Reference : Validation of Creatine, Glycogen and L-(+)-Lactate Determination in Biopsy Samples of...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Validation of Creatine, Glycogen and L-(+)-Lactate Determination in Biopsy Samples of Bovine Musculus Diaphragma
Desmecht, Daniel mailto [Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Linden, Annick mailto [Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage >]
Cuvelier-Klimek, M. [> > > >]
Baldwin, Paule [> > > >]
Van Eenaeme, Christian [> > > >]
Dandrifosse, Guy [Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Lekeux, Pierre mailto [Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie >]
Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine
Blackwell Publishing
Yes (verified by ORBi)
[en] Whereas reliable measurements of diaphragmatic force and electrical activity are now available in calves, evidence that substrate contents in the bovine diaphragm can be accurately measured is still lacking. The purposes of the present study were therefore a) to describe and test the reliability of methods and procedures used for sampling, lyophilisation, extraction and dosage of muscular lactate, glycogen and creatine contents and b) to report the variation in their levels found along the bovine diaphragm and among individuals. The repeatability of the results yielded by a) assaying the extracts, b) processing and assaying the extracts and c) taking samples at different sites in the muscle was assessed. Neither assay, nor processing effects were significant (P < or = 0.05). Substrates were found to be homogeneously distributed within the two muscles studied. Substrate contents expressed per unit of muscle weight or total creatine were neither more or less variable than when expressed per unit of dry matter.
Belgian "Institut pour l'encouragement de la Recherche scientifique dans l'Industrie et l'Agriculture", IRSIA, GRANT N°5475A, Brussels, Belgium
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