformation; Protein Engineering; Recombinant Proteins/chemistry; Solubility; Streptococcus pneumoniae/enzymology; Water; X-Ray Diffraction

Abstract :

[en] A genetically engineered water-soluble derivative of PBP2x of Streptococcus pneumoniae has been produced, purified and crystallized in a form suitable for X-ray diffraction analysis. The best crystals have been grown at 15 degrees C, from solutions containing 8% polyethylene glycol 10,000 at pH values ranging from 3.9 to 6.0. These crystals diffract to a resolution of 3.5 A and have a space group P6(1)22 (or enantiomorph) with unit cell dimensions of a = b = 162.2 A, c = 171.8 A, alpha = beta = 90 degrees, gamma = 120 degrees. The molecular mass and cell dimensions suggest that there is one molecule of enzyme per asymmetric unit. The breakdown of a chromogenic cephalosporin derivative diffused into a crystal reveals clearly that the enzyme is active in the crystalline state.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Charlier, Paulette ; Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques

Buisson, G.

Dideberg, O.

Wierenga, J.

Keck, W.

Laible, G.

Hakenbeck, R.

Language :

English

Title :

Crystallization of a genetically engineered water-soluble primary penicillin target enzyme. The high molecular mass PBP2x of Streptococcus pneumoniae.

Publication date :

1993

Journal title :

Journal of Molecular Biology

ISSN :

0022-2836

eISSN :

1089-8638

Publisher :

Academic Press, London, United Kingdom

Volume :

232

Issue :

Pages :

1007-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 November 2010

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