No full text
Article (Scientific journals)
Relationship between interphasic nucleolar organizer regions and growth rate in two neuroblastoma cell lines.
Derenzini, M.; Pession, A.; Farabegoli, F. et al.
1989In American Journal of Pathology, 134 (4), p. 925-32
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Cell Line; Cell Transformation, Neoplastic/pathology; Chromosomes/analysis/ultrastructure; DNA/analysis/biosynthesis; DNA, Ribosomal/analysis; Humans; Interphase; Karyotyping; Microscopy, Electron; Neuroblastoma/pathology/ultrastructure; Nucleic Acid Hybridization; Nucleolus Organizer Region/ultrastructure
Abstract :
[en] The relationship between the quantity of silver-stained interphasic nucleolar organizer regions (NORs) and nuclear synthetic activity, caryotype, and growth rate was studied in two established neuroblastoma cell lines (CHP 212 and HTB 10). Statistical analysis of silver-stained NORs revealed four times as many in CHP 212 cells compared with HTB 10 cells. No difference was observed in the ribosomal RNA synthesis between the two cell lines. The caryotype index was 1.2 for CHP 212 and 1.0 for HTB 10 cells. The number of chromosomes carrying NORs and the quantity of ribosomal genes was found to be the same for the two cell lines. Doubling time of CHP 212 cells was 20 hours compared with 54 hours for HTB 10 cells. In CHP 212 cells bindering of cell duplication by serum deprivation induced a progressive lowering (calculated at 48, 72, and 96 hours) of the quantity of silver-stained interphasic NORs. Recovery of duplication by new serum addition induced, after 24 hours, an increase of the quantity of silver-stained interphasic NORs up to control levels. In the light of available data, these results indicate that the quantity of interphasic NORs is strictly correlated only to the growth rate of the cell.
Disciplines :
Genetics & genetic processes
Author, co-author :
Derenzini, M.
Pession, A.
Farabegoli, F.
Trere, D.
Badiali, M.
Dehan, Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Language :
English
Title :
Relationship between interphasic nucleolar organizer regions and growth rate in two neuroblastoma cell lines.
Publication date :
1989
Journal title :
American Journal of Pathology
ISSN :
0002-9440
eISSN :
1525-2191
Publisher :
American Society for Investigative Pathology, Bethesda, United States - Maryland
Volume :
134
Issue :
4
Pages :
925-32
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 October 2010

Statistics


Number of views
39 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
123
Scopus citations®
without self-citations
105

Bibliography


Similar publications



Contact ORBi