Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains
Szalo, Ioan Mihai; Goffaux, Frédéric; Pirson, Vincianeet al.
2002 • In Research in Microbiology, 153 (10), p. 653-658
[en] Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC) infections are characterised by the formation of attaching and effacing lesions on intestinal epithelial cells. The first step of EPEC and EHEC pathogenesis involves the initial adherence of the bacterium to the intestinal epithelium. A collection of bovine EPEC and EHEC strains belonging to different serogroups was tested by colony blot hybridization with gene probes for putative adhesins (BFPA, LPFA, IHA, LIFA) of human EPEC and EHEC, and also for fimbrial and afimbrial adhesins (AFA8, F17, Cs31A) of bovine necrotoxigenic E. coli (NTEC). In the bovine EPEC and EHEC strains tested, sequences homologous to lifA, ihA, and lpfA genes were detected, sometimes in association with particular serogroups. Bovine O26 EPEC also possessed a sequence homologous to a gene of the clp operon, coding for the CS31A adhesin, associated with bovine NTEC. Overall results showed that different genes encoding for putative adhesins of human EHEC strains are present in bovine EPEC and EHEC strains, but not one of them is present in all strains.
Disciplines :
Genetics & genetic processes Microbiology
Author, co-author :
Szalo, Ioan Mihai ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Médecine des oiseaux, des lagomorphes et des rongeurs
Goffaux, Frédéric; The Quenn’s University of Belfast > Department of Veterinary Sciences
Pirson, Vinciane; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Piérard, Denis
Ball, Hywel
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains
Publication date :
2002
Journal title :
Research in Microbiology
ISSN :
0923-2508
eISSN :
1769-7123
Publisher :
Elsevier, Paris, France
Volume :
153
Issue :
10
Pages :
653-658
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
SPF Santé - Service Public Fédéral Santé publique. Sécurité de la Chaîne alimentaire et Environnement [BE] DG RDT - Commission Européenne. Direction Générale de la Recherche et de l'Innovation [BE]
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