[en] A previous study conducted in Belgium revealed that genetic material of Fasciola sp. was present in snail species belonging to the genus Radix. Here, these snails were collected and identified by DNA-based techniques as Radix labiata and Radix balthica. These two species and Galba truncatula (the major intermediate host in Europe) were experimentally infected with Fasciola hepatica. The resulting metacercariae were fed to rats and the infection was monitored using several techniques. Microscopy revealed the presence of larval stages in 78.3, 45, and 6.25% of G. truncatula, R. labiata, and R. balthica snails, respectively. These results were confirmed by a PCR that amplifies a Fasciola sp. specific sequence. Furthermore, this PCR was found to be more sensitive than microscopic examination. R. labiata shed fewer metacercariae than G. truncatula but these were as infective to rats as those shed by G. truncatula. This study demonstrates that R. labiata may act as an incidental intermediate host for F hepatica in Belgium. (C) 2007 Elsevier B.V. All rights reserved.
Disciplines :
Veterinary medicine & animal health Environmental sciences & ecology Biochemistry, biophysics & molecular biology
Author, co-author :
Caron, Yannick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
LASRI, Saadia ; Centre Hospitalier Universitaire de Liège - CHU > Secteur assurance qualité
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Fasciola hepatica: an assessment on the vectorial capacity of Radix labiata and R. balthica commonly found in Belgium.
Publication date :
21 October 2007
Journal title :
Veterinary Parasitology
ISSN :
0304-4017
eISSN :
1873-2550
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
149
Issue :
1-2
Pages :
95-103
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
ELSEVIER is acknowledged - http://www.elsevier.com/wps/find/homepage.cws_home
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