Reference : Murine gammaherpesvirus-68 glycoprotein B presents a difficult neutralization target ...
Scientific journals : Article
Life sciences : Microbiology
Murine gammaherpesvirus-68 glycoprotein B presents a difficult neutralization target to monoclonal antibodies derived from infected mice.
Gillet, Laurent mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Gill, Michael B [> > > >]
Colaco, Susanna [> > > >]
Smith, Christopher M [> > > >]
Stevenson, Philip G [> > > >]
Journal of General Virology
Society for General Microbiology
Pt 12
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Antibodies, Monoclonal/immunology/isolation & purification ; Antibodies, Viral/immunology/isolation & purification ; Antigens, Viral/immunology ; Cell Line ; Cricetinae ; Disease Models, Animal ; Female ; Herpesviridae Infections/immunology ; Immunoglobulin M/immunology/isolation & purification ; Lung/virology ; Membrane Fusion ; Mice ; Mice, Inbred BALB C ; Neutralization Tests ; Plaque Assay ; Rhadinovirus/immunology/physiology ; Viral Envelope Proteins/immunology ; Virus Internalization
[en] Persistent viruses disseminate from immune hosts. They must therefore resist neutralization by antibody. Murine gammaherpesvirus-68 (MHV-68) represents an accessible model with which to address how resistance to neutralization is achieved and how overcoming it might improve infection control. The MHV-68 glycoprotein B (gB), like that of other herpesviruses, is a virion protein that is essential for infectivity. As such, it presents a potential neutralization target. In order to test whether virus-induced antibodies reduce virion infectivity by binding to gB, monoclonal antibodies (mAbs) were derived from MHV-68-infected mice. gB-specific mAbs were common, but only an IgM specific for the gB N terminus reduced virion infectivity significantly. It inhibited MHV-68 entry into BHK-21 cells at a post-binding step that was linked closely to membrane fusion. Reducing the mAb to IgM monomers compromised neutralization severely, suggesting that a pentameric structure was crucial to its function. Antibody treatment never blocked BHK-21 cell infection completely and blocked the infection of NMuMG epithelial cells hardly at all. Virions saturated with antibody also remained infectious to mice. Thus, the MHV-68 gB presents at best a very difficult target for antibody-mediated neutralization.

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