Article (Scientific journals)
Gene expression profile of human lymphocytes exposed to (211)At alpha particles
Turtoi, Andrei; Brown, Ian; Schläger, Martin et al.
2010In Radiation Research, 174, p. 125-136
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Abstract :
[en] In this study, the Whole Human Genome 44K DNA microarray assay was used for the first time to obtain gene expression profiles in human peripheral blood lymphocytes 2 h after exposure (in suspension) to 6.78 MeV mean energy alpha particles from extracellular (211)At. Lymphocytes were exposed to fluences of 0.3-9.6 x 10(6) alpha particles/cm(2) [corresponding to mean absorbed alpha-particle doses (D(alpha)) of 0.05-1.60 Gy] over 30 min. Significantly modulated expression was identified in 338 early-response genes. Up-regulated expression was evident in 183 early-response genes, while the remaining 155 were down-regulated. Over half of the up-regulated genes and 40% of the down-regulated genes had a known biological process related primarily to cell growth and maintenance and cell communication. Genes associated with cell death were found only in the up-regulated genes and those with development only in the down-regulated genes. Eight selected early-response genes that displayed a sustained up- or down-regulation (CD36, HSPA2, MS4A6A, NFIL3, IL1F9, IRX5, RASL11B and SULT1B1) were further validated in alpha-particle-irradiated lymphocytes of two human individuals using the TaqMan(R) RT-qPCR technique. The results confirmed the observed microarray gene expression patterns. The expression modulation profiles of IL1F9, IRX5, RASL11B and SULT1B1 genes demonstrated similar trends in the two individuals studied. However, no significant linear correlation between increasing relative gene expression and the alpha-particle dose was evident. The results suggest the possibility that a panel of genes that react to alpha-particle radiation does exist and that they merit further study in a greater number of individuals to determine their possible value regarding alpha-particle biodosimetry.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Turtoi, Andrei ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Brown, Ian;  UHCW Warwick and University of Cambridge, United Kingdom
Schläger, Martin;  Forschungszentrum Juelich, Department of Safety and Radiation Protection, Germany
Schneeweiss, Frank HA;  Forschungszentrum Juelich, Department of Safety and Radiation Protection, Germany
Language :
English
Title :
Gene expression profile of human lymphocytes exposed to (211)At alpha particles
Publication date :
August 2010
Journal title :
Radiation Research
ISSN :
0033-7587
Publisher :
Kluge Carden Jennnings Pub Co, Charlottesville, United States - Virginia
Volume :
174
Pages :
125-136
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 November 2010

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