Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.

Franceschi, V.; Capocefalo, A.; Ravanetti, L.; Vanderplasschen, Alain; Gillet, Laurent; Cavirani, S.; van Santen, V. L.; Donofrio, G.

doi:10.1016/j.vetmic.2010.09.031

Article (Scientific journals)

Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.

Franceschi, V.; Capocefalo, A.; Ravanetti, L. et al.

2011 • In Veterinary Microbiology

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/76183

DOI
10.1016/j.vetmic.2010.09.031

PubMed
21035279

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Abstract :

[en] The ORF50/Rta gene has been shown to be an essential gene for many gammaherpesviruses. Although the BoHV-4 ORF50/Rta homolog, immediate early gene 2 (IE2), has been shown to activate several BoHV-4 early and late promoters in cotransfection assays, there is no direct proof of its indispensability for progression of the virus to the lytic replication cycle in the context of the viral genome. In the present communication, replication defective BoHV-4-V.test IE2 mutants were efficiently rescued, with respect to production of infectious virus and DNA replication, upon the expression of BoHV-4 ORF50/Rta in trans. Surprisingly, in the course of our studies, we discovered that the IE2 gene is duplicated in the genome of BoHV-4-U.

Disciplines :

Microbiology

Author, co-author :

Franceschi, V.

Capocefalo, A.

Ravanetti, L.

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie

Cavirani, S.

van Santen, V. L.

Donofrio, G.

Language :

English

Title :

Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.

Publication date :

2011

Journal title :

Veterinary Microbiology

ISSN :

0378-1135

eISSN :

1873-2542

Publisher :

Elsevier Science, Amsterdam, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 20 November 2010

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