Animals; Biological Markers/metabolism; Bone and Bones/metabolism/radiography; Horse Diseases/metabolism/radiography; Horses/physiology; Joint Diseases/metabolism/radiography/veterinary; Joints/metabolism; Male; Osteochondrosis/metabolism/radiography/veterinary; Radiography/methods/veterinary
Abstract :
[en] Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could reveal to be an interesting alternative to this procedure which is time consuming and sometimes technically difficult. The aim of this study was to investigate the relationship between the radiological status of the horses and the levels of biochemical markers of cartilage degradation and synovial inflammation. A specific radiological scoring and classification system was therefore developed and applied on 63 stallions presented for studbook admission. Additionally, groups of horses were established according to the occurrence of osteochondrosis, degenerative joint disease and distal interphalangeal joint effusion. Insulin growth factor-I, myeloperoxidases, Coll2-1 and Coll2-1NO(2) were used as blood markers. The combination of the blood parameters did not seem to correlate with the used scoring system. Coll2-1NO(2) levels however tended to increase with poorer radiological class and this could therefore potentially be a useful predictor of the osteoarticular status in the horse. Coll2-1 levels were significantly higher in the degenerative joint disease group. A high percentage of horses with distal interphalangeal joint effusion was present in this study and was associated with decreased IGF-I and increased Coll2-1 levels.
Disciplines :
Rheumatology
Author, co-author :
Verwilghen, Denis ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Busoni, Valeria ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Imagerie médicale
Gangl, Monika
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Lejeune, Jean-Philippe ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Vander Heyden, Laurent ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Detilleux, Johann ; Université de Liège - ULiège > Département de productions animales > Génétique quantitative - Thériogenologie des animaux de production
Grulke, Sigrid ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Deberg, Michelle ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Henrotin, Yves ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) - Didactique des sciences de la santé - Pathologie générale et physiopathologie
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Relationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions
Publication date :
2009
Journal title :
Research in Veterinary Science
ISSN :
0034-5288
eISSN :
1532-2661
Publisher :
British Veterinary Association, London, United Kingdom
Art T., Franck T., Gangl M., Votion D., Kohnen S., Deby-Dupont G., and Serteyn D. Plasma concentrations of myeloperoxidase in endurance and 3-day event horses after a competition. Equine Veterinary Journal Supplement (2006) 298-302
Art T., Franck T., Lekeux P., de Moffarts B., Couetil L., Becker M., Kohnen S., Deby-Dupont G., and Serteyn D. Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction. Canadian Journal of Veterinary Research 70 (2006) 291-296
Baldus S., Eiserich J.P., Brennan M.L., Jackson R.M., Alexander C.B., and Freeman B.A. Spatial mapping of pulmonary and vascular nitrotyrosine reveals the pivotal role of myeloperoxidase as a catalyst for tyrosine nitration in inflammatory diseases. Free Radical Biology and Medicine 33 (2002) 1010-1019
Beckman J.S., and Koppenol W.H. Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and the ugly. American Journal of Physiology-Cell Physiology 40 (1996) C1424-C1437
