Reference : Generation of a transposon insertion mutant library for bovine herpesvirus 4 cloned a...
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Microbiology
Generation of a transposon insertion mutant library for bovine herpesvirus 4 cloned as a bacterial artificial chromosome by in vitro MuA based DNA transposition system.
Donofrio, Gaetano [> > > >]
Martignani, Eugenio [> > > >]
Sartori, Chiara [> > > >]
Vanderplasschen, Alain mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Cavirani, Sandro [> > > >]
Flammini, Cesidio Filippo [> > > >]
Gillet, Laurent mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Journal of Virological Methods
Elsevier Science
Yes (verified by ORBi)
The Netherlands
[en] Animals ; Catalysis ; Cattle ; Cell Line ; Chromosomes, Artificial, Bacterial ; Cloning, Molecular ; DNA Transposable Elements ; DNA, Viral ; Electroporation ; Escherichia coli/genetics ; Gene Library ; Herpesvirus 4, Bovine/genetics ; Models, Genetic ; Mutagenesis, Insertional ; Transposases/metabolism
[en] Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus with no clear disease association. Although the BoHV-4 genome has been sequenced, the function of the majority of putative genes is elusive. Several features make BoHV-4 attractive as a backbone for use as a viral expression vector and/or as a model to study gamma herpesvirus biology and determining which genes are essential for its replication is a very important task. Starting from BoHV-4 genome cloned as infectious bacterial artificial chromosome (BAC-BoHV-4) in Escherichia coli. A random insertion mutant library for BoHV4 was generated by the use of MuA transposase-catalyzed in vitro transposition reaction. Viral mutant transfection and direct sequencing allow the rapid determination of which BoHV-4 genes are essential for viral growth in a permissive eukaryotic cell line. BoHV-4 functional analysis information is fundamental when the BoHV-4 genome is modified for vector purposes.
Researchers ; Professionals

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