Reference : Recombinant bovine herpesvirus 4 (BoHV-4) expressing glycoprotein D of BoHV-1 is immu...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Microbiology
http://hdl.handle.net/2268/76179
Recombinant bovine herpesvirus 4 (BoHV-4) expressing glycoprotein D of BoHV-1 is immunogenic and elicits serum-neutralizing antibodies against BoHV-1 in a rabbit model.
English
Donofrio, Gaetano [> > > >]
Cavirani, Sandro [> > > >]
Vanderplasschen, Alain mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Gillet, Laurent mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Flammini, Cesidio Filippo [> > > >]
2006
Clinical and Vaccine Immunology
American Society for Microbiology (ASM)
13
11
1246-54
Yes (verified by ORBi)
International
1556-6811
1556-679X
Washington
DC
[en] Animals ; Antibodies, Viral/biosynthesis/blood ; Cattle ; Cell Line ; Genetic Vectors ; Herpesvirus 1, Bovine/immunology ; Herpesvirus 4, Bovine/genetics/immunology ; Neutralization Tests ; Plasmids ; Rabbits ; Viral Proteins/genetics/immunology
[en] Several biological characteristics of bovine herpesvirus 4 (BoHV-4) make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types coming from different animal species, and the ability to maintain transgene expression in both undifferentiated and differentiated cells. Starting from BoHV-4 cloned as a bacterial artificial chromosome (BAC), we used MuA transposase-mediated in vitro transposition to generate recombinant BoHV-4 expressing the immunodominant glycoprotein D (gD) of BoHV-1, one of the most important pathogens of cattle. Although a cis-acting element from woodchuck hepatitis virus (the woodchuck hepatitis virus posttranscriptional regulatory element [WPRE]) in the 3' end of the gD expression cassette was required for maximal gD expression from plasmids in transient transfection assays, this element was not necessary for efficient expression of gD from recombinant BoHV-4 genomes. BoHV-4 recombinants containing gD expression cassettes with or without the WPRE expressed gD at similarly high levels. Several cell lines originating from different animal species expressed gD when infected with BoHV-4 recombinants. When rabbits were immunized with one of the recombinants, high levels of serum neutralizing antibodies against BoHV-1 were generated. This work is one of the first demonstrations of the use BoHV-4 as a vector for vaccine purposes and may provide the basis for BoHV-1 vaccination of cattle with recombinant BoHV-4.
Researchers ; Professionals
http://hdl.handle.net/2268/76179
also: http://hdl.handle.net/2268/29128
10.1128/CVI.00200-06

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
CVI 2006 1246.pdfPublisher postprint755.35 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.