Billinghurst R.C., Brama P.A., van Weeren P.R., Knowlton M.S., and McIlwraith C.W. Evaluation of serum concentrations of biomarkers of skeletal metabolism and results of radiography as indicators of severity of osteochondrosis in foals. American Journal of Veterinary Research 65 (2004) 143-150
Billinghurst R.C., Dahlberg L., Ionescu M., Reiner A., Bourne R., Rorabeck C., Mitchell P., Hambor J., Diekmann O., Tschesche H., Chen J., Van Wart H., and Poole A.R. Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage. Journal of Clinical Investigation 99 (1997) 1534-1545
Brennan M.L., Wu W.J., Fu X.M., Shen Z.Z., Song W., Frost H., Vadseth C., Narine L., Lenkiewicz E., Borchers M.T., Lusis A.J., Lee J.J., Lee N.A., Abu-Soud H.M., Ischiropoulos H., and Hazen S.L. A tale of two controversies - defining both the role of peroxidases in nitrotyrosine formation in vivo using eosinophil peroxidase and myeloperoxidase-deficient mice, and the nature of peroxidase-generated reactive nitrogen species. Journal of Biological Chemistry 277 (2002) 17415-17427
Burner U., Furtmuller P.G., Kettle A.J., Koppenol W.H., and Obinger C. Mechanism of reaction of myeloperoxidase with nitrite. Journal of Biological Chemistry 275 (2000) 20597-20601
Courouce-Malblanc A., Leleu C., Bouchilloux M., and Geffroy O. Abnormal radiographic findings in 865 French standardbred trotters and their relationship to racing performance. Equine Veterinary Journal Supplement (2006) 417-422
Davicco M.J., Faulconnier Y., Coxam V., Dubroeucq H., Martin-Rosset W., and Barlet J.P. Systemic bone growth factors in light breed mares and their foals. Archives Internationales de Physiologie, de Biochimie et de Biophysique 102 (1994) 115-119
Deberg M., Dubuc J.E., Labasse A., Sanchez C., Quettier E., Bosseloir A., Crielaard J.M., and Henrotin Y. One year follow-up of Coll2-1, Coll2-1NO2 and myeloperoxydase serum levels in oa patients after hip or knee replacement. Annals of the Rheumatic Diseases 67 (2008) 168-174
Deberg M., Labasse A., Christgau S., Cloos P., Henriksen D.B., Chapelle J.P., Zegels B., Reginster J.Y., and Henrotin Y. New serum biochemical markers (Coll 2-1 and Coll 2-1 NO2) for studying oxidative-related type II collagen network degradation in patients with osteoarthritis and rheumatoid arthritis. Osteoarthritis and Cartilage 13 (2005) 258-265
Dik K.J., Enzerink E., and van Weeren P.R. Radiographic development of osteochondral abnormalities, in the hock and stifle of Dutch Warmblood foals, from age 1 to 11 months. Equine Veterinary Journal Supplement 31 (1999) 9-15
Donabedian M., van Weeren P.R., Perona G., Fleurance G., Robert C., Leger S., Bergero D., Lepage O., and Martin-Rosset W. Early changes in biomarkers of skeletal metabolism and their association to the occurrence of osteochondrosis (OC) in the horse. Equine Veterinary Journal 40 (2008) 253-259
Fortier L.A., Balkman C.E., Sandell L.J., Ratcliffe A., and Nixon A.J. Insulin-like growth factor-I gene expression patterns during spontaneous repair of acute articular cartilage injury. Journal of Orthopaedic Research 19 (2001) 720-728
Franck T., Grulke S., Deby-Dupont G., Deby C., Duvivier H., Peters F., and Serteyn D. Development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood. Journal of Veterinary Diagnostic Investigation 17 (2005) 412-419
Gangl, M., Franck, T., Lejeune, J., Vanderheyden, L., Grulke, S., Verwilghen, D., Deberg, M., Henrotin, Y., Serteyn, D., 2008. Variations plasmatiques de quatre marqueurs du métabolisme cartilagineux et de l'inflammation chez les chevaux atteints d'ostéochondrose. 34ème journée de la recherche équine, Paris, pp. 87-93.
