2009 • In proceedings of the International Symposium on Biomechanics and Biology of Bone Regeneration “From Functional Assessment To Guided Tissue Formation
[en] The in vitro engineering of tissues may be achieved by mimicking in vivo tissue development. Although multiple skeletal tissue engineering applications already exist, the underlying mechanisms at protein level are often poorly understood. Growth factors and protein pathways precisely navigate mesenchymal stem cells trough the correct cascades. A detailed understanding of these cascades will enable us to develop efficient and robust production methods, which are required for large scale tissue engineering applications. Multiple studies hypothesize that the Wnt/β-catenin pathway acts as a switching mechanism to determine the differential fate of esenchymal cells in osteoblasts or chondrocytes during skeletogenesis. The concentration of β-catenin is a key factor in this mechanism. Wnt upregulates the productionof β-catenin which in turn upregulates Runx2 and downregulates Sox9. High concentrations of Runx2 relative to Sox9 lead to osteoblasts, while the opposite situation leads to chondrocytes. Crosstalks of the Wnt pathway with other pathways such as that of BMP and ERK expand the amount of factors that can influence β-catenin, turning the linear signaling cascade into a complex network with multiple starting conditions. A mathematical model was derived from literature describing the pathways of BMP, Wnt and ERK as well as various crosstalks between these pathways that were suggested in
literature. CellDesigner™ was used to formulate, solve and visualize the Ordinary Differential Equations describing the temporal evolution of the various model constituents. Multiple starting conditions (various concentrations of BMP, Wnt and ERK) were examined to clarify the crosstalk effect. Modeling various crosstalks proposed in literature resulted in a mutual inhibitory effect between Wnt and BMP signaling, an effect independently described in literature. Experiments are ongoing to corroborate the model predictions.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.