Article (Scientific journals)
Characterization of two genes encoding the mitochondrial alternative oxidase in Chlamydomonas reinhardtii
Dinant, Monique; Baurain, Denis; Coosemans, Nadine et al.
2001In Current Genetics, 39 (2), p. 101-108
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Abstract :
[en] Two cDNA clones (AOX1 and AOX2) and the corresponding genes encoding the alternative oxidases (AOXs) from Chlamydomonas reinhardtii were isolated and sequenced. The cDNAs, AOX1 and AOX2, contained open reading frames (ORFs) encoding putative proteins of 360 amino acids and 347 amino acids, respectively. For each of the ORFs, a potential mitochondrial-targeting sequence was found in the 5'-end regions. In comparison to AOX enzymes from plants and fungi, the predicted amino acid sequences of the ORFs showed their highest degree of identity with proteins from Aspergillus niger (38.1% and 37.2%) and Ajellomyces capsulatus (37% and 34.9%). Several residues supposed either to be Fe ligands or to be involved in the ubiquinol-binding site were fully conserved in both C. reinhardtii putative AOX proteins. In contrast, a cysteine residue conserved in the sequences of all higher plants and probably involved in the regulation of the enzyme activity was missing both from the AOX1 and AOX2 amino acid sequences and from protein sequences from various other microorganisms. The transcriptional expression of the AOX1 and AOX2 genes in wild-type cells and in mutant cells deficient in mitochondrial complex III activity was also investigated.
Disciplines :
Genetics & genetic processes
Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)
Microbiology
Author, co-author :
Dinant, Monique ;  Université de Liège - ULiège > Département de Botanique > Génétique des microorganismes
Baurain, Denis   ;  Université de Liège - ULiège > Département de Botanique > Génétique des microorganismes
Coosemans, Nadine ;  Université de Liège - ULiège > Département de Botanique > Génétique des microorganismes
Joris, Bernard ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Matagne, René-Fernand ;  Université de Liège - ULiège > Département de Botanique > Génétique des microorganismes
 These authors have contributed equally to this work.
Language :
English
Title :
Characterization of two genes encoding the mitochondrial alternative oxidase in Chlamydomonas reinhardtii
Publication date :
April 2001
Journal title :
Current Genetics
ISSN :
0172-8083
eISSN :
1432-0983
Publisher :
Springer International Publishing A.G., Switzerland
Volume :
39
Issue :
2
Pages :
101-108
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 02 October 2010

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