[en] The effect of the specific PAF-antagonist WEB 2086, a thieno-triazolo-diazepine, and ketoprofen, a NSAID, was investigated on PAF-induced bovine platelet aggregation measured ex vivo in platelet-rich plasma (PRP). WEB 2086 was infused intravenously over 1 min followed immediately by ketoprofen administration over 1 s (both drugs = 3 mg/kg), in a group of six healthy male Friesian calves. Depending on the PAF concentration, a reversible (10(-8)-10(-9) mol/l) and irreversible (10(-5)-10(-7) mol/l) platelet aggregation was observed. The reversible aggregation was completely blocked by pretreatment of the animal with WEB 2086 and ketoprofen. The inhibitory effects observed during the irreversible aggregation were 47.22%, 54.00% and 88.00% at 10(-5), 10(-6) and 10(-7) mol/l PAF, respectively. Moreover, the aggregation obtained in these condition became reversible. Maximal inhibitory effect of WEB 2086 and ketoprofen on PAF-induced platelet aggregation in calves was observed within 30 min after administration of both drugs. This inhibition persisted even after 24 h and was significantly different from control with P < 0.05. The combined effect of both drugs exceeded the sum of the individual effects (synergism). It was concluded that WEB 2086 and ketoprofen very effectively blocked PAF-induced bovine platelet aggregation in platelet-rich plasma. The study also suggested a synergism between both substances.
Disciplines :
Veterinary medicine & animal health Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Bastos da Silva, Miriam; Université de Liège - ULiège > Faculty of Veterinary Medicine > Laboratory of Functional Investigation
Herion, Francine ; Centre Hospitalier Universitaire de Liège - CHU > Thrombosis Hemostasis
Raskinet, Renée ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
ADAMUS, W. S., H. HUER, C.J. MEADE, G. FREY, and H.M. BRECHT, 1988: Inhibitory effect of oral WEB 2086, a novel selective PAF-acether antagonist, on ex-vivo platelet aggregation. Eur. J. Clin. Pharmacol. 35, 237-240.
BASTOS DA SILVA, M., F. HERION, R. RASKINET, J.-L. DAVID, P. GUSTIN, T. GOUGNARD, G. PLOMTEUX, D. DESMECHT, and P. LEKEUX, 1995: Effect of intravenous administration of WEB 2086 on PAF-induced ex-vivo platelet aggregation in healthy Friesian calves. In: Proceedings: XXVth Congress of the World Veterinary Association, Yokohama, Japan, p. 76.
BASTOS DA SILVA, M., F. HERION, R. RASKINET, J.-L. DAVID, P. GUSTIN, C. DESSY, and P. LEKEUX, 1996a: Inhibition of PAF-induced platelet aggregation by WEB 2086, an antagonist to the receptor for Platelet-activating factor, in bovine. J. Vet. Med., A., 43, 399-413.
BASTOS DA SILVA, M., F. HERION, J.-L. DAVID, P. GUSTIN, M. L. VAN DE WEERDT, and P. LEKEUX, 1996b: Effect of intravenous administration of ketoprofen (NSAID) on PAF-induced bovine platelet aggregation. XIXth World Buiatrics Congress, Edinburg, Scotland, pp. 68-70.
BUSSOLINO, F., M. G. PORCELLINI, L. VARESSE, and A. BOSIA, 1987: Intravascular release of platelet activating factor in children with sepsis. Thrombosis Res. 48, 619-620.
CAZENAVE, J.-P., and A. BERETZ, 1988: Médicaments inhibiteurs des fonctions plaquettaires, In: Pharmacologie, Michel Schorderet, Ed. Frison-Roche, Paris, pp. 287-298.
DAVID, J.-L., F. MONFORT, F. HERION, and R. RASKINET, 1979: Compared effects os three dose-levels of Ticlopodine on platelet function in normal subjects. Thrombosis Res. 14, 35-49.
DEMOPOULOS, C. A., R. N. PINCKARD, and D. J. HANAHAN, 1979: Platelet activating factor: evidence for 1 - 0-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine as the active component (a new class of lipid chemical mediators). J. Biol. Chem. 254, 9355-9358.
FOSTER, A. P., P. LEES, M. J. ANDREWS, and F. M. CUNNINGHAM, 1992: Effects of WEB 2086, an antagonist to the receptor for platelet-activating factor (PAF), on PAF-induced responses in the horse. Equine Vet. J. 24, 203-207.
JOBIN, F., 1995: L'hémostase normale. In: L'hémostase, Les Presses de l'Université Laval, Ed. Maloine, Laval, pp. 3-68.
MCALLISTER, C.G., S. J. MORGAN, A. T. BORNE, and R. A. POLLET, 1993: Comparison of adverse effects of phenylbutazone, flunixin meglumine, and ketoprofen in horses. J. Am. Vet. Med. Assoc. 202, 71-77.
SHPIGEL, N.Y., R. CHEN, M. WINKLER, A. SARAN, G. ZIV, and F. LONGO, 1994: Anti-inflammatory ketoprofen in the treatment of field cases of bovine mastitis. Res. Vet. Sci. 56, 62-68.
STEWART, A. G., and G. J. DUSTING, 1988: Characterization of receptores for platelet-activating factor on platelets, polymorphonuclear leukocytes and macrophages. Br. J. Pharmacol. 94, 1225-1233.
STEWART, A. G., and W. A. PHILLIPS, 1989: Intracellular platelet-activating factor regulates eicosanoid generation in guinea pig resident peritoneal macrophages. Br. J. Pharmacol. 98, 141-148.
VAN DE WEERDT, M. L., D. DESMECHT, and P. LEKEUX, 1994: WEB 2086 prevents the PAF-induced pulmonary dysfunctions in calves. In: Proceedings: XVIIIth World Buiatrics Congress, Bologna, Italy, pp. 1239-1242.
VARGAFTIG, B. B., and P. BRAQUET, 1987: PAF-acether today - relevance for acute experimental anaphylaxis. Br. Med. Bull. 43, 312-315.
VARGAFTIG, B. B., J. LEFORT, M. CHIGNARD, and J. BENVENISTE, 1980: Platelet-activating factor induces a platelet-dependent bronchoconstriction unrelated to the formation of prostaglandin derivatives. Eur. J. Pharmacol. 65, 185-192.
VARGAFTIG, B B., M. CHIGNARD, and J. BENVENISTE, 1981: Present concepts on the mechanism of platelet aggregation. Biochem. Pharmacol. 30, 263-271.
VIEIRA, S., 1985: Introdução a Bioestatística. Editora Campus Ltda. 3a. ed. Rio de Janeiro, pp. 223-245.
WILSON, D. Y., and J. A. STICK, 1993: Effect of a specific platelet-activating factor antagonist on cardiovascular and peripheral cellular responses to colonic ischemia and reperfusion in anesthetized ponies. Am. J. Vet. Res. 54, 443-448.
ZIG, G., and F. LONGO, 1991: Comparative clinical efficacy of ketoprofen and flunixin in the treatment of induced E. coli endotoxin mastitis in lactating dairy cows. In. J. ESPINASSE (ed.) Mammites des Vaches Laitieres, pp. 207-208. Société Française de Buiatrie, Paris.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
