Article (Scientific journals)
New insights into peripherin expression in cochlear neurons
Lallemend, François; Vandenbosch, Renaud; Hadjab, S. et al.
2007In Neuroscience, 150 (1), p. 212-222
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Keywords :
cochlea; peripheral nervous system; axotomy; organotypic culture; regeneration
Abstract :
[en] Peripherin is an intermediate filament protein that is expressed in peripheral and enteric neurons. In the cochlear nervous system, peripherin expression has been extensively used as a differentiation marker by preferentially labeling the type II neuronal population at adulthood, but yet without knowing its function. Since the expression of peripherin has been associated in time with the process of axonal extension and during regeneration of nerve fibers in other systems, it was of interest to determine whether peripherin expression in cochlear neurons was a static phenotypic trait or rather prone to modifications following nerve injury. In the present study, we first compared the expression pattern of peripherin and beta III-tubulin from late embryonic stages to the adult in rat cochlea. The staining for both proteins was seen before birth within all cochlear neurons. By birth, and for 2 or 3 days, peripherin expression was gradually restricted to the type II neuronal population and their projections. In contrast, from postnatal day (P) 10 onwards, while the expression of beta III-tubulin was still found in projections of all cochlear neurons, only the type I population had beta III-tubulin immunoreactivity in their cell bodies. We next investigated the expression of peripherin in axotomized cochlear neurons using an organotypic explant model. Peripherin expression was surprisingly re-expressed in a vast majority of neurons after axotomy. In parallel, the expression and localization of beta III-tubulin and peripherin in dissociated cultures of cochlear neurons were studied. Both proteins were distributed along the entire neuronal length but exhibited complementary distribution, especially within the projections. Moreover, peripherin immunoreactivity was still abundant in the growth cone, whereas that of beta III-tubulin was decreasing at this compartment. Our findings are consistent with a model in which peripherin plays an important structural role in cochlear neurons and their projections during both development and regenerative processes and which is compatible with the assumption that frequently developmentally regulated factors are reactivated during neuronal regeneration.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Lallemend, François
Vandenbosch, Renaud  ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Hadjab, S.
Breuskin, Ingrid ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Moonen, Gustave  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
LEFEBVRE, Philippe 
Malgrange, Brigitte  ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Language :
English
Title :
New insights into peripherin expression in cochlear neurons
Publication date :
30 November 2007
Journal title :
Neuroscience
ISSN :
0306-4522
eISSN :
1873-7544
Publisher :
Pergamon-Elsevier Science Ltd, Oxford, United Kingdom
Volume :
150
Issue :
1
Pages :
212-222
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 October 2008

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