Inheritance of sex in two ZZ pseudofemale lines of tilapia Oreochromis aureus

[en] This paper reports a study on the sex determination system of the blue tilapia, Oreochromis aureus. Investigations were carried out using a pseudofemale line in two populations of O. aureus, known as Egyptian Population (EP) and Israel Population (IP). In O. aureus, males are the homogametic sex (ZZ/ZW), and sex reversal of fry with estradiol results in the production of some functional sex-reversed fish with a female phenotype and ZZ male genotype, known as pseudofemales or A-females. Crosses between ZZ pseudofemales and ZZ males theoretically should provide monosex ZZ male progeny only. We have studied the sex ratios of progeny from 43 IP (F-2 to F-3 generations) and 51 EP (F-1 to F-5 generations), pair-matings between normal males and pseudofemales. In IP, the male percentage in progenies ranged between 83% to 100% in F-2 and 66% to 100% in F-3. In EP, male percentage was more constant, varying from 88% to 100% in F-1, from 96% to 100% in F-3 and from 97% to 100% in F-5. In EP, F-2 and F-4 pseudofemales produced only monosex male progeny. This apparent difference in sex ratio frequency distributions between the two O. aureus pseudofemale lines could be due to the selection of males. EP pseudofemales were mated with their siblings for F-2 and F-3 pseudofemales or with closely related males for F-4 and F-5 pseudofemales. Conversely, IP pseudofemales were crossed with nonrelated males originating from research center broodstock, resulting in a higher proportion of females in sex ratio of progenies from successive generations of pseudofemales. The role of inbreeding is discussed in the context of predominantly monofactorial sex chromosome determination system operating in this species, influenced by other factors (genetic and environmental). The present study also shows that it is possible to fix the male sex determining factors (Z sex chromosome and genetic factors) in a line of pseudofemales, producing a high percentage of male progeny in five successive generations. (C) 2003 Elsevier Science B.V. All rights reserved.

Disciplines :

Aquatic sciences & oceanology

Author, co-author :

Desprez, D.

Mélard, Charles ; Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)

Hoareau, M. C.

Bellemene, Y.

Bosc, P.

Baroiller, J. F.

Language :

English

Title :

Inheritance of sex in two ZZ pseudofemale lines of tilapia Oreochromis aureus

Publication date :

27 March 2003

Journal title :

Aquaculture

ISSN :

0044-8486

eISSN :

1873-5622

Publisher :

Elsevier Science Bv, Amsterdam, Netherlands

Volume :

218

Issue :

1-4

Pages :

