Article (Scientific journals)
Bovine Herpesvirus Type 1 Glycoprotein H Is Essential for Penetration and Propagation in Cell Culture
Meyer, G.; Hanon, E.; Georlette, D. et al.
1998In Journal of General Virology, 79 ((Pt 8)), p. 1983-7
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Abstract :
[en] Bovine herpesvirus type 1 (BHV-1) glycoprotein H (gH) is a structural component of the virion which forms a complex with glycoprotein gL. To study the role of BHV-1 gH in the virus infectious cycle, a gH null mutant was constructed in which the gH coding sequences were deleted and replaced by the Escherichia coli lacZ cassette. The BHV-1 gH null mutant was propagated in trans-complementing MDBK cells, stably transfected with plasmid pMEP4 containing the BHV-1 gH gene under the control of the inducible mouse metallothionein promoter. Experiments with the BHV-1 gH null mutant showed that gH is essential in the infectious cycle of the virus and is specifically involved in virus entry and cell-to-cell spread. The lack of infectivity of virions devoid of gH is not due to a defect in attachment. Moreover, PEG-induced fusion of virions to target cells provides evidence that BHV-1 gH is required for virion penetration.
Disciplines :
Microbiology
Veterinary medicine & animal health
Author, co-author :
Meyer, G.
Hanon, E.
Georlette, D.
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Language :
English
Title :
Bovine Herpesvirus Type 1 Glycoprotein H Is Essential for Penetration and Propagation in Cell Culture
Publication date :
August 1998
Journal title :
Journal of General Virology
ISSN :
0022-1317
eISSN :
1465-2099
Publisher :
Society for General Microbiology, United Kingdom
Volume :
79
Issue :
(Pt 8)
Pages :
1983-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 March 2009

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