Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains

Szalo, Ioan Mihai; Goffaux, Frédéric; Pirson, Vinciane; Piérard, Denis; Ball, Hywel; Mainil, Jacques

doi:10.1016/S0923-2508(02)01379-7

Article (Scientific journals)

Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains

Szalo, Ioan Mihai; Goffaux, Frédéric; Pirson, Vinciane et al.

2002 • In Research in Microbiology, 153 (10), p. 653-658

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/7438

DOI
10.1016/S0923-2508(02)01379-7

PubMed
12558184

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Keywords :

Bovine Escherichia coli; EPEC; EHEC; Adhesin

Abstract :

[en] Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC) infections are characterised by the formation of attaching and effacing lesions on intestinal epithelial cells. The first step of EPEC and EHEC pathogenesis involves the initial adherence of the bacterium to the intestinal epithelium. A collection of bovine EPEC and EHEC strains belonging to different serogroups was tested by colony blot hybridization with gene probes for putative adhesins (BFPA, LPFA, IHA, LIFA) of human EPEC and EHEC, and also for fimbrial and afimbrial adhesins (AFA8, F17, Cs31A) of bovine necrotoxigenic E. coli (NTEC). In the bovine EPEC and EHEC strains tested, sequences homologous to lifA, ihA, and lpfA genes were detected, sometimes in association with particular serogroups. Bovine O26 EPEC also possessed a sequence homologous to a gene of the clp operon, coding for the CS31A adhesin, associated with bovine NTEC. Overall results showed that different genes encoding for putative adhesins of human EHEC strains are present in bovine EPEC and EHEC strains, but not one of them is present in all strains.

Disciplines :

Genetics & genetic processes
Microbiology

Author, co-author :

Szalo, Ioan Mihai ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Médecine des oiseaux, des lagomorphes et des rongeurs

Goffaux, Frédéric; The Quenn’s University of Belfast > Department of Veterinary Sciences

Pirson, Vinciane; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes

Piérard, Denis

Ball, Hywel

Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes

Language :

English

Title :

Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains

Publication date :

2002

Journal title :

Research in Microbiology

ISSN :

0923-2508

eISSN :

1769-7123

Publisher :

Elsevier, Paris, France

Volume :

153

Issue :

Pages :

653-658

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

SPF Santé - Service Public Fédéral Santé publique. Sécurité de la Chaîne alimentaire et Environnement
DG RDT - Commission Européenne. Direction Générale de la Recherche et de l'Innovation

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