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A simple method for the preparation of covalently closed circular form of plasmid DNA
Le Brun, J. J.; Rentier-Delrue, Françoise; Mercier, L.
1988In BioTechniques
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Keywords :
Plasmid DNA; Covalently closed circular form; purification
Abstract :
[en] We describe a simple, rapid, inexpensive method for isolation of covalently closed circular plasmid DNA. The method involves the electrophoresis of crude DNA preparations in an agarose gel, electrotransfer onto a dialysis membrane and elution of the highly purified circular covalently closed plasmid DNA. Native and recombinant plasmid DNA have been purified by this method and shown to be suitable for restriction enzyme digestion and transformation of bacteria. The yield of this rapid purification procedure makes it a good alternative method to standard centrifugation in cesium chloride ethidium bromide gradients.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Le Brun, J. J.;  Université d'Angers, France > INSERM U 298
Rentier-Delrue, Françoise ;  Université de Liège - ULiège > Laboratoire Central de Génie génétique
Mercier, L.;  Université d'Angers, France > INSERM U 298
Language :
English
Title :
A simple method for the preparation of covalently closed circular form of plasmid DNA
Publication date :
1988
Journal title :
BioTechniques
ISSN :
0736-6205
eISSN :
1940-9818
Publisher :
Eaton Publishing Company, Natick, United States - Massachusetts
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 October 2010

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