Gangl M., Serteyn D., Lejeun J.P., Schneider N., Grulke S., Peters F., Vila T., Deby-Dupont G., Deberg M., and Henrotin Y. A type II-collagen derived peptide and its nitrated form as new markers of inflammation and cartilage degradation in equine osteochondral lesions. Research in Veterinary Science 82 (2007) 68-75
Gaut J.P., Byun J., Tran H.D., Lauber W.M., Carroll J.A., Hotchkiss R.S., Belaaouaj A., and Heinecke J.W. Myeloperoxidase produces nitrating oxidants in vivo. Journal of Clinical Investigation 109 (2002) 1311-1319
Grondahl A.M. The incidence of osteochondrosis in the tibiotarsal joint of norwegian standard-bred trotters - a radiographic study. Journal of Equine Veterinary Science 11 (1991) 272-274
Grondahl A.M. The incidence of bony fragments and osteochondrosis in the metacarpophalangeal and metatarsophalangeal joints of standard-bred trotters - a radiographic study. Journal of Equine Veterinary Science 12 (1992) 81-85
Grulke S., Benbarek H., Caudron I., Deby-Dupont G., Mathy-Hartert M., Farnir F., Deby C., Lamy M., and Serteyn D. Plasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results. Canadian Journal of Veterinary Research 63 (1999) 142-147
Henrotin Y., Addison S., Kraus V., and Deberg M. Type II collagen markers in osteoarthritis: what do they indicate?. Current Opinion in Rheumatology 19 (2007) 444-450
Henrotin Y., Deberg M., Dubuc J.E., Quettier E., Christgau S., and Reginster J.Y. Type II collagen peptides for measuring cartilage degradation. Biorheology 41 (2004) 543-547
Henrotin Y., Debydupont G., Deby C., Debruyn M., Lamy M., and Franchimont P. Production of active oxygen species by isolated human chondrocytes. British Journal of Rheumatology 32 (1993) 562-567
Henrotin Y.E., Zheng S.X., Deby G.P., Labasse A.H., Crielaard J.M.R., and Reginster J.Y.L. Nitric oxide downregulates interleukin 1 beta (IL-1 beta) stimulated IL-6, IL-8, and prostaglandin E-2 production by human chondrocytes. Journal of Rheumatology 25 (1998) 1595-1601
Jackson B.F., Blumsohn A., Goodship A.E., Wilson A.M., and Price J.S. Circadian variation in biochemical markers of bone cell activity and insulin-like growth factor-I in two-year-old horses. Journal of Animal Science 81 (2003) 2804-2810
Jackson B.F., Goodship A.E., Eastell R., and Price J.S. Evaluation of serum concentrations of biochemical markers of bone metabolism and insulin-like growth factor I associated with treadmill exercise in young horses. American Journal of Veterinary Research 64 (2003) 1549-1556
Jeffcott L.B. Osteochondrosis in the horse - searching for the key to pathogenesis. Equine Veterinary Journal 23 (1991) 331-338
Jeffcott L.B. Osteochondrosis - an international problem for the horse industry. Journal of Equine Veterinary Science 16 (1996) 32-37
Kane A.J., Park R.D., McIlwraith C.W., Rantanen N.W., Morehead J.P., and Bramlage L.R. Radiographic changes in thoroughbred yearlings. Part 1: prevalence at the time of the yearling sales. Equine Veterinary Journal 35 (2003) 354-365
Kaur H., and Halliwell B. Evidence for nitric oxide-mediated oxidative damage in chronic inflammation - nitrotyrosine in serum and synovial-fluid from rheumatoid patients. FEBS Letters 350 (1994) 9-12
Kuwabara K., Jyoyama H., Fleisch J.H., and Hori Y. Inhibition of antigen-induced arthritis in guinea pigs by a selective LTB4 receptor antagonist LY293111Na. Inflammation Research 51 (2002) 541-550
Laverty S., Okouneff S., Ionescu M., Reiner A., Pidoux I., Webber C., Rossier Y., Billinghurst R.C., and Poole A.R. Excessive degradation of type II collagen in articular cartilage in equine osteochondrosis. Journal of Orthopaedic Research 20 (2002) 1282-1289
Lejeune J.P., Franck T., Gangl M., Schneider N., Michaux C., Deby-Dupont G., and Serteyn D. Plasma concentration of insulin-like growth factor I (IGF-I) in growing Ardenner horses suffering from juvenile digital degenerative osteoarthropathy. Veterinary Research Communications 31 (2007) 185-195
Lejeune J.P., Serteyn D., Gangl M., Schneider N., Deby-Dupont G., Deberg M., and Henrotin Y. Plasma concentrations of a type II collagen-derived peptide and its nitrated form in growing ardenner sound horses and in horses suffering from juvenile digital degenerative osteoarthropathy. Veterinary Research Communications 31 (2007) 591-601
