Article (Scientific journals)
Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor
De Veylder, L.; Beeckman, T.; Beemster, G. T. S. et al.
2002In EMBO Journal, 21 (6), p. 1360-1368
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Keywords :
Arabidopsis thaliana; cell cycle; cell differentiation; E2F-DP transcription; endoreduplication
Abstract :
[en] New plant cells arise at the meristems, where they divide a few times before they leave the cell-cycle program and start to differentiate. Here we show that the E2Fa-DPa transcription factor of Arabidopsis thaliana is a key regulator determining the proliferative status of plant cells. Ectopic expression of E2Fa induced sustained cell proliferation in normally differentiated cotyledon and hypocotyl cells. The phenotype was enhanced strongly by the co-expression of E2Fa with its dimerization partner, DPa. In endoreduplicating cells, E2Fa-DPa also caused extra DNA replication that was correlated with transcriptional induction of S phase genes. Because E2Fa-DPa transgenic plants arrested early in development, we argue that controlled exit of the cell cycle is a prerequisite for normal plant development.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
De Veylder, L.
Beeckman, T.
Beemster, G. T. S.
Engler, J. D.
Ormenese, Sandra ;  Université de Liège - ULiège > GIGA-Management : Plate-forme imagerie
Maes, S.
Naudts, M.
Van der Schueren, E.
Jacqmard, Annie ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Engler, G.
Inze, D.
Language :
English
Title :
Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor
Publication date :
15 March 2002
Journal title :
EMBO Journal
ISSN :
0261-4189
eISSN :
1460-2075
Publisher :
Oxford Univ Press, Oxford, United Kingdom
Volume :
21
Issue :
6
Pages :
1360-1368
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 November 2010

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