Article (Scientific journals)
Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation
Vlieghe, Kobe; Vuylsteke, Marnik; Florquin, Kobe et al.
2003In Journal of Cell Science, 116 (20), p. 4249-4259
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Keywords :
Arabidopsis thaliana; cell cycle; E2F; microarray; nitrogen assimilation
Abstract :
[en] dPreviously we have shown that overexpression of the heterodimeric E2Fa-DPa transcription factor in Arabidopsis thaliana results in ectopic cell division, increased endoreduplication, and an early arrest in development. To gain a better insight into the phenotypic behavior of E2Fa-DPa transgenic plants and to identify E2Fa-DPa target genes, a transcriptomic microarray analysis was performed. Out of 4,390 unique genes, a total of 188 had a twofold or more up- (84) or down-regulated (104) expression level in E2Fa-DPa transgenic plants compared to wild-type lines. Detailed promoter analysis allowed the identification of novel E2Fa-DPa target genes, mainly involved in DNA replication. Secondarily induced genes encoded proteins involved in cell wall biosynthesis, transcription and signal transduction or had an unknown function. A large number of metabolic genes were modified as well, among which, surprisingly, many genes were involved in nitrate assimilation. Our data suggest that the growth arrest observed upon E2Fa-DPa overexpression results at least partly from a nitrogen drain to the nucleotide synthesis pathway, causing decreased synthesis of other nitrogen compounds, such as amino acids and storage proteins.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Vlieghe, Kobe;  Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Vuylsteke, Marnik;  Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Florquin, Kobe;  Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Rombauts, Stéphane;  Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Maes, Sara;  Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology
Ormenese, Sandra ;  Université de Liège - ULiège > GIGA-Management : Plate-forme imagerie
Van Hummelen, Paul
Van de Peer, Yves
Inze, Dirk
De Veylder, Lieven
Language :
English
Title :
Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation
Publication date :
15 October 2003
Journal title :
Journal of Cell Science
ISSN :
0021-9533
eISSN :
1477-9137
Publisher :
Company Of Biologists Ltd, Cambridge, United Kingdom
Volume :
116
Issue :
20
Pages :
4249-4259
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 October 2010

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