Article (Scientific journals)
Combined Effect of Web 2086 (Paf Antagonist) and Ketoprofen (Nsaid) on Paf-Induced Ex Vivo Platelet Aggregation in Bovine
Bastos da Silva, Miriam; Herion, Francine; Raskinet, Renée et al.
1997In Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, 44 (2), p. 65-71
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Keywords :
PAF antogonist; Bovine
Abstract :
[en] The effect of the specific PAF-antagonist WEB 2086, a thieno-triazolo-diazepine, and ketoprofen, a NSAID, was investigated on PAF-induced bovine platelet aggregation measured ex vivo in platelet-rich plasma (PRP). WEB 2086 was infused intravenously over 1 min followed immediately by ketoprofen administration over 1 s (both drugs = 3 mg/kg), in a group of six healthy male Friesian calves. Depending on the PAF concentration, a reversible (10(-8)-10(-9) mol/l) and irreversible (10(-5)-10(-7) mol/l) platelet aggregation was observed. The reversible aggregation was completely blocked by pretreatment of the animal with WEB 2086 and ketoprofen. The inhibitory effects observed during the irreversible aggregation were 47.22%, 54.00% and 88.00% at 10(-5), 10(-6) and 10(-7) mol/l PAF, respectively. Moreover, the aggregation obtained in these condition became reversible. Maximal inhibitory effect of WEB 2086 and ketoprofen on PAF-induced platelet aggregation in calves was observed within 30 min after administration of both drugs. This inhibition persisted even after 24 h and was significantly different from control with P < 0.05. The combined effect of both drugs exceeded the sum of the individual effects (synergism). It was concluded that WEB 2086 and ketoprofen very effectively blocked PAF-induced bovine platelet aggregation in platelet-rich plasma. The study also suggested a synergism between both substances.
Disciplines :
Veterinary medicine & animal health
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Bastos da Silva, Miriam;  Université de Liège - ULiège > Faculty of Veterinary Medicine > Laboratory of Functional Investigation
Herion, Francine ;  Centre Hospitalier Universitaire de Liège - CHU > Thrombosis Hemostasis
Raskinet, Renée ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
David, Jean-Louis ;  Université de Liège - ULiège > Thrombosis Hemostasis
Gustin, Pascal ;  Université de Liège - ULiège > Faculty of Veterinary Medicine > Pharmacology and Toxicologie
Lekeux, Pierre ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Language :
English
Title :
Combined Effect of Web 2086 (Paf Antagonist) and Ketoprofen (Nsaid) on Paf-Induced Ex Vivo Platelet Aggregation in Bovine
Publication date :
1997
Journal title :
Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine
ISSN :
0931-184X
eISSN :
1439-0442
Publisher :
Blackwell Publishing, Berlin, Germany
Volume :
44
Issue :
2
Pages :
65-71
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico [BR]
Funding text :
Bastos is recipient of a grant from CNPq and FCAP Belém-Parà Brazil
Available on ORBi :
since 20 February 2009

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