131-140

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 November 2010

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Bibliography

Abucay, J.S., Mair, G.C., Skibinski, D.O.F., Beardmore, J.A., 1999. Environmental sex determination: the effect of temperature and salinity on sex ratio in Oreochromis niloticus. Aquaculture 173, 219-234.
Avtalion, R.R., Don, J., 1990. Sex-determining genes in tilapia: a model of genetic recombination emerging from sex-ratio results of three generations of diploid gynogenetic Oreochromis aureus. J. Fish Biol. 37, 167-173.
Baroiller, J.F., 1996. Significant proportions of unexpected males in progenies from single pair matings with sibling sex-reversed males of Oreochromis niloticus. In: Pullin, R.S.V., Lazard, J., Legendre, M., Amon Kothias, J.B., Pauly, D. (Eds.), Proceedings of the Third Symposium on Tilapia in Aquaculture. ICLARM Conf. Proc., vol. 41, pp. 229-237.
Baroiller, J.F., Clota, F., 1997. Interactions between temperature effects and genotype on Oreochromis niloticus sex determination. J. Exp. Zool. 281, 507 (Abstract).
Baroiller, J.F., Chourrout, D., Fostier, A., Jalabert, B., 1995. Temperature and sex chromosomes govern sex-ratios of the mouthbrooding cichlid fish Oreochromis niloticus. J. Exp. Zool. 273, 216-223.
Chourrout, D., 1988. Revue sur le déterminisme génétique du sexe des poissons téléostéens. Bull. Soc. Zool. Fr. 113, 123-144 (in French with English abstract).
Clemens, H.P., Inslee, T., 1968. The production of unisexual broods by Tilapia mossambica sex-reversed with methyltestosterone. Trans. Am. Fish. Soc. 97 (1), 18-21.
Conover, D.O., Heins, S.W., 1987. Adaptative variation in environmental and genetic sex determination in a fish. Nature 326, 496-498.
Desprez, D., Mélard, C., 1998. Effect of ambient temperature on sex determinism in the blue tilapia Oreochromis aureus. Aquaculture 162, 79-84.
Desprez, D., Mélard, C., Philippart, J.C., 1995. Production of a high percentage of male offspring with 17α-ethynylestradiol sex-reversed Oreochromis aureus: II. Comparative reproductive biology of females and F2 pseudofemales and large-scale production of male progeny. Aquaculture 130, 35-41.
Guerrero, R.D., 1975. Use of androgens for the production of all male Tilapia aurea (Steindachner). Trans. Am. Fish. Soc. 104, 342-348.
Guerrero, R.D., Shelton, W.L., 1974. An aceto-carmine squash method for sexing juvenile fishes. Prog. Fish-Cult. 36, 56.
Hulata, G., Wohlfarth, G.W., Karplus, I., Schroder, G.L., Harpaz, S., Halevy, A., Rothbard, S., Cohen, S., Israel, I., Kavessa, M., 1993. Evaluation of Oreochromis niloticus x Oreochromis aureus hybrid progeny of different geographical isolates, reared under varying management regimes. Aquaculture 115, 253-271.
Jalabert, B., Moreau, J., Planquette, P., Billard, R., 1974. Déterminisme du sexe chez Tilapia macrochir et Tilapia nilotica: action de la methyltestostérone dans l'alimentation des alevins sur la différenciation sexuelle; proportion des sexes dans la descendance des mâles "inversés". Ann. Biol. Anim. Biochim. Biophys. 14 (4-B), 729-739 (in French with English abstract).
Jensen, G.L., Shelton, W.L., 1979. Effects of estrogens on Tilapia aurea: implications for production of monosex genetic male tilapia. Aquaculture 16, 233-242.
Kallman, K.D., 1984. A new look at sex determination in poeciliid fishes. In: Turner, B.J. (Ed.), Evolutionary Genetics of Fishes. Plenum, New York.
Lahav, E., 1993. Use of sex-reversed females to produce all-male tilapia (Oreochromis aureus) fry. Isr. J. Aquac.-Bamidgeh 45, 131-136.
Lester, L.J., Lawson, K.S., Abella, T.A., Palada, M.S., 1989. Estimated heritability of sex-ratio and sexual dimorphism in tilapia. Aquac. Fish. Manage. 20, 369-380.
Mair, G.C., Penman, D.J., Scott, A., Skibinski, D.O.F., Beardmore, J.A., 1987. Hormonal sex-reversal and the mechanism of sex determination in Oreochromis. In: Tiews, K. (Ed.), Proceedings of Selection, Hybridisation and Genetic Engineering in Aquaculture, 27-30 May 1986, Bordeaux, France, Vol. 2, Berlin 1987, pp. 301-312.
Mair, G.C., Scott, A.G., Penman, D.J., Skibinski, D.O.F., Beardmore, J.A., 1991a. Sex determination in the genus Oreochromis: 1. Sex reversal, gynogenesis and triploidy in O. niloticus (L). Theor. Appl. Genet. 82, 144-152.
Mair, G.C., Scott, A.G., Penman, D.J., Skibinski, D.O.F., Beardmore, J.A., 1991b. Sex determination in the genus Oreochromis: 2. Sex reversal, hybridisation, gynogenesis and triploidy in O. aureus Steindachner. Theor. Appl. Genet. 82, 153-160.
Mair, G.C., Abucay, J.S., Skibinski, D.O.F., Beardmore, J.A., 1997. Genetic manipulation of sex ratio for the large scale production of all-male tilapia, Oreochromis niloticus. Can. J. Fish. Aquat. Sci. 54, 396-404.
Majumdar, K.C., Mc Andrew, B.J., 1983. Sex ratio from interspecific crosses within the tilapias. In: Fishelson, L., Yaron, Z. (Eds.), Proceedings of the First International Symposium on Tilapia in Aquaculture. Tel Aviv University, Tel Aviv, pp. 261-269.
Mélard, C., 1995. Production of a high percentage of male offspring with 17α-ethynylestradiol sex reversed Oreochromis aureus: I. Estrogen sex reversal and production of F2 pseudofemales. Aquaculture 130, 25-34.
Müller-Belecke, A., Hörstgen-Schwark, G., 1995. Sex determination in tilapia (Oreochromis niloticus) sex ratios in homozygous gynogenetic progeny and their offspring. Aquaculture 137, 57-65.
Pham, A.T., Mair, G.C., Little, D.C., Beardmore, J.A., 1999. Sex determination and the feasibility of genetically male tilapia production in the Thai-Chitralada strain of Oreochromis niloticus. Aquaculture 173, 257-269.
Price, D.J., 1984. Genetics of sex-determination in fishes - a brief review. In: Potts, G.W., Wooton, R.J. (Eds.), Fish Reproduction: Strategies and Tactics. Academic Press, New York, pp. 77-89.
Römer, U., Beisenherz, W., 1996. Environmental determination of sex in Apogramma (Cichlidae) and two other freshwater (Teleostei). J. Fish Biol. 48, 714-725.
Rubin, D.A., 1985. Effect of pH on sex ratio in Cichlids and a poeciliid (Teleostei). Copeia 1, 233-235.
Scott, A.G., Penman, D.J., Beardmore, J.A., Skibinski, D.O.F., 1989. The YY supermale in Oreochromis niloticus (L.) and its potential in aquaculture. Aquaculture 78, 237-251.
Shelton, W.L., Meriwether, F.H., Kenneth, J.S., Calhoun, E.W., 1983. Progeny sex-ratio from intraspecific pair spawning of Tilapia aurea and Tilapia nilotica. In: Fishelson, L., Yaron, Z. (Eds.), Proceeding of the First International Symposium on Tilapia in Aquaculture. Tel Aviv University, Tel Aviv, pp. 270-280.
Wohlfarth, G.W., Wedekind, H., 1991. The heredity of sex determination in tilapias. Aquaculture 92, 143-156.
Yamamoto, T., 1969. Sex differentiation. In: Hoar, W.S., Randall, D.J. (Eds.), Fish Physiology, vol. III. Academic Press, New York, pp. 117-175.