[en] At Days 26 to 58 after AI, 138 Holstein-Friesian dairy cows were repeatedly examined by ultrasonography, using a 7.5 MHz linear-array rectal transducer. The total calving rate was 37.6% (52/138), and late embryonic mortality occurred 8.6% of the cows (12/138). On the days of ultrasound scanning, blood samples were drawn from the jugular vein for measuring the concentration of bovine pregnancy-specific protein B (bPSPB) and bovine pregnancy-associated glycoprotein 1 (bPAG 1). When compared with calving results, there were no significant differences in accurate diagnosis of pregnant cows were found between the 3 methods. However, when recognition of an embryo proper with a beating heart was used as the criterion for positive ultrasonographic diagnosis significantly fewer (P<0.001) pregnant cows were correctly identified than by the other 2 tests. When compared with the noncalving cows, significantly fewer (P<0.001) false positive diagnoses were made by the 2 ultrasonographic tests than by the PSPB and bPAG 1 tests, while significantly fewer (P<0.001) false positive diagnoses were made by the bPSPB test than by the bPAG 1 test. The accuracy of detecting nonpregnant animals by both protein tests was limited by the relatively long half-life of these proteins after calving and by early embryonic mortality.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Szenci, Otto; University of Veterinary Science, Budapest, Hungary > Dept. of Obstetrics and Reproduction
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Humblot, Patrice; UNCEIA, Maisons-Alfort, France > Services Techniques
Sulon, Joseph ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Sasser, G.; University of Idaho Moscow ID83843, USA > Dept. of Animal and Veterinary Science
Taverne, Marcel; University of Utrecht - Utrecht - The Netherlands > Dept. of Herd Health and Reproduction
Varga, J.; University of Animal Science - Budapest - Hungary > Dept of Obstetrics and Reproduction
Baltusen, R.; University of Utrecht - Utrecht - The Netherlands > Dept of Herd Health and Reproduction
Schekk, Gy; University of Veterinary Science - Budapest - Hungary > Dept of Obstetrics and Reproduction
Language :
English
Title :
Comparison of ultrasonography, bovine pregnancy specific B, and bovine pregnancy-associated glycoprotein 1 test for pregnancy detection in dairy cows
Publication date :
July 1998
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier, New York, United States - New York
Volume :
50
Issue :
1
Pages :
77-88
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Grant number OTKAT016901
Funders :
OTKA - Országos Tudományos Kutatási Alapprogramok IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture F.R.S.-FNRS - Fonds de la Recherche Scientifique
1. Badtram GA, Gaines JD, Thomas CB, Bosu WTK. Factors influencing the accuracy of early pregnancy detection in cattle by real-time ultrasound scanning of the uterus. Theriogenology 1991;35:1153-1167.
2. Boyd JS, Omran SN, Ayliffe TR. Evaluation of real-time B-mode ultrasound scanning for detecting early pregnancy in cows. Vet Rec 1990;127:350-352.
3. Britt JH. Early postpartum breeding in dairy cows. A review. J Dairy Sci 1975;58:266-271.
4. Butler JE, Hamilton WC, Sasser RG, Ruder CA, Hass GM. Williams RJ. Detection and partial partial characterization of two bovine pregnancy-specific proteins. Biol Reprod 1982;26:925-933.
5. Curran S, Pierson RA, and Ginther OJ. Ultrasonographic appearance of the bovine conceptus from days 10 through 20. J Am Vet Med Ass 1986;189:1289-1294.
6. Hanzen C, and Laurent Y. Application de l'echographie bidimensionnelle au diagnostic de gestation et l'evaluation de l'incidence de la mortalit, embryonnaire dans l'espece bovine. Ann Med Vet 1991;134:481-487.
7. Humblot P, Camous S, Martal J, Charlery J, Jeanguyot N, Thibier M., Sasser RG. Pregnancy specific protein b, progesterone concentrations and embryonic mortality during early pregnancy in dairy cows. J Reprod Fertil 1988;83:215-223.
8. Humblot P, Camous S, Martal J, Charlery J, Jeanguyot N, Thibier M., Sasser RG. Diagnosis of pregnancy by radioimmunoassay of a pregnancy-specific protein in the plasma of dairy cows. Theriogenology 1988;30:257-267.