O'Kane J.W., Hutchinson E., Atley L.M., and Eyre D.R. Sport-related differences in biomarkers of bone resorption and cartilage degradation in endurance athletes. Osteoarthritis and Cartilage 14 (2006) 71-76
Olivier A., Nurton J.P., and Guthrie A.J. An epizoological study of wastage in thoroughbred racehorses in Gauteng, South Africa. Journal of the South African Veterinary Association 68 (1997) 125-129
Philipsson J., Andréasson E., Sandgren B., Dalin G., and Carlsten J. Osteochondrosis in the tarsocrural joint and osteochondral fragments in the fetlock joints in standardbred trotters II. Heritability. Equine Veterinary Journal Supplement 16 (1993) 38-41
Robert C., Valette J.P., and Denoix J.M. Correlation between routine radiographic findings and early racing career in French trotters. Equine Veterinary Journal Supplement (2006) 473-478
Rossdale P.D., Hopes R., Digby N.J., and offord K. Epidemiological study of wastage among racehorses 1982 and 1983. Veterinary Record 116 (1985) 66-69
Schneider N., Mouithys-Mickalad A.L., Lejeune J.P., Deby-Dupont G.P., Hoebeke M., and Serteyn D.A. Synoviocytes, not chondrocytes, release free radicals after cycles of anoxia/re-oxygenation. Biochemical and Biophysical Research Communications 334 (2005) 669-673
Semevolos S.A., Nixon A.J., and Brower-Toland B.D. Changes in molecular expression of aggrecan and collagen types I, II, and X, insulin-like growth factor-I, and transforming growth factor-beta 1 in articular cartilage obtained from horses with naturally acquired osteochondrosis. American Journal of Veterinary Research 62 (2001) 1088-1094
Semevolos, S.A., Nixon, A.J., Strassheim, L., 2004. Unlocking the key to equine articular osteochondrosis: The role of molecular regulators. AAEP Focus on Joints. Louisville, Kentucky, pp. 98-102.
Serteyn D., Grulke S., Franck T., Mouithys-Mickalad A., and Deby-Dupont G. Neutrophile myeloperoxidase, protective enzyme with strong oxidative activities. Annales de Medecine Veterinaire 147 (2003) 79-93
Skioldebrand E., Heinegard D., Olofsson B., Rucklidge G., Roneus N., and Ekman S. Altered homeostasis of extracellular matrix proteins in joints of standardbred trotters during a long-term training programme. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine 53 (2006) 445-449
Sloet van Oldenruitenborgh-Oosterbaan M.M., Mol J.A., and Barneveld A. Hormones, growth factors and other plasma variables in relation to osteochondrosis. Equine Veterinary Journal Supplement 31 (1999) 45-54
Stock K.F., Hamann H., and Distl O. Prevalence of osseous fragments in distal and proximal interphalangeal, metacarpo- and metatarsophalangeal and tarsocrural joints of Hanoverian Warmblood horses. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine 52 (2005) 388-394
Stock K.F., Meiners F., Hamann H., and Distl O. Analysis of the distribution of radiographic findings among Hanoverian Warmblood horses selected for sale by auction. Part 1: osseous fragments in different limb joints. Tierarztliche Praxis Ausgabe Grosstiere Nutztiere 32 (2004) 157-168
Thorp B.H., Ekman S., Jakowlew S.B., and Goddard C. Porcine osteochondrosis: deficiencies in transforming growth factor-beta and insulin-like growth factor-I. Calcified Tissue International 56 (1995) 376-381
Todhunter R.J., and Lust G. Pathophysiology of synovitis - clinical signs and examination in horses. Compendium on Continuing Education for the Practicing Veterinarian 12 (1990) 980-992
van Hoogmoed L.M., Snyder J.R., Thomas H.L., and Harmon F.A. Retrospective evaluation of equine prepurchase examinations performed 1991-2000. Equine Veterinary Journal 35 (2003) 375-381
Wittwer C., Hamann H., Rosenberger E., and Distl O. Prevalence of osteochondrosis in the limb joints of south German coldblood horses. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine 53 (2006) 531-539
Zofkova I. Pathophysiological and clinical importance of insulin-like growth factor-I with respect to bone metabolism. Physiological Research 52 (2003) 657-679