9. Humblot P, Jeanguyot N, Ruder CA, Leriche I, Thibier M, Sasser RG. Accuracy of pregnancy diagnosis by PSPB RIA in the plasma of dairy cows 28 days after AI. Proc 11th ICAR, Vol 2, 1988;94.
10. Kastelic JP, Bergfelt DR, Ginther OJ. Ultrasonic detection of the conceptus and characterisation of intrauterine fluid on Days 10 to 22 in heifers. Theriogenology 1991;35:569-581.
11. Kirakofe GH. Wright JM. Schalles RR. Ruder CA. Paris S. Sasser RG. Pregnancy-specific protein B in serum of postpartum beef cows. J Anim Sci 1993;71:2199-2205
12. Lynch RA, Alexander BM, Sasser RG. The cloning and expression of the pregnancy-specific protein B (bPSPB) gene. Biol Reprod 1992;46(Suppl 1):72 abstr.
13. Pieterse MC, Szenci O, Willemse AH, Bajcsy ACs, Dieleman SJ, Taverne MAM. Early pregnancy diagnosis in cattle by means of linear-array real-time ultrasound scanning of the uterus and a quantitative and qualitative milk progesterone test. Theriogenology 1990;33: 697-707.
14. Ruder CA, Sasser RG. Source of bovine pregnancy-specific protein b (bPSPB) during the postpartum period and estimation of half-life of bPSPB. J Anim Sci 1986;63 (Suppl); 335 abstr.
15. Sasser RG, Alexander BM, Ruder CA. Pregnancy detection in postpartum cows by measurement of pregnancy specific protein B (PSPB). J Anim Sci 1991;69 (Suppl): 660 abstr.
16. Sasser RG, Ruder CA, Ivani KA, Butler JE, Hamilton WC. Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation. Biol Reprod 1986;35:936-942.
17. Semambo DKN, Eckersall PD, Sasser RG, Ayliffe TR. Pregnancy-specific protein B and progesterone in monitoring viability of the embryo in early pregnancy in the cow after experimental infection with Actinomyces pyogenes. Theriogenology 1992;37: 741-748.
18. Steel RGD, Torrie JH. Principles and Procedures of Statistics. A Biometrical Approach. Tokyo: McGraw-Hill Kogakusha, Ltd., 1980.
19. Szenci O, Gyulai Gy, Nagy P, Kovács L, Varga J, Taverne MAM. Effect of uterus position relative to the pelvic intet on the accuracy of early bovine pregnancy diagnosis by means of ultrasonography. Vet Quart 1995;17:37-39.
20. Szenci O, Piros A, Kovács L Early bovine pregnancy diagnosis by a battery operated portable ultrasonic scanner the "Ultra-Scan" Proc 16th World Buiatrics Congress, 1990; 219-223.
21. Szenci O, Taverne MAM, Beckers JF, Sulon J, Varga J, Börzsönyi L, Hanzen Ch, Schekk Gy. Evaluation of false ultrasonographic diagnoses in cows measuring plasma levels of bovine pregnancy associated glycoprotein (bPAG). Vet Rec 1998;142:304-306.
22. Vasquez MI, Horta AEM, Marques CC, Sasser RG, Humblot P. 1995 Levels of bPSPB throughout single and twin pregnancies after AI or transfer of IVM/IVF cattle embryos. Anim Reprod Sci 1995;38:279-289.
23. Wallace JM, Aitken RP, Cheyne MA, Humblot P. Pregnancy-specific protein B and progesterone concentrations in relation to nutritional regimen, placental mass and pregnancy outcome in growing adolescent ewes carying singleton fetuses. J Reprod Fertil 1997;109:53-58.
24. Xie S, Low BG, Kramer KK, Nagel RJ, Anthony RV, Zoli AP, Beckers JF. Roberts RM. Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. Proc Natl Acad Sci USA 1991;88:10247-10251.
25. Zoli AP, Guilbauit LA, Delahaut P, Ortiz WB, Deckers JF,. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 1992;46:83-